FastQCFastQC Report
Wed 12 Apr 2017
000000000-ATLLR_l01n01_2003_day_1_ul-b.35100000088870.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-ATLLR_l01n01_2003_day_1_ul-b.35100000088870.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences140128
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGA10090.7200559488467687No Hit
GAGCAAAAGCAGGAGGAGTGGGAGAGAGGGAGGCACGCTTCCTGCTAAAC8350.5958837634163051No Hit
CAATAGGAGAACCAAAAATATGAAAGAACTGAATATAACTTCTAGGACTA5730.4089118520210094No Hit
GAGCAAAAGCAGGGGGAGCAGCAGAGGGAGAGGAGGAAGCAGGCTCCCCA5240.37394382279059146No Hit
TCTTAGAGCTATTGATGTGTTTTTTCATCTAATTTGGAAAATTTTAGCAA4890.34896665905457863No Hit
GGATAAGAATATAAAAAATACGACCACAATAGGAGAACCAAAAATATGAA3610.2576216031057319No Hit
CTTAACAACTGAGCCACCCAGGTGCCCCCAAATCCCTGACTTTAACTGCA3020.21551724137931033No Hit
GGTCTAACCCACTGAGCCATCCAGGTGCCCCCAAATGTTCATTTTTTAAT2930.20909454213290704No Hit
GTATCTACTGTATCTTACTTTTTCCTCAGGCCACATCATGGATTTTCTTT2930.20909454213290704No Hit
GCAAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGACA2740.1955355103905001No Hit
GAATATAACTTCTAGGACTAAAAAAATACAATATCTGAATTGGAAAAGCC2570.1834037451472939No Hit
GAGCAAAAGCAGGATGAGAGACAGATTTCTGTTCCTTACGCCTTTAGAAT2530.18054921214889244No Hit
GGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGACAAATAGGGAA2340.1669901804064855No Hit
ATATTAGGGTGACACGTGCCGTTCTGCATTTTTAAAATTAAATTCCTTAG2270.16199474765928296No Hit
GCCTTAACAGAGACCATAAAAGAAAAGCTCAAGGCACTTGAAGACAGAAA2260.1612811144096826No Hit
GTAGATACAGAGAAATAAAGATAAAAATTACACTGGCCATTCACCAGAAA2220.1584265814112811No Hit
GAATAAGGATAAGAATATAAAAAATACGACCACAATAGGAGAACCAAAAA2190.15628568166248002No Hit
ATGTATCAGAGTCGTTTTTTTATATTTACCCTTAGTGGAATTCATTGAGC1990.14201301667047272No Hit
GCAAAAGCAGGAGGAGTGGGAGAGAGGGAGGCACGCTTCCTGCTAAACAA1930.1377312171728705No Hit
GAGCAAAAGCAGGCACATACCGAAATACTTAATTTCTGTCTGATTTTTTT1900.13559031742406943No Hit
ATATGTATCAGAGTCGTTTTTTTATATTTACCCTTAGTGGAATTCATTGA1870.13344941767526833No Hit
TATTTATTGTCATGGTTGGGCTTGAGTTCATTTTTTTTCTGGTGAATGGC1830.13059488467686686No Hit
GAGTGGGAGAGAGGGAGGCACGCTTCCTGCTAAACAAGGACCCTGATTCG1810.12916761817766612No Hit
CTTATTATGAATAAGGATAAGAATATAAAAAATACGACCACAATAGGAGA1810.12916761817766612No Hit
GCTCTAAGAAGCTCAATGAATTCCACTAAGGGTAAATATAAAAAAACGAC1800.12845398492806576No Hit
TTAGTGATCTGTGGAATAACATCAAGAGACCTATCATTTGGTATAACTGG1770.12631308517926468No Hit
GTGGAATAACATCAAGAGACCTATCATTTGGTATAACTGGAATCCAGAAG1760.12559945192966432No Hit
CTGTATCTACTGTATCTTACTTTTTCCTCAGGCCACATCATGGATTTTCT1760.12559945192966432No Hit
GATACATATCACACTTGAATTGCTGAAAATAATAATAAAGAAAATCCATG1740.12417218543046359No Hit
AGCAAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGAC1720.12274491893126285No Hit
AGATTAACCAGGGCAACTAGGTGGCTCAGTCGATTGTCTGCCTTCACCTC1700.1213176524320621No Hit
GTATATAAGCATGCAAATCCATGAATAGCTGAACCAGCAGAGGCCAATTC1700.1213176524320621No Hit
GAGCCGAAGGCAGAGGTCTAACCCACTGAGCCATCCAGGTGCCCCCAAAT1690.12060401918246175No Hit
GAGCGAAAGCAGGAGGAGTGGGAGAGAGGGAGGCACGCTTCCTGCTAAAC1630.11632221968485956No Hit
GAGCGAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGA1600.11418131993605847No Hit
GTATCAGAGTCGTTTTTTTATATTTACCCTTAGTGGAATTCATTGAGCTT1590.1134676866864581No Hit
AGCGAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGAC1520.10847225393925554No Hit
GGTTAATCTATTTTTATTAAATATATTTATTGTCATGGTTGGGCTTGAGT1520.10847225393925554No Hit
GTGTAATTTTTATCTTTATTTCTCTGTATCTACTGTATCTTACTTTTTCC1470.10490408769125371No Hit
GAGCGAAAGCAGGGGGAGCAGCAGAGGGAGAGGAGGAAGCAGGCTCCCCA1470.10490408769125371No Hit
ATCTTACTTTTTCCTCAGGCCACATCATGGATTTTCTTTATTATTATTTT1440.10276318794245262No Hit
GTGGGAGAGAGGGAGGCACGCTTCCTGCTAAACAAGGACCCTGATTCGGG1410.10062228819365152No Hit
TCCCAGGACCCTGGGATCATGACCTGAGGTGAAGGCAGACAATCGACTGA1410.10062228819365152No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA1750.0128.428561
AGCAAAA10000.096.4249952
GAGCAAA11050.089.230771
AAAAGCA10750.089.023265
GGTCGTT259.3697885E-487.07
AGGTCGT259.3697885E-487.06
GCAAAAG11400.084.5833363
AAAGCAG14200.083.2218256
CAAAAGC11850.080.759494
CAAGGTA554.366848E-879.090914
AGCGAAA2900.075.02
GCAGGTA406.643224E-572.59
GCAGGAG2900.072.59
GCGAAAG3050.071.311473
CGAAAGC3100.070.1612854
GCAGGTT1050.069.047629
AGCAGGT2600.066.923078
AAGCAGG18450.064.051497
AAGGTCG350.003560107262.1428575
CTAGTGT350.003560107262.1428576
Produced by FastQC (version 0.11.2)