FastQCFastQC Report
Wed 12 Apr 2017
000000000-ATLLR_l01n01_2004_day_1_ll-e.3510000008884a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-ATLLR_l01n01_2004_day_1_ll-e.3510000008884a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences109621
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGA6770.6175823975333193No Hit
GAGCAAAAGCAGGAGGAGTGGGAGAGAGGGAGGCACGCTTCCTGCTAAAC5040.4597659207633574No Hit
CAATAGGAGAACCAAAAATATGAAAGAACTGAATATAACTTCTAGGACTA3050.2782313607794127No Hit
GAGCAAAAGCAGGGGGAGCAGCAGAGGGAGAGGAGGAAGCAGGCTCCCCA2830.25816221344450424No Hit
TCTTAGAGCTATTGATGTGTTTTTTCATCTAATTTGGAAAATTTTAGCAA2650.24174200198867005No Hit
CTTAACAACTGAGCCACCCAGGTGCCCCCAAATCCCTGACTTTAACTGCA2270.20707711113746455No Hit
GAGCAAAAGCAGGCACATACCGAAATACTTAATTTCTGTCTGATTTTTTT2100.1915691336513989No Hit
GGATAAGAATATAAAAAATACGACCACAATAGGAGAACCAAAAATATGAA1980.18062232601417613No Hit
GCAAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGACA1740.15872871073973052No Hit
GGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGACAAATAGGGAA1570.1432207332536649No Hit
GAATATAACTTCTAGGACTAAAAAAATACAATATCTGAATTGGAAAAGCC1500.13683509546528494No Hit
GTATCTACTGTATCTTACTTTTTCCTCAGGCCACATCATGGATTTTCTTT1480.1350106275257478No Hit
GAGCAAAAGCAGGATGAGAGACAGATTTCTGTTCCTTACGCCTTTAGAAT1370.12497605385829358No Hit
GGTCTAACCCACTGAGCCATCCAGGTGCCCCCAAATGTTCATTTTTTAAT1350.12315158591875645No Hit
GAGCGAAAGCAGGAGGAGTGGGAGAGAGGGAGGCACGCTTCCTGCTAAAC1350.12315158591875645No Hit
ATGTATCAGAGTCGTTTTTTTATATTTACCCTTAGTGGAATTCATTGAGC1330.1213271179792193No Hit
GTAGATACAGAGAAATAAAGATAAAAATTACACTGGCCATTCACCAGAAA1270.11585371416060791No Hit
GCCTTAACAGAGACCATAAAAGAAAAGCTCAAGGCACTTGAAGACAGAAA1270.11585371416060791No Hit
CCCCACTCAGCAGGGAGCCCAATGTGGGACTCGATCCCAGGACCCTGAGA1260.11494148019083936No Hit
GAGCCGAAGGCAGAGGTCTAACCCACTGAGCCATCCAGGTGCCCCCAAAT1260.11494148019083936No Hit
ATATGTATCAGAGTCGTTTTTTTATATTTACCCTTAGTGGAATTCATTGA1260.11494148019083936No Hit
GCTCTAAGAAGCTCAATGAATTCCACTAAGGGTAAATATAAAAAAACGAC1250.11402924622107079No Hit
GATACATATCACACTTGAATTGCTGAAAATAATAATAAAGAAAATCCATG1240.11311701225130222No Hit
GAGCGAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGA1210.11038031034199651No Hit
GCAAAAGCAGGAGGAGTGGGAGAGAGGGAGGCACGCTTCCTGCTAAACAA1180.10764360843269082No Hit
ATATTAGGGTGACACGTGCCGTTCTGCATTTTTAAAATTAAATTCCTTAG1160.10581914049315369No Hit
TATTTATTGTCATGGTTGGGCTTGAGTTCATTTTTTTTCTGGTGAATGGC1150.10490690652338512No Hit
AGCGAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGAC1140.10399467255361655No Hit
CTTAACCACTCAGACACCCAGGCATCCCAACATTAAACAGATTTTTACCT1120.10217020461407943No Hit
GGATGAGAGACAGATTTCTGTTCCTTACGCCTTTAGAATAGTGATATTAG1110.10125797064431084No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA1500.0116.01
AGCAAAA6700.091.9776152
GCAAAAG7750.079.516133
CAAAAGC8350.073.80244
AGCGAAA2400.072.52
GAGCAAA8500.072.51
AAAGCAG11050.071.515846
GCGAAAG2450.071.020413
CGAAAGC2500.069.6000064
AAAAGCA9050.068.0939255
AACATCG350.003558038562.142857145
GTAGAAA1400.062.1428572
GCAGGAG2300.059.8913089
GTCTAAA502.0023213E-458.01
AGCAGGA3950.055.0632938
GTAGTGC400.006036577354.3758
AAGCAGG14850.053.70377
TCTTACC553.2063515E-452.7272729
GAAAGCA3450.050.434785
GCAGGGG3650.049.6575369
Produced by FastQC (version 0.11.2)