FastQCFastQC Report
Wed 12 Apr 2017
000000000-ATLLR_l01n01_2182_day_1_ul-b.3510000008879b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-ATLLR_l01n01_2182_day_1_ul-b.3510000008879b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences77379
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG9561.2354773258894531No Hit
GAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGA8831.1411364840589824No Hit
GTTCATTGATGCTTAATGCTGGGCCATATCTCTTGTCTTCTTTGCCCAAA5880.7598960958399565No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA4330.559583349487587No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTT4060.5246901614133034No Hit
GTTCATCTCTTGTCTTCTTTGCCCAAAATGAGAAATCCTCTCAGGACAGC3730.4820429315447344No Hit
GTACTATGTTCTTTTCCATGATCGCCTGGTCCAATCGCACGCAAAGAGGG2810.3631476240323602No Hit
GAGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG2500.3230850747618863No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA2440.315331032967601No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGC2430.3140386926685535No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC2370.3062846508742682No Hit
GAGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGA2320.2998229493790305No Hit
GAGCAAAAGCAGGGGTCAGGATATGCAGCCGACCTGAAGAGCACACAGAA2280.2946535881828403No Hit
GCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGCT2200.28431486579045995No Hit
AGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGC2070.26751444190284185No Hit
GGATCTTCAGTCAATGCACCTGCATCCTTTCCAAGAACTGTAAGTCGTTT1990.2571757195104615No Hit
GCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGA1910.24683699711808113No Hit
AGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAG1650.21323614934284496No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG1630.21065146874474985No Hit
AGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAG1620.2093591284457023No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA1570.2028974269504646No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT1560.20160508665141705No Hit
AGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGC1500.19385104485713178No Hit
ATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCC1470.18997402395998914No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA1440.1860970030628465No Hit
GTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCCCAGGGAGACT1350.1744659403714186No Hit
GGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATG1290.16671189857713334No Hit
CCCTTACACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA1270.16412721797903826No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA1230.15895785678284804No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC1230.15895785678284804No Hit
GAGCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGCCATGG1210.15637317618475297No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG1180.15249615528761035No Hit
CTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGG1170.15120381498856278No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAA1150.1486191343904677No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT1110.1434497731942775No Hit
ATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCC1100.14215743289522997No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT1060.13698807169903982No Hit
ATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTG1040.1344033911009447No Hit
TCCACCTACTAGTGCTGACCAACAAAGTCTCTATCAGAATGCAGATGCAT1000.12923402990475452No Hit
GAGCGAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA990.12794168960570698No Hit
ATTAGCAATGGTGAAATTTCTCCAACTATTGCTCCCTCCTACATTAGCCT940.12147998811046926No Hit
GTTCACAGCAGTAGGTAAAGAGTTCAACCACCTGGAAAAAAGAATAGAGA930.1201876478114217No Hit
CCCCAACTGCATTTTTGACATCCTCATAAGTATGTCCTGGAAGAGAAGGT920.11889530751237416No Hit
GAGCAAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATACTAGT910.11760296721332662No Hit
ACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCCCAGGG910.11760296721332662No Hit
AAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAAT910.11760296721332662No Hit
GAGATATGGCCCAGCATTAAGCATCAATGAACTGAGCAATCTTGCAAAAG910.11760296721332662No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTA900.11631062691427907No Hit
CCCTTGGCCTCGATATCGAAACAGCCACTCTTGTTGGGAAACAAATCGTG900.11631062691427907No Hit
TGGCAATGAGATACCCAATTACAGCAGACAAGAGAATAATGGACATGATT890.11501828661523153No Hit
CTTACACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACACC890.11501828661523153No Hit
GTATATTTTCAGAGACTCGAACCGTGTTACCATTCCATTCAAGTCCTCCG890.11501828661523153No Hit
GCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAAAC880.11372594631618399No Hit
ATTTAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATC870.11243360601713642No Hit
GGGTTTGTCCTTGTTCATTCCTCTCTGGAATCATGTCCATTATTCTCTTG870.11243360601713642No Hit
ACATAATGGACTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTT850.10984892541904133No Hit
ATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACT840.10855658511999379No Hit
CAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCA840.10855658511999379No Hit
GGCAAGTGCACCAGTTGAATAGCTTAGTGACACCTCCTTGGCCCCATGGA840.10855658511999379No Hit
GTTCATTGCCATCATCCACTTCATTCTGAGTGCGGGGTTCTTCTCTTGCC840.10855658511999379No Hit
AAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGCTTTC830.10726424482094625No Hit
GAGATGAACTGAGCAATCTTGCAAAAGGAGAGAAGGCTAATGTGCTAATT820.1059719045218987No Hit
ACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACCAT820.1059719045218987No Hit
GTAGATGGATGGTGAATGCCCCATAGCACGAGGACTTCTTTCCCTTTATC800.10338722392380362No Hit
GAGCAAAAGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCT800.10338722392380362No Hit
AATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGT780.10080254332570852No Hit
GGATCTTCAGTATTGCCATCATCCACTTCATTCTGAGTGCGGGGTTCTTC780.10080254332570852No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAATTC100.007078309145.04
TTATAGG151.2256563E-4145.03
ATTATAG151.2256563E-4145.02
TAATAAT100.007078309145.02
CCCCCGA100.007078309145.03
GTAATAA100.007078309145.01
GAGCGAA850.0136.47061
GAGCAAA4950.0103.98991
GCAGGTA850.093.823539
AGTAGAA554.0927262E-1092.2727361
GTATACG259.353804E-487.01
CATTATA259.353804E-487.01
GAGATGA259.353804E-487.01
GCAGGGT2600.086.442319
GCGAAAG1350.085.925933
CGAAAGC1350.085.925934
AGCGAAA1350.085.925932
AAGCAGG7500.085.066677
AGCAAAA6100.084.385252
GTAGAAA1000.079.751
Produced by FastQC (version 0.11.2)