FastQCFastQC Report
Wed 12 Apr 2017
000000000-ATLLR_l01n01_2002_day_1_ll-e.35100000088999.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-ATLLR_l01n01_2002_day_1_ll-e.35100000088999.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43533
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGA2550.5857625249810489No Hit
GAGCAAAAGCAGGAGGAGTGGGAGAGAGGGAGGCACGCTTCCTGCTAAAC2440.5604943376289252No Hit
GAGCAAAAGCAGGGGGAGCAGCAGAGGGAGAGGAGGAAGCAGGCTCCCCA2150.49387820733696275No Hit
GAGCAAAAGCAGGATGAGAGACAGATTTCTGTTCCTTACGCCTTTAGAAT1530.3514575149886293No Hit
TCTTAGAGCTATTGATGTGTTTTTTCATCTAATTTGGAAAATTTTAGCAA1350.3101095720487906No Hit
CAATAGGAGAACCAAAAATATGAAAGAACTGAATATAACTTCTAGGACTA1270.29173270851997335No Hit
CTTAACAACTGAGCCACCCAGGTGCCCCCAAATCCCTGACTTTAACTGCA1160.2664645211678497No Hit
GGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGACAAATAGGGAA1120.25727608940344104No Hit
ATATTAGGGTGACACGTGCCGTTCTGCATTTTTAAAATTAAATTCCTTAG1100.2526818735212368No Hit
GAGCCGAAGGCAGAGGTCTAACCCACTGAGCCATCCAGGTGCCCCCAAAT1090.2503847655801346No Hit
GGTCTAACCCACTGAGCCATCCAGGTGCCCCCAAATGTTCATTTTTTAAT1030.2366021179335217No Hit
CCCCACTCAGCAGGGAGCCCAATGTGGGACTCGATCCCAGGACCCTGAGA880.20214549881698943No Hit
GAGGTCTAACCCACTGAGCCATCCAGGTGCCCCCAAATGTTCATTTTTTA820.1883628511703765No Hit
GAATATAACTTCTAGGACTAAAAAAATACAATATCTGAATTGGAAAAGCC790.18147152734707003No Hit
GAGCAAAAGCAGGCACATACCGAAATACTTAATTTCTGTCTGATTTTTTT780.17917441940596787No Hit
GCAAAAGCAGGAGGAGTGGGAGAGAGGGAGGCACGCTTCCTGCTAAACAA740.16998598764155928No Hit
AGCAAAAGCAGGGGGAGCAGCAGAGGGAGAGGAGGAAGCAGGCTCCCCAC730.16768887970045712No Hit
GCAAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGACA720.16539177175935496No Hit
GGATAAGAATATAAAAAATACGACCACAATAGGAGAACCAAAAATATGAA700.16079755587715067No Hit
ATATGTATCAGAGTCGTTTTTTTATATTTACCCTTAGTGGAATTCATTGA690.15850044793604853No Hit
GCTAAACAAGGACCCTGATTCGGGGCTTGATCCCAGGACTTTGGAATCAT650.14931201617163992No Hit
GAGCGAAAGCAGGAGGAGTGGGAGAGAGGGAGGCACGCTTCCTGCTAAAC650.14931201617163992No Hit
CTTAACCACTCAGACACCCAGGCATCCCAACATTAAACAGATTTTTACCT640.14701490823053776No Hit
GCAAAAGCAGGGGGAGCAGCAGAGGGAGAGGAGGAAGCAGGCTCCCCACT640.14701490823053776No Hit
TCCCAGGACCCTGCGATCATGAACTGAGTCAAAGGCAGACACTTAACCAC630.1447178002894356No Hit
TTTCTGTTCCTTACGCCTTTAGAATAGTGATATTAGGGTGACACGTGCCG610.1401235844072313No Hit
GTAGATACAGAGAAATAAAGATAAAAATTACACTGGCCATTCACCAGAAA610.1401235844072313No Hit
GAGTGGGAGAGAGGGAGGCACGCTTCCTGCTAAACAAGGACCCTGATTCG600.13782647646612914No Hit
GACAATCGACTGAGCCACCTAGTTGCCCTGGTTAATCTATTTTTATTAAA600.13782647646612914No Hit
