FastQCFastQC Report
Wed 12 Apr 2017
000000000-ATLLR_l01n01_2020_day_14_ll-e.351000000887b4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-ATLLR_l01n01_2020_day_14_ll-e.351000000887b4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences101704
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGA7400.7276016675843625No Hit
GAGCAAAAGCAGGAGGAGTGGGAGAGAGGGAGGCACGCTTCCTGCTAAAC4220.41492959962243375No Hit
CTTAACCACTCAGACACCCAGGCATCCCAACATTAAACAGATTTTTACCT3030.29792338551089437No Hit
TCTTAGAGCTATTGATGTGTTTTTTCATCTAATTTGGAAAATTTTAGCAA2540.24974435617084872No Hit
CAATAGGAGAACCAAAAATATGAAAGAACTGAATATAACTTCTAGGACTA2300.2261464642491937No Hit
GAGCAAAAGCAGGGGGAGCAGCAGAGGGAGAGGAGGAAGCAGGCTCCCCA2140.21041453630142376No Hit
CTTAACAACTGAGCCACCCAGGTGCCCCCAAATCCCTGACTTTAACTGCA1910.18779988987650437No Hit
GCAAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGACA1650.16223550696137812No Hit
GTATCTACTGTATCTTACTTTTTCCTCAGGCCACATCATGGATTTTCTTT1610.15830252497443562No Hit
TCCCAGGACCCTGCGATCATGAACTGAGTCAAAGGCAGACACTTAACCAC1570.1543695429874931No Hit
GAGCAAAAGCAGGGATGTGCACAGATACTTGTATACCCATGTTCATAGCA1530.1504365610005506No Hit
GAATATAACTTCTAGGACTAAAAAAATACAATATCTGAATTGGAAAAGCC1440.14158735152993No Hit
GGATAAGAATATAAAAAATACGACCACAATAGGAGAACCAAAAATATGAA1380.13568787854951622No Hit
GAGCGAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGA1360.13372138755604498No Hit
GCATTAAGATGTATGAGGTGTATTCCTTCCCCATCTTGGGAATTCTAGTT1360.13372138755604498No Hit
GTATACATACAATGGCATATTACTTGGTCTTCAGATGGATGGAAATCCTG1360.13372138755604498No Hit
GTAGATACAGAGAAATAAAGATAAAAATTACACTGGCCATTCACCAGAAA1240.12192244159521749No Hit
ATATGTATCAGAGTCGTTTTTTTATATTTACCCTTAGTGGAATTCATTGA1210.11897270510501062No Hit
GAGTGGGAGAGAGGGAGGCACGCTTCCTGCTAAACAAGGACCCTGATTCG1140.11208998662786125No Hit
GAGCAAAAGCAGGCAGAGTGGGAGAGGGAGAAGCAGGCTCCCGCTGAGCA1130.11110674113112562No Hit
AGCAAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGAC1090.10717375914418312No Hit
GTTTAGTACTGTTAAGTACATTCATTGTTGTACAACCTCCAAAACTTTTT1060.10422402265397625No Hit
GCCTTAACAGAGACCATAAAAGAAAAGCTCAAGGCACTTGAAGACAGAAA1060.10422402265397625No Hit
GAGCGAAAGCAGGAGGAGTGGGAGAGAGGGAGGCACGCTTCCTGCTAAAC1060.10422402265397625No Hit
GAATAAGGATAAGAATATAAAAAATACGACCACAATAGGAGAACCAAAAA1040.10225753166050498No Hit
GAGCAAAAGCAGGGACTTTTGGGCAGCATTAAGATGTATGAGGTGTATTC1030.10127428616376938No Hit
GCAAAAGCAGGAGGAGTGGGAGAGAGGGAGGCACGCTTCCTGCTAAACAA1030.10127428616376938No Hit
ACTTACATAAGGTACTTAGAGTAATCAGATTCCTAGAAACACAAAGTAGA1020.10029104066703375No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGACGT100.0070815664145.01
CCCCGAC100.0070815664145.02
GGACGTG100.0070815664145.02
GAGCGAA1300.0111.538461
GGTTAGA203.8559697E-4108.751
AGCGAAA1500.096.666672
CGAAAGC1750.087.04
GTTAGAC259.362347E-487.02
AGCAAAA6100.085.573772
GCGAAAG1750.082.857143
GCAAAAG6650.079.586473
GAGCAAA7100.075.563381
CAAAAGC7150.075.0349664
CCGGGAC406.6344706E-572.54
CGGGACC406.6344706E-572.55
CCTCTAC300.001930728772.51
AAAGCAG9700.069.510316
AAAAGCA8250.064.151525
CGCTCCC350.003557300262.1428576
GCAGGTA350.003557300262.1428579
Produced by FastQC (version 0.11.2)