FastQCFastQC Report
Wed 12 Apr 2017
000000000-ATLLR_l01n01_2015_day_8_ul-b.351000000888ea.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-ATLLR_l01n01_2015_day_8_ul-b.351000000888ea.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences106446
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGA10220.9601112301072844No Hit
GAGCAAAAGCAGGAGGAGTGGGAGAGAGGGAGGCACGCTTCCTGCTAAAC7940.7459181181068335No Hit
CAATAGGAGAACCAAAAATATGAAAGAACTGAATATAACTTCTAGGACTA4890.45938785863254605No Hit
TCTTAGAGCTATTGATGTGTTTTTTCATCTAATTTGGAAAATTTTAGCAA4830.453751197790429No Hit
GGATAAGAATATAAAAAATACGACCACAATAGGAGAACCAAAAATATGAA3170.2978035811585217No Hit
CTTAACAACTGAGCCACCCAGGTGCCCCCAAATCCCTGACTTTAACTGCA3090.2902880333690322No Hit
GAGCAAAAGCAGGGGGAGCAGCAGAGGGAGAGGAGGAAGCAGGCTCCCCA2940.27619638126373935No Hit
GCAAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGACA2760.25928639873738796No Hit
GTATCTACTGTATCTTACTTTTTCCTCAGGCCACATCATGGATTTTCTTT2580.24237641621103656No Hit
GAATATAACTTCTAGGACTAAAAAAATACAATATCTGAATTGGAAAAGCC2460.2311030945268023No Hit
GCCTTAACAGAGACCATAAAAGAAAAGCTCAAGGCACTTGAAGACAGAAA2030.1907070251582962No Hit
GGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGACAAATAGGGAA2020.18976758168461005No Hit
GCAAAAGCAGGAGGAGTGGGAGAGAGGGAGGCACGCTTCCTGCTAAACAA2000.18788869473723768No Hit
ATATGTATCAGAGTCGTTTTTTTATATTTACCCTTAGTGGAATTCATTGA2000.18788869473723768No Hit
ATGTATCAGAGTCGTTTTTTTATATTTACCCTTAGTGGAATTCATTGAGC1970.1850703643161791No Hit
GTAGATACAGAGAAATAAAGATAAAAATTACACTGGCCATTCACCAGAAA1920.18037314694774814No Hit
GAATAAGGATAAGAATATAAAAAATACGACCACAATAGGAGAACCAAAAA1840.17285759915825866No Hit
GAGCGAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGA1820.17097871221088626No Hit
GAGTGGGAGAGAGGGAGGCACGCTTCCTGCTAAACAAGGACCCTGATTCG1810.1700392687372001No Hit
AGCAAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGAC1760.16534205136876914No Hit
GATACATATCACACTTGAATTGCTGAAAATAATAATAAAGAAAATCCATG1720.1615842774740244No Hit
GAGCGAAAGCAGGAGGAGTGGGAGAGAGGGAGGCACGCTTCCTGCTAAAC1680.15782650357927963No Hit
GCTCTAAGAAGCTCAATGAATTCCACTAAGGGTAAATATAAAAAAACGAC1610.1512503992634763No Hit
CTGTATCTACTGTATCTTACTTTTTCCTCAGGCCACATCATGGATTTTCT1590.14937151231610393No Hit
AGCGAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGAC1560.14655318189504538No Hit
GGTTAATCTATTTTTATTAAATATATTTATTGTCATGGTTGGGCTTGAGT1540.144674294947673No Hit
TCCCAGGACCCTGGGATCATGACCTGAGGTGAAGGCAGACAATCGACTGA1490.13997707757924205No Hit
GAGCAAAAGCAGGATGAGAGACAGATTTCTGTTCCTTACGCCTTTAGAAT1470.13809819063186968No Hit
CTTATTATGAATAAGGATAAGAATATAAAAAATACGACCACAATAGGAGA1460.1371587471581835No Hit
TATTTATTGTCATGGTTGGGCTTGAGTTCATTTTTTTTCTGGTGAATGGC1450.1362193036844973No Hit
