FastQCFastQC Report
Wed 12 Apr 2017
000000000-ATLLR_l01n01_2017_day_14_ll-e.35100000088ace.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-ATLLR_l01n01_2017_day_14_ll-e.35100000088ace.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences133742
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGAGGAGTGGGAGAGAGGGAGGCACGCTTCCTGCTAAAC5580.41722121696998704No Hit
GAGCAAAAGCAGGGGGAGCAGCAGAGGGAGAGGAGGAAGCAGGCTCCCCA5460.40824871768030985No Hit
GAGCAAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGA5290.3955376770199339No Hit
CAATAGGAGAACCAAAAATATGAAAGAACTGAATATAACTTCTAGGACTA3630.27141810351273343No Hit
TCTTAGAGCTATTGATGTGTTTTTTCATCTAATTTGGAAAATTTTAGCAA3030.22655560706434777No Hit
GAGCAAAAGCAGGATGAGAGACAGATTTCTGTTCCTTACGCCTTTAGAAT2820.2108537333074128No Hit
GGTCTAACCCACTGAGCCATCCAGGTGCCCCCAAATGTTCATTTTTTAAT2690.2011335257435959No Hit
GGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGACAAATAGGGAA2490.18617936026080065No Hit
GGATAAGAATATAAAAAATACGACCACAATAGGAGAACCAAAAATATGAA2480.1854316519866609No Hit
CTTAACAACTGAGCCACCCAGGTGCCCCCAAATCCCTGACTTTAACTGCA2420.18094540234182233No Hit
ATATTAGGGTGACACGTGCCGTTCTGCATTTTTAAAATTAAATTCCTTAG2380.1779545692452633No Hit
GAGCAAAAGCAGGGTGTGGGGAGGCATTTTATTAAAAAAAATTAACAAAC2320.1734683196004247No Hit
ATATTATCTTACCCCTCTGCCATAGGCCTTTATTGTTTAGGTAATTAAAA2260.16898206995558612No Hit
GTATCTACTGTATCTTACTTTTTCCTCAGGCCACATCATGGATTTTCTTT2130.15926186239176923No Hit
GATGTATGTAGTATGATTCCACTTATATAAAAACAAACAAAAAAAGGAAA2050.15328019619865113No Hit
GAATATAACTTCTAGGACTAAAAAAATACAATATCTGAATTGGAAAAGCC2000.14954165482795231No Hit
ATGTAGTATGATTCCACTTATATAAAAACAAACAAAAAAAGGAAAAGGTC2000.14954165482795231No Hit
CCCCACTCAGCAGGGAGCCCAATGTGGGACTCGATCCCAGGACCCTGAGA1760.13159665624859807No Hit
GTATTACTTAAGCAAAAGTGTTCTCTTGGATAACCAATGACCAGGCGATT1740.1301012397003185No Hit
GCAAAAGCAGGGGGAGCAGCAGAGGGAGAGGAGGAAGCAGGCTCCCCACT1740.1301012397003185No Hit
AGCAAAAGCAGGGGGAGCAGCAGAGGGAGAGGAGGAAGCAGGCTCCCCAC1710.12785811487789925No Hit
CTATGGAATGCCGCTCACTGTAAGCTTTGAACTACTGACTTGGACATCAT1650.12337186523306067No Hit
ATATAAAAGAATATACATGATTTTATTTTTGGCTCAATCAAGTTGCAGAC1620.12112874041064138No Hit
GTATATAAGCATGCAAATCCATGAATAGCTGAACCAGCAGAGGCCAATTC1570.11739019903994259No Hit
GAGCCGAAGGCAGAGGTCTAACCCACTGAGCCATCCAGGTGCCCCCAAAT1540.1151470742175233No Hit
ATCTTGGGCTAGAGATAGAATCATGAAAGTGCTTTAGAATTCAGTCATGT1500.11215624112096426No Hit
GCCTTAACAGAGACCATAAAAGAAAAGCTCAAGGCACTTGAAGACAGAAA1470.10991311629854494No Hit
GTAGATACAGAGAAATAAAGATAAAAATTACACTGGCCATTCACCAGAAA1460.10916540802440519No Hit
GTATCAGAGTCGTTTTTTTATATTTACCCTTAGTGGAATTCATTGAGCTT1410.1054268666537064No Hit
GCTCTAAGAAGCTCAATGAATTCCACTAAGGGTAAATATAAAAAAACGAC1390.10393145010542687No Hit
GCAAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGACA1380.10318374183128709No Hit
GCAAAAGCAGGAGGAGTGGGAGAGAGGGAGGCACGCTTCCTGCTAAACAA1360.10168832528300757No Hit
GAATAAGGATAAGAATATAAAAAATACGACCACAATAGGAGAACCAAAAA1350.10094061700886782No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA1250.0121.800011
AGCAAAA8200.088.4146352
GAGCAAA9050.082.513811
AAAAGCA9200.078.8043445
AGCGAAA2600.078.076931
GCAAAAG9450.076.7195743
GCGAAAG2700.075.185192
CGAAAGC2750.073.8181843
CAAAAGC10350.070.048314
AAAGCAG12400.068.9919366
GAAAGCA3550.057.1830944
ACCATCG400.006039472854.3753
GCAGGAG2850.053.421059
AAGCAGG17050.051.0263947
AGCAGGA5300.050.6132058
GCAGGAT2050.049.5121969
GCAGGGG5350.048.785059
CAAGGTA752.5386424E-548.3333364
GTGATCA1056.846312E-848.3333329
GGCATTA450.00962102248.3333329
Produced by FastQC (version 0.11.2)