FastQCFastQC Report
Thu 16 Mar 2017
000000000-ATLL5_l01n03.33300000080da7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-ATLL5_l01n03.33300000080da7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18001004
Sequences flagged as poor quality0
Sequence length251
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTTGCTCCCCACGCTTTCGCACATGAGCGTCAGTACATTCCCAAGG201995111.221324099478007No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTCGTCCAGGG195624610.867427172395496No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTATTGGCCCAGGG186322210.350655996743292No Hit
CCTGTTCGCTACCCACGCTTTCGAGCCTCAGCGTCAGTTACAAGCCAGAG9290055.160851028087101No Hit
CCTGTTTGATACCCACGCTTTCGTGCTTCAGCGTCAGTTGTACCTTAGTA5731903.184211280659679No Hit
CCTGTTTGCTACCCACGCTTTCGAGCATGAACGTCAGTGTTATCCCAGGA5388922.993677463768132No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAGCGTCAGTTGCAGCCCGGAC4354462.419009517469137No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGTGTCAGTTACAGGCCAAAA3752492.0846003922892304No Hit
CCCGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA3419731.8997440364992975No Hit
CCTGTTTGCTACCCACGCTTTCGCGCTTCAGCGTCAGTATCTGTCCAGTA2859591.5885725040669954No Hit
CCTGTTTGCTACCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG2602411.4457026952496648No Hit
CCTGTTTGCTACCCACGCTTTCGAACCTCAGTGTCAGTATGATGCCAGGA2258341.2545633565772218No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAAGCCAGAG2023021.1238373148519938No Hit
CCTGTTTGCTACCCACGCTTTCGCGCTTTAGCGTCAGTATCTGTCCAGTA1753770.9742623244792347No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCATGAGCGTCAGTATTGGCCCAGGG1077810.5987499363924368No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG954390.5301870940087564No Hit
CCTGTTCGCTCCCCCGGCTTTCGCACTTCAGCGTCAGTTACCGTCCAGTG855160.47506239096441505No Hit
CCTGTTTGCTCCCCACGCTTTCGCACATGAGCGTCAGTACTTTCCCAAGG704770.39151705093782546No Hit
CCTGTTTGCTCCCCAAACTGTCGTCCCTCATCGTCAAGTATTCTATAGTT686970.38162871359841927No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTGAGCGTCAGTCTTCGTCCAGGG629000.34942495429699366No Hit
CCTGTTTGCTCCCCACGCTTTCGCACATGAGCGTCAGTACATTCCCAGGG620970.3449640920028683No Hit
CCTGTTTGCTCCCCTAGCTTTCGCACTTCAGCGTCAGTTTCTGTCCAGTG603180.3350813099091584No Hit
CCTGTTCGCTACCCACGCTTTCGCGCCTCAGCGTCAGTTACAAGCCAGAG583150.3239541527794783No Hit
CCTGTTTGCTCCCCACGCCTTCGAGCCTCAACGTCAGTTATCGTCCAGTA513690.28536741617300904No Hit
CCTGTTTGCTACCCACGCTTTCGGGCATGAACGTCAGTGTTATCCCAGGA485330.26961273937831465No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGCGTCAGTTGTTGTCCAGAA461170.2561912657760645No Hit
CCTTTTCGCTCCCCACGCTTTCGTTTTTCAGCGTCAATACAGTTCCAGTA459160.25507466139110907No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCATGAGCGTCAGTACTTTCCCAGGG401180.22286534684398718No Hit
CCTTCTCGCTACCCAAGCCTTCGTCCCTCAACGTCAGTTTTTACATAGAA398130.22117099690661698No Hit
CCTGTTTGCTCCCCTAGCTTTCGCACTTCAGCGTCAGTTACCGTCCAGTG385560.2141880530663734No Hit
CCTGTTTGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGACCCAGAG377880.20992162437161838No Hit
CCTGTTTGCTCCCCACGCTTTCGCACATGAGCGTCAGTACTTTCCCAGGG367850.2043497129382339No Hit
CCTGTTTGCTACCCACGCTTTCGCGCATGAACGTCAGTGTTATCCCAGGA366980.20386640656265617No Hit
CCTGTTCGCTCCCCACGCTTTCGTCCATCAGCGTCAATCAATTGTTAGTA336150.18673958408097682No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTGAGCGTCAGTATTGGCCCAGGG329570.1830842324128143No Hit
CCTGTTTGATACCCACACTTTCGCGCCTCAGCGTCAGTTGCAGCCCGGAC325910.18105101248797012No Hit
CCTGTTCGCTCCCCTAGCTTTCGCACTTCAGCGTCAGTTGCCGTCCAGTG323060.17946776746452586No Hit
CCCGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTTACCGTCCAGAA307390.17076269745843065No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTATTGTCCCAGGG266210.14788619568108533No Hit
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAAGCCAGAG261730.14539744560914492No Hit
CCTGTTCGCTCCCCACGCTTTCGTCCCTCAGCGTCAGTTGTATCTTAGTA257180.1428698088173304No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTGTTATCCCAGGA250440.13912557321802718No Hit
CCTGTTTGCTACCCACGCTTTCGTGCTTCAGCGTCAGTTGTACCTTAGTA241930.1343980591304796No Hit
CCTGTTTGCTACCCACGCTTTCGAGCCTCAGCGTCAGTTACAAGCCAGAG239720.13317034983159828No Hit
CCTGTTCGCTCCCCATGCTTTCGCTCCTCAGCGTCAGTTACTGCCCAGAG222800.12377087411346611No Hit
CCCGTTCGCTCCCCTGGCTTTCGAGCCTCAGCGTCAGTTGCAGTCCAGAA222780.12375976362207351No Hit
CCTGTTTGCTCCCCATGCTTTCGTATCTCAGTGTCAGTATCAGGCCAGAA212460.11802675006349644No Hit
CCTGTTCGCTCCCCTAGCTTTCGCACTTCAGCGTCAGTTACCGTCCAGTG206640.1147935970682524No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCATGAGCGTCAGTATTGTCCCAGGG202920.11272704566923045No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCATGAGCGTCAGTACATTCCCAAGG201740.11207152667706756No Hit
CCTGTTTGCTACCCACGCTTTCGCACCTCAGCGTCAGTGTCAGTCCAGAA195940.10884948417321612No Hit
ACTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTCGTCCAGGG195890.10882170794473463No Hit
CCTGTTCGCTCCCCCAGCTTTCGCACTTCAGCGTCAGTTACCGTCCAGTG193370.10742178602926813No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGCGCGTCAGTATTGGCCCAGGG193110.10727734964116446No Hit
CCTGTTTGCTACCCACGCTTTCGAGCATGAACGTCAGTATTATCCCAGGG191090.10615519001051275No Hit
CCTGTTCGATACCCGCACCTTCGAGCTTAAGCGTCAGTTGCGCTCCCGCC183580.10198320049259475No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCATGAGCGTCAGTATTTTCCCAGGG182530.10139989969448371No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTTTGC12357950.0237.625953
TTTGCTC9822250.0237.557345
CTGTTTG13856750.0237.263632
GTTTGCT12378500.0237.128514
TTGATAC1433350.0236.982256
CTGTTCG2095300.0236.34952
TGTTTGA1536950.0236.061233
CCTGTTT13819550.0235.910231
TTGCTCC9935700.0235.602226
TTTGCTA2589050.0234.714375
CCTGTTC2095950.0234.378711
TGCTCCC9977350.0234.234427
GATACCC1577600.0233.79788
GTTTGAT1553200.0233.077854
TGATACC1454250.0232.937777
TTGCTAC2621100.0232.798346
CTCCