FastQCFastQC Report
Wed 12 Apr 2017
000000000-ATLLR_l01n01_2185_day_3_ll-e.35100000088962.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-ATLLR_l01n01_2185_day_3_ll-e.35100000088962.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences108712
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTT15491.4248657001986902No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG10770.9906910000735889No Hit
GAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGA9880.9088233129737288No Hit
GAGCAAAAGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCT8240.7579660019133122No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA4800.44153359334756054No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA4520.41577746706895286No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC4290.39462064905438227No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG3730.34310839649716685No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA3650.3357495032747075No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGC3290.30263448377364044No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT3260.29987489881521817No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG3160.290676282287144No Hit
GTACTATGTTCTTTTCCATGATCGCCTGGTCCAATCGCACGCAAAGAGGG2960.27227904923099566No Hit
GAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTT2910.26767974096695857No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG2900.2667598793141511No Hit
GCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGCT2820.2594009860916918No Hit
GAGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG2740.25204209286923246No Hit
GCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGA2720.25020236956361763No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC2700.2483626462580028No Hit
GAGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGA2530.2327249981602767No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA2480.2281256898962396No Hit
GGATAGATGTACCAGAAGTGCTGCAATCTATTCGAGAAATTTTTCCCTAG2370.21800721171535803No Hit
AGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGC2280.20972845684009123No Hit
CTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGG2250.206968871881669No Hit
CAATTAGCACATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTG2190.20144970196482448No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT2190.20144970196482448No Hit
AAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAAT2000.18397233056148354No Hit
AGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGC1930.17753329899183162No Hit
AGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAG1930.17753329899183162No Hit
GCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTTTC1930.17753329899183162No Hit
TTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCT1910.1756935756862168No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC1910.1756935756862168No Hit
AGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAG1830.16833468246375743No Hit
GGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATG1820.16741482081095005No Hit
GGATCTTCAGTCAATGCACCTGCATCCTTTCCAAGAACTGTCCATTATTC1810.1664949591581426No Hit
GCAAAAGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTC1800.1655750975053352No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA1800.1655750975053352No Hit
GGCTATTGGGCAAGGGGACGTAGTGTTGGTAATGAAACGAAAACGGGACT1730.15913606593568327No Hit
GTCTATATGCGTCTCCACAACTTGAGGGGTTTTCGGCTGAGAAAATTGCT1730.15913606593568327No Hit
ATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACACCATG1670.15361689601883877No Hit
ATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCC1670.15361689601883877No Hit
ATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTG1660.15269703436603135No Hit
GAGCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGCCATGG1620.14901758775480167No Hit
GCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCA1610.14809772610199426No Hit
GGATACACCATGGACATTCGAGAAATTTTTCCCTAGCAGTTCATATAGGA1570.14441827949076458No Hit
GAGCGAAAGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCT1560.1434984178379572No Hit
AATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGT1490.13705938626830524No Hit
ATCTAATAAGAGCACTGACATTGGAAAGTGTGCAGGACCTTACTGGCAAA1450.13337993965707556No Hit
TGTCCATGGTGTATCCTGTTCCTGTTCCATGGCTGTATGGAGGATCTCCA1440.13246007800426815No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT1430.13154021635146074No Hit
CCCTTGGCCTCGATATCGAAACAGCCACTCTTGTTGGGAAACAAATCGTG1410.1297004930458459No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAA1390.12786076974023108No Hit
GTATAAGGGAATGTGGTGCTTATGGCATTTTGCGCTGGAATTTTTAGGAA1350.1241813231290014No Hit
GGCCTCCACCATGCTAGAAATTCCAACCGGTCTCCTATATGAACTGCTAG1340.12326146147619399No Hit
GTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCTAGACACCATG1340.12326146147619399No Hit
TCATATAGGAGACCGGTTGGAATTTCTAGCATGGTGGAGGCCATGGTGTC1320.12142173817057914No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG1320.12142173817057914No Hit
CATATAGACTGTTGAATACAGATTTTGCCAGTAAGGTCCTGCACACTTTC1320.12142173817057914No Hit
GCTTCATATAGCCCCCCAAGATCGAAGGTTCCAGGTTCCAGGTTGTCCCT1310.12050187651777172No Hit
GCTTAATGCTGGGCCATATCTCTTGTCTTCTTTGCCCAAAATGAGAAATC1290.1186621532121569No Hit
GTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCT1260.11590256825373464No Hit
AGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTTT1240.1140628449481198No Hit
AGCAAAAGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTT1240.1140628449481198No Hit
GCGTGAACACAAATCCTAAAATTCCCTTAGTCAGAGGTGACAAGATTGGT1230.11314298329531237No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT1230.11314298329531237No Hit
GTACACATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAATGAAGTGGA1220.11222312164250497No Hit
AGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATC1190.10946353668408271No Hit
ATTTAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATC1190.10946353668408271No Hit
GGATACACCATGGACACAGTAAACAGAACACACCAATACTCAGAAAAGGG1180.10854367503127528No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT1180.10854367503127528No Hit
CCCAATTAGCACATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCAT1170.10762381337846788No Hit
CTCCTCGATTGCTTCATATAGCCCCCCAAGATCGAAGGTTCCAGGTTCCA1170.10762381337846788No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATGTCCATGGTGTATCCT1160.10670395172566047No Hit
GTATATTTTCAGAGACTCGAACCGTGTTACCATTCCATTCAAGTCCTCCG1160.10670395172566047No Hit
AGCGAAAGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTT1140.10486422842004561No Hit
GTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGATC1140.10486422842004561No Hit
TAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATACTC1110.10210464346162337No Hit
GGACAAGTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAA1090.10026492015600855No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATATT100.007082232145.01
CCCCGTC151.2270149E-4145.05
CATAGAA100.007082232145.03
GAGCGAA1000.0130.51
AGTAGAA850.0127.941171
GAGCAAA6400.0120.0781251
TAGTTTT203.8566918E-4108.754
GGTAGTT203.8566918E-4108.752
AGTTTTT203.8566918E-4108.755
GTAGTTT203.8566918E-4108.753
GACAGTA1050.0103.571437
CAAGGTA352.7450733E-7103.571439
ACAGTAT1250.087.08
GTAGAAA1250.087.02
TAGAAAC1350.085.925933
CAAAAGC9000.084.5833364
TTTTTGG1300.083.653851
GTGTTTT353.4234836E-582.857149
AGCAGGC2450.082.857148
AGCGAAA2050.081.341461
Produced by FastQC (version 0.11.2)