FastQCFastQC Report
Wed 12 Apr 2017
000000000-ATLLR_l01n01_2016_day_8_ll-e.35100000088aa5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-ATLLR_l01n01_2016_day_8_ll-e.35100000088aa5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences141061
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGA11610.8230481848278404No Hit
GAGCAAAAGCAGGAGGAGTGGGAGAGAGGGAGGCACGCTTCCTGCTAAAC8630.6117920615903758No Hit
CAATAGGAGAACCAAAAATATGAAAGAACTGAATATAACTTCTAGGACTA6130.43456377028377796No Hit
TCTTAGAGCTATTGATGTGTTTTTTCATCTAATTTGGAAAATTTTAGCAA5720.40549833050949585No Hit
GAGCAAAAGCAGGGGGAGCAGCAGAGGGAGAGGAGGAAGCAGGCTCCCCA3680.260880044803312No Hit
GGATAAGAATATAAAAAATACGACCACAATAGGAGAACCAAAAATATGAA3640.2580443921424065No Hit
CTTAACAACTGAGCCACCCAGGTGCCCCCAAATCCCTGACTTTAACTGCA3520.24953743415968976No Hit
GAATATAACTTCTAGGACTAAAAAAATACAATATCTGAATTGGAAAAGCC3510.2488285209944634No Hit
GCAAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGACA3390.2403215630117467No Hit
GTATCTACTGTATCTTACTTTTTCCTCAGGCCACATCATGGATTTTCTTT3190.22614329970721886No Hit
GTAGATACAGAGAAATAAAGATAAAAATTACACTGGCCATTCACCAGAAA2500.17722829130659784No Hit
GAATAAGGATAAGAATATAAAAAATACGACCACAATAGGAGAACCAAAAA2430.1722658991500131No Hit
GCTCTAAGAAGCTCAATGAATTCCACTAAGGGTAAATATAAAAAAACGAC2400.17013915965433396No Hit
ATATGTATCAGAGTCGTTTTTTTATATTTACCCTTAGTGGAATTCATTGA2360.16730350699342836No Hit
GCCTTAACAGAGACCATAAAAGAAAAGCTCAAGGCACTTGAAGACAGAAA2350.166594593828202No Hit
ATGTATCAGAGTCGTTTTTTTATATTTACCCTTAGTGGAATTCATTGAGC2330.1651767674977492No Hit
GCAAAAGCAGGAGGAGTGGGAGAGAGGGAGGCACGCTTCCTGCTAAACAA2280.16163220167161724No Hit
CTTATTATGAATAAGGATAAGAATATAAAAAATACGACCACAATAGGAGA2260.16021437534116445No Hit
AGCAAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGAC2220.1573787226802589No Hit
GAGCGAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGA2170.15383415685412694No Hit
GAGTGGGAGAGAGGGAGGCACGCTTCCTGCTAAACAAGGACCCTGATTCG2120.150289591027995No Hit
TATTTATTGTCATGGTTGGGCTTGAGTTCATTTTTTTTCTGGTGAATGGC2050.14532719887141024No Hit
GATACATATCACACTTGAATTGCTGAAAATAATAATAAAGAAAATCCATG2050.14532719887141024No Hit
TTAGTGATCTGTGGAATAACATCAAGAGACCTATCATTTGGTATAACTGG1970.1396558935495991No Hit
AGATTAACCAGGGCAACTAGGTGGCTCAGTCGATTGTCTGCCTTCACCTC1950.13823806721914633No Hit
GAGCGAAAGCAGGAGGAGTGGGAGAGAGGGAGGCACGCTTCCTGCTAAAC1880.1332756750625616No Hit
GTGGAATAACATCAAGAGACCTATCATTTGGTATAACTGGAATCCAGAAG1850.13114893556688242No Hit
GGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGACAAATAGGGAA1850.13114893556688242No Hit
GGTCTAACCCACTGAGCCATCCAGGTGCCCCCAAATGTTCATTTTTTAAT1750.1240598039146185No Hit
CAGTAGATACAGAGAAATAAAGATAAAAATTACACTGGCCATTCACCAGA1720.12193306441893932No Hit
ATCTTACTTTTTCCTCAGGCCACATCATGGATTTTCTTTATTATTATTTT1710.12122415125371293No Hit
CTGTATCTACTGTATCTTACTTTTTCCTCAGGCCACATCATGGATTTTCT1710.12122415125371293No Hit
GAGCCGAAGGCAGAGGTCTAACCCACTGAGCCATCCAGGTGCCCCCAAAT1700.12051523808848655No Hit
ATATTAGGGTGACACGTGCCGTTCTGCATTTTTAAAATTAAATTCCTTAG1680.11909741175803376No Hit
GTATCAGAGTCGTTTTTTTATATTTACCCTTAGTGGAATTCATTGAGCTT1650.11697067226235458No Hit
GGTTAATCTATTTTTATTAAATATATTTATTGTCATGGTTGGGCTTGAGT1650.11697067226235458No Hit
ATATTTACCCTTAGTGGAATTCATTGAGCTTCTTAGAGCTATTGATGTGT1630.1155528459319018No Hit
AGCGAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGAC1580.11200828010576984No Hit
GTGTAATTTTTATCTTTATTTCTCTGTATCTACTGTATCTTACTTTTTCC1570.11129936694054346No Hit
GTGGGAGAGAGGGAGGCACGCTTCCTGCTAAACAAGGACCCTGATTCGGG1530.1084637142796379No Hit
AGCAAAAGCAGGAGGAGTGGGAGAGAGGGAGGCACGCTTCCTGCTAAACA1510.10704588794918511No Hit
CTTTAGTGATCTGTGGAATAACATCAAGAGACCTATCATTTGGTATAACT1510.10704588794918511No Hit
AAATTACACTGGCCATTCACCAGAAAAAAAATGAACTCAAGCCCAACCAT1440.10208349579260037No Hit
GGGTAAATATAAAAAAACGACTCTGATACATATCACACTTGAATTGCTGA1420.10066566946214758No Hit
TTATTTCTCTGTATCTACTGTATCTTACTTTTTCCTCAGGCCACATCATG1420.10066566946214758No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA1550.0116.9354861
AGCAAAA9000.078.1388852
GCAAAAG9100.077.280223
CAAAAGC9350.075.2139054
AGCGAAA2500.072.52
GCGAAAG2550.071.078433
AAAAGCA9950.070.678395
AAAGCAG12500.070.186
GAGCAAA10150.069.999991
CGAAAGC2650.065.660384
CCCCCGT350.003560157762.142853145
GATTAGC350.003560157762.1428531
AGCACAC606.77543E-660.4166685
GTCCCGG606.77543E-660.4166681
GCACACA502.0043032E-458.06
GGTATTC753.7379323E-758.0145
GCATGCA1051.0968506E-955.2380949
GAAAGCA3200.054.3755
AGCATGC953.113564E-853.4210478
AGCAGGT1650.052.7272728
Produced by FastQC (version 0.11.2)