FastQCFastQC Report
Wed 12 Apr 2017
000000000-ATLLR_l01n01_2181_day_1_ll-e.351000000887a8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-ATLLR_l01n01_2181_day_1_ll-e.351000000887a8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences73918
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGA7060.9551124218728863No Hit
GAGCAAAAGCAGGAGGAGTGGGAGAGAGGGAGGCACGCTTCCTGCTAAAC4590.620958359262967No Hit
TCTTAGAGCTATTGATGTGTTTTTTCATCTAATTTGGAAAATTTTAGCAA2110.28545144619713736No Hit
GCAAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGACA1960.2651586893584783No Hit
CAATAGGAGAACCAAAAATATGAAAGAACTGAATATAACTTCTAGGACTA1620.21916177385751778No Hit
GGATAAGAATATAAAAAATACGACCACAATAGGAGAACCAAAAATATGAA1520.20563326929841177No Hit
CTTAACAACTGAGCCACCCAGGTGCCCCCAAATCCCTGACTTTAACTGCA1510.20428041884250114No Hit
GAGCAAAAGCAGGGGGAGCAGCAGAGGGAGAGGAGGAAGCAGGCTCCCCA1350.18263481154793149No Hit
GAGCGAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGA1310.17722340972428907No Hit
GAGCAAAAGCAGGCACATACCGAAATACTTAATTTCTGTCTGATTTTTTT1250.16910630698882545No Hit
GTATCTACTGTATCTTACTTTTTCCTCAGGCCACATCATGGATTTTCTTT1200.16234205470927243No Hit
ATATGTATCAGAGTCGTTTTTTTATATTTACCCTTAGTGGAATTCATTGA1120.1515192510619876No Hit
GCAAAAGCAGGAGGAGTGGGAGAGAGGGAGGCACGCTTCCTGCTAAACAA1090.14746069969425582No Hit
AGCAAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGAC1070.14475499878243459No Hit
GAGCGAAAGCAGGAGGAGTGGGAGAGAGGGAGGCACGCTTCCTGCTAAAC1070.14475499878243459No Hit
ATGTATCAGAGTCGTTTTTTTATATTTACCCTTAGTGGAATTCATTGAGC990.13393219513514976No Hit
GAATAAGGATAAGAATATAAAAAATACGACCACAATAGGAGAACCAAAAA980.13257934467923915No Hit
GTAGATACAGAGAAATAAAGATAAAAATTACACTGGCCATTCACCAGAAA970.13122649422332855No Hit
GCCTTAACAGAGACCATAAAAGAAAAGCTCAAGGCACTTGAAGACAGAAA950.12852079331150734No Hit
GAATATAACTTCTAGGACTAAAAAAATACAATATCTGAATTGGAAAAGCC910.12310939148786493No Hit
CTTATTATGAATAAGGATAAGAATATAAAAAATACGACCACAATAGGAGA910.12310939148786493No Hit
GAGTGGGAGAGAGGGAGGCACGCTTCCTGCTAAACAAGGACCCTGATTCG850.1149922887524013No Hit
GATACATATCACACTTGAATTGCTGAAAATAATAATAAAGAAAATCCATG840.1136394382964907No Hit
CTTAACCACTCAGACACCCAGGCATCCCAACATTAAACAGATTTTTACCT810.10958088692875889No Hit
GTATCAGAGTCGTTTTTTTATATTTACCCTTAGTGGAATTCATTGAGCTT760.10281663464920589No Hit
AGCGAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGAC740.10011093373738468No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA1250.0104.41
CAAGGTA259.352138E-487.09
CGAAAGC1500.082.1666644
AGCAAAA4450.081.460672
AGCGAAA1550.079.516132
GCGAAAG1600.077.031243
CAAAAGC4650.076.397844
GAGCAAA4750.074.7894741
GCAAAAG4900.072.53
AGCTATC300.001928631272.59
AAAGCAG7300.065.547956
CATTAAG451.18658405E-464.444442
AAAAGCA5700.062.3245625
ACAAGGG606.7436285E-660.4166683
GAAAGCA2250.054.7777755
GTTTGGC400.006028823654.374996145
GTAGAAA805.7992474E-754.3749962
GAACAAG400.006028823654.3749966
GCAGGCA1650.048.3333329
AAGCAGG10300.046.4563147
Produced by FastQC (version 0.11.2)