FastQCFastQC Report
Wed 12 Apr 2017
000000000-ATLLR_l01n01_2016_day_8_nw.35100000088516.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-ATLLR_l01n01_2016_day_8_nw.35100000088516.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences106637
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG5970.5598432063917776No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA4960.4651293641043915No Hit
GTACTATGTTCTTTTCCATGATCGCCTGGTCCAATCGCACGCAAAGAGGG3570.33478061085739474No Hit
GAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGA3190.29914569989778406No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC2800.2625730281234468No Hit
GGCAAGTGCACCAGTTGAATAGCTTAGTGACACCTCCTTGGCCCCATGGA2260.21193394412821065No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGC2080.19505424946313193No Hit
GCGTGAACACAAATCCTAAAATTCCCTTAGTCAGAGGTGACAAGATTGGT1820.17067246828024044No Hit
GCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGCT1720.16129486013297448No Hit
ATTTAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATC1620.15191725198570852No Hit
GAGCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGCCATGG1520.1425396438384426No Hit
GAGCAAAAGCAGGTGCATAAAGATCTGGGTCCTTCTGACCTGGCTATTCA1490.13972636139426278No Hit
AGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGC1470.1378508397648096No Hit
GAGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG1460.13691307895008298No Hit
GATACTGGGAAGGGAGACACGTCAGATCCCCAGAGAGGTTTAGGGGGGAC1450.13597531813535638No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTT1420.13316203569117663No Hit
GGTCCAATCGCACGCAAAGAGGGCCTATTATCTTTTGCCTAGGCATGAGC1410.13222427487645003No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT1250.11722010184082449No Hit
GTATATACATACATATATACTTACACAGTTTGAGCATTCACAGGCCACTA1250.11722010184082449No Hit
CTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATCATCCCGTCAGGCCC1140.10690473287883193No Hit
GAGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGA1120.10502921124937872No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACTGCG100.007082041145.02
CGTTACA100.007082041145.03
GAGCAAA5800.0121.251
AGCAAAA7250.097.02
GAGCGAA1750.095.285711
AGCGAAA1800.084.5833362
GCAGGTA1550.084.193549
GCAAAAG8750.080.371433
CGAAAGC2000.076.124994
GCGAAAG2050.074.2682953
CAAAAGC9700.073.247424
AAAGCAG12700.068.503946
AAAAGCA11000.065.255
AAGCAGG13550.062.6014757
GTAGAAC350.00355777162.1428578
GCAGGTC2050.060.1219529
AGCAGGT4850.059.7938168
GCAGGGT3100.056.129039
TAGAACC400.006036124754.3759
CTGACGG400.006036124754.375145
Produced by FastQC (version 0.11.2)