TTAGTGATCTGTGGAATAACATCAAGAGACCTATCATTTGGTATAACTGG1400.13152208631606635No Hit
GTGGAATAACATCAAGAGACCTATCATTTGGTATAACTGGAATCCAGAAG1380.12964319936869398No Hit
CAGTAGATACAGAGAAATAAAGATAAAAATTACACTGGCCATTCACCAGA1380.12964319936869398No Hit
GTATCAGAGTCGTTTTTTTATATTTACCCTTAGTGGAATTCATTGAGCTT1370.1287037558950078No Hit
GGTCTAACCCACTGAGCCATCCAGGTGCCCCCAAATGTTCATTTTTTAAT1360.1277643124213216No Hit
AGCAAAAGCAGGAGGAGTGGGAGAGAGGGAGGCACGCTTCCTGCTAAACA1360.1277643124213216No Hit
GTGGGAGAGAGGGAGGCACGCTTCCTGCTAAACAAGGACCCTGATTCGGG1300.12212765157920448No Hit
CAATAAATATATTTAATAAAAATAGATTAACCAGGGCAACTAGGTGGCTC1260.11836987768445972No Hit
ATCTTACTTTTTCCTCAGGCCACATCATGGATTTTCTTTATTATTATTTT1220.11461210378971498No Hit
TCTCTGTATCTACTGTATCTTACTTTTTCCTCAGGCCACATCATGGATTT1220.11461210378971498No Hit
ATTCAAGTGTGATATGTATCAGAGTCGTTTTTTTATATTTACCCTTAGTG1200.1127332168423426No Hit
GTATATAAGCATGCAAATCCATGAATAGCTGAACCAGCAGAGGCCAATTC1200.1127332168423426No Hit
AGATTAACCAGGGCAACTAGGTGGCTCAGTCGATTGTCTGCCTTCACCTC1190.1117937733686564No Hit
AGCGAAAGCAGGAGGAGTGGGAGAGAGGGAGGCACGCTTCCTGCTAAACA1180.11085432989497022No Hit
GAATATAAAAAATACGACCACAATAGGAGAACCAAAAATATGAAAGAACT1180.11085432989497022No Hit
TTTTTATATTTACCCTTAGTGGAATTCATTGAGCTTCTTAGAGCTATTGA1170.10991488642128402No Hit
CTACTGTATCTTACTTTTTCCTCAGGCCACATCATGGATTTTCTTTATTA1160.10897544294759785No Hit
GAGCAAAAGCAGGGACTTTTGGGCAGCATTAAGATGTATGAGGTGTATTC1150.10803599947391165No Hit
AGCAGGAGGAGTGGGAGAGAGGGAGGCACGCTTCCTGCTAAACAAGGACC1150.10803599947391165No Hit
TATTTACCCTTAGTGGAATTCATTGAGCTTCTTAGAGCTATTGATGTGTT1150.10803599947391165No Hit
CTTTAGTGATCTGTGGAATAACATCAAGAGACCTATCATTTGGTATAACT1150.10803599947391165No Hit
GTGTAATTTTTATCTTTATTTCTCTGTATCTACTGTATCTTACTTTTTCC1140.10709655600022547No Hit
CCATAAAAGAAAAGCTCAAGGCACTTGAAGACAGAAACTATCCATACAAA1120.1052176690528531No Hit
ATATTAGGGTGACACGTGCCGTTCTGCATTTTTAAAATTAAATTCCTTAG1100.10333878210548073No Hit
GCATTAAGATGTATGAGGTGTATTCCTTCCCCATCTTGGGAATTCTAGTT1090.10239933863179453No Hit
ATATTTACCCTTAGTGGAATTCATTGAGCTTCTTAGAGCTATTGATGTGT1080.10145989515810833No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGATTT100.0070820223145.06
GAGCGAA900.096.6666641
AGCAAAA6200.093.5483862
GCAGGAT502.2491804E-887.09
GCAAAAG7000.082.857153
CAAAAGC7200.079.5486154
AAAAGCA7700.074.383125
GAGCAAA7950.072.955971
AAAGCAG9500.070.2105266
AGCGAAA1800.068.472221
CGAAAGC1900.064.8684163
CATAGTA350.003557752462.1428577
GCGAAAG2000.061.6252
GATTAGC400.00603609454.3751
GAAAGCA2400.051.3541684
AAGCAGG13050.051.111117
GCAGGGG3000.048.3333369
GGGTAGA450.0096156648.3333326
ATAGCCC450.0096156648.3333325
TTTGGGC450.0096156648.3333323
Produced by FastQC (version 0.11.2)