FastQCFastQC Report
Wed 12 Apr 2017
000000000-ATLLR_l01n01_2020_day_14_ul-b.351000000889dc.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-ATLLR_l01n01_2020_day_14_ul-b.351000000889dc.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences114808
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGAGGAGTGGGAGAGAGGGAGGCACGCTTCCTGCTAAAC4230.36844122360811093No Hit
GAGCAAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGA3900.33969758205003137No Hit
GAGCAAAAGCAGGGACTTTTGGGCAGCATTAAGATGTATGAGGTGTATTC3280.2856943766984879No Hit
TCTTAGAGCTATTGATGTGTTTTTTCATCTAATTTGGAAAATTTTAGCAA2560.2229809769354052No Hit
GAGCAAAAGCAGGATGAGAGACAGATTTCTGTTCCTTACGCCTTTAGAAT2550.22210995749425128No Hit
CAATAGGAGAACCAAAAATATGAAAGAACTGAATATAACTTCTAGGACTA2380.20730262699463453No Hit
GAGCAAAAGCAGGGGGAGCAGCAGAGGGAGAGGAGGAAGCAGGCTCCCCA2340.2038185492300188No Hit
GTCTAGTTAATAAAGCCCTACTATTCCACCGTGCTAACAGTCCCTTTGAG2320.20207651034771096No Hit
CTTAACAACTGAGCCACCCAGGTGCCCCCAAATCCCTGACTTTAACTGCA2270.19772141314194133No Hit
GTATATAAGCATGCAAATCCATGAATAGCTGAACCAGCAGAGGCCAATTC2150.1872691798480942No Hit
GAGCCGAAGGCAGAGGTCTAACCCACTGAGCCATCCAGGTGCCCCCAAAT2120.18465612152463243No Hit
ATGTATGAGTTACTAATTTCCTGGACATTATCAGCCATTAGCTGGTTTCC2100.18291408264232456No Hit
GCATTAAGATGTATGAGGTGTATTCCTTCCCCATCTTGGGAATTCTAGTT1760.15329942164309107No Hit
GGATAAGAATATAAAAAATACGACCACAATAGGAGAACCAAAAATATGAA1750.15242840220193715No Hit
GGTCTAACCCACTGAGCCATCCAGGTGCCCCCAAATGTTCATTTTTTAAT1740.15155738276078323No Hit
GAATATAACTTCTAGGACTAAAAAAATACAATATCTGAATTGGAAAAGCC1660.14458922723155182No Hit
AGTTAATAAAGCCCTACTATTCCACCGTGCTAACAGTCCCTTTGAGAACT1590.13849209114347433No Hit
GTATCTACTGTATCTTACTTTTTCCTCAGGCCACATCATGGATTTTCTTT1570.13675005226116646No Hit
GATTAGCACACAGTCTAGTTAATAAAGCCCTACTATTCCACCGTGCTAAC1540.13413699393770467No Hit
CCCTAATACTCATGCTGTCTTCAACCAAACTTCCAAAATTTTAACAAAGG1520.13239495505539683No Hit
CTATTCCACCGTGCTAACAGTCCCTTTGAGAACTCAGCCATCCATTACAC1410.12281374120270364No Hit
GCTAAACAAGGACCCTGATTCGGGGCTTGATCCCAGGACTTTGGAATCAT1310.11410354679116438No Hit
ATATGTATCAGAGTCGTTTTTTTATATTTACCCTTAGTGGAATTCATTGA1290.11236150790885652No Hit
ATATTAGGGTGACACGTGCCGTTCTGCATTTTTAAAATTAAATTCCTTAG1250.10887743014424081No Hit
GTAGATACAGAGAAATAAAGATAAAAATTACACTGGCCATTCACCAGAAA1240.10800641070308689No Hit
GGTTATGTATTTGGATGTATTTTATAAAGAAGCCCCTAAATTCCGTATGG1230.10713539126193297No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA950.0129.736851
GAGCAAA7700.0100.746761
AGCAAAA8000.096.06252
GCAAAAG8200.093.719513
CAAAAGC9400.081.755324
AAAAGCA9500.080.894745
AGCGAAA1600.077.031242
AAAGCAG11600.076.875016
GCAGGGG4500.074.1111159
ATCGGCA406.6381144E-572.499996
TCGGCAT502.0027236E-458.07
CCATCGG502.0027236E-458.04
GCGAAAG2150.057.325583
CGAAAGC2200.056.0227244
AAGCAGG16550.055.1963777
GCAGGAT1550.051.451619
AGCAGGG11850.050.780598
ACCATCG604.926687E-448.3333323
CATCGGC604.926687E-448.3333325
GGCATTA657.309374E-444.6153839
Produced by FastQC (version 0.11.2)