FastQCFastQC Report
Wed 12 Apr 2017
000000000-ATLLR_l01n01_2002_day_1_ul-b.35100000088a11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-ATLLR_l01n01_2002_day_1_ul-b.35100000088a11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences141606
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAATAGGAGAACCAAAAATATGAAAGAACTGAATATAACTTCTAGGACTA4990.35238619832492973No Hit
GAGCAAAAGCAGGAGGAGTGGGAGAGAGGGAGGCACGCTTCCTGCTAAAC4240.2994223408612629No Hit
TCTTAGAGCTATTGATGTGTTTTTTCATCTAATTTGGAAAATTTTAGCAA4040.28529864553761847No Hit
GAGCAAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGA3980.2810615369405251No Hit
GAGCAAAAGCAGGGGGAGCAGCAGAGGGAGAGGAGGAAGCAGGCTCCCCA2820.19914410406338712No Hit
GTATATAAGCATGCAAATCCATGAATAGCTGAACCAGCAGAGGCCAATTC2780.19631936499865826No Hit
GGATAAGAATATAAAAAATACGACCACAATAGGAGAACCAAAAATATGAA2680.18925751733683602No Hit
GGTCTAACCCACTGAGCCATCCAGGTGCCCCCAAATGTTCATTTTTTAAT2610.18431422397356045No Hit
GTATCTACTGTATCTTACTTTTTCCTCAGGCCACATCATGGATTTTCTTT2400.16948434388373373No Hit
GAGCAAAAGCAGGATGAGAGACAGATTTCTGTTCCTTACGCCTTTAGAAT2380.16807197435136928No Hit
GCTAAACAAGGACCCTGATTCGGGGCTTGATCCCAGGACTTTGGAATCAT2160.15253590949536036No Hit
GAATATAACTTCTAGGACTAAAAAAATACAATATCTGAATTGGAAAAGCC2060.14547406183353814No Hit
ATATTAGGGTGACACGTGCCGTTCTGCATTTTTAAAATTAAATTCCTTAG2000.14123695323644478No Hit
CTTAACAACTGAGCCACCCAGGTGCCCCCAAATCCCTGACTTTAACTGCA1900.13417510557462256No Hit
GCCTTAACAGAGACCATAAAAGAAAAGCTCAAGGCACTTGAAGACAGAAA1760.1242885188480714No Hit
GTAGATACAGAGAAATAAAGATAAAAATTACACTGGCCATTCACCAGAAA1750.12358233408188918No Hit
ATATGTATCAGAGTCGTTTTTTTATATTTACCCTTAGTGGAATTCATTGA1680.11863904071861361No Hit
GAGCAAAAGCAGGGACTTTTGGGCAGCATTAAGATGTATGAGGTGTATTC1640.11581430165388473No Hit
GTCTAGTTAATAAAGCCCTACTATTCCACCGTGCTAACAGTCCCTTTGAG1620.11440193212152026No Hit
CCCTAATACTCATGCTGTCTTCAACCAAACTTCCAAAATTTTAACAAAGG1590.1122833778229736No Hit
CTATGGAATGCCGCTCACTGTAAGCTTTGAACTACTGACTTGGACATCAT1590.1122833778229736No Hit
ATGTATCAGAGTCGTTTTTTTATATTTACCCTTAGTGGAATTCATTGAGC1570.11087100829060914No Hit
TACTCATGCTGTCTTCAACCAAACTTCCAAAATTTTAACAAAGGGTTTCA1500.10592771492733358No Hit
ATGTATGAGTTACTAATTTCCTGGACATTATCAGCCATTAGCTGGTTTCC1490.10522153016115135No Hit
GAATAAGGATAAGAATATAAAAAATACGACCACAATAGGAGAACCAAAAA1440.10169060633024025No Hit
GTTAATATACTTAATACCATTGAATTATACATAGACAAGTGGTTGGATTA1440.10169060633024025No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGAAA151.2277938E-4145.08
CCGAAAT151.2277938E-4145.09
ATACCGA151.2277938E-4145.06
TACCGAA203.8591315E-4108.757
AGCAAAA7550.087.38412
CATACCG259.369995E-487.05
GAGCGAA1450.085.01
GCGAAAG2150.084.302322
AGCGAAA2200.082.386371
GAGCAAA8300.080.361441
GCAAAAG8400.079.404763
AAAAGCA9100.074.8901145
CGAAAGC2450.073.979593
CAAAAGC9550.071.361254
AGCAGGT1850.070.5405358
AAAGCAG11900.067.016816
GCAGGAT1550.065.483879
ACATACC350.003560185462.1428574
GCAGGTG753.7380232E-758.0000049
GCAGGTA400.006040206654.3759
Produced by FastQC (version 0.11.2)