GTATCTACTGTATCTTACTTTTTCCTCAGGCCACATCATGGATTTTCTTT590.135529368525027No Hit
ACCCTGAGATCATGACCTGAGCCGAAGGCAGAGGTCTAACCCACTGAGCC590.135529368525027No Hit
GCCCAATGTGGGACTCGATCCCAGGACCCTGAGATCATGACCTGAGCCGA590.135529368525027No Hit
GAGCGAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGA560.12863804470172052No Hit
GAATAAGGATAAGAATATAAAAAATACGACCACAATAGGAGAACCAAAAA560.12863804470172052No Hit
GGATGAGAGACAGATTTCTGTTCCTTACGCCTTTAGAATAGTGATATTAG530.1217467208784141No Hit
GAGCAAAAGCAGGGATGTGCACAGATACTTGTATACCCATGTTCATAGCA530.1217467208784141No Hit
GCCTTAACAGAGACCATAAAAGAAAAGCTCAAGGCACTTGAAGACAGAAA530.1217467208784141No Hit
GCAAAAGCAGGATGAGAGACAGATTTCTGTTCCTTACGCCTTTAGAATAG520.11944961293731192No Hit
AACCCACTGAGCCATCCAGGTGCCCCCAAATGTTCATTTTTTAATACATC500.11485539705510762No Hit
CCTTTAGAATAGTGATATTAGGGTGACACGTGCCGTTCTGCATTTTTAAA500.11485539705510762No Hit
CTTGTATACCCATGTTCATAGCAGCATTCCTCCAAATAGCTAAAAGGTTA490.11255828911400548No Hit
ATCTTGGGCTAGAGATAGAATCATGAAAGTGCTTTAGAATTCAGTCATGT490.11255828911400548No Hit
GTAATATTTAAAATGTAGGTAAATCTACAGTCTATCTTGGGCTAGAGATA480.11026118117290333No Hit
GTATATAAGCATGCAAATCCATGAATAGCTGAACCAGCAGAGGCCAATTC480.11026118117290333No Hit
AGCAAAAGCAGGAGGAGTGGGAGAGAGGGAGGCACGCTTCCTGCTAAACA470.10796407323180116No Hit
TATTTATTGTCATGGTTGGGCTTGAGTTCATTTTTTTTCTGGTGAATGGC470.10796407323180116No Hit
ATGTATCAGAGTCGTTTTTTTATATTTACCCTTAGTGGAATTCATTGAGC470.10796407323180116No Hit
CAGTAGATACAGAGAAATAAAGATAAAAATTACACTGGCCATTCACCAGA470.10796407323180116No Hit
AGCGAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGAC460.10566696529069901No Hit
CTGATACATATCACACTTGAATTGCTGAAAATAATAATAAAGAAAATCCA460.10566696529069901No Hit
GTATCAGAGTCGTTTTTTTATATTTACCCTTAGTGGAATTCATTGAGCTT450.10336985734959686No Hit
GGTTAATCTATTTTTATTAAATATATTTATTGTCATGGTTGGGCTTGAGT450.10336985734959686No Hit
GTACTATATGACACTACTTACATAAGGTACTTAGAGTAATCAGATTCCTA450.10336985734959686No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTCGCT100.007067731144.999987
AGCAAAA2750.0110.7272642
GAGCGAA404.7075446E-9108.749991
GCAAAAG3300.092.272733
GAGCAAA3350.090.895521
GGGTCAA259.32607E-487.01
AGCAGGT353.4003744E-582.857148
AAAAGCA3700.082.29735
AAAGCAG4350.080.06
AGCGAAA554.296089E-879.0909042
CAAAAGC4150.073.37354
AAGAGTA300.001923275872.55
GAGTAAA300.001923275872.57
GCAGGGG2100.069.0476159
CGAAAGC651.3668796E-766.923084
GCGAAAG651.3668796E-766.923083
TGCACAG350.003543619862.1428575
GCAGGAG851.2667442E-859.705889
TATTATC400.006012205554.3749962
GTAAATT400.006012205554.3749969
Produced by FastQC (version 0.11.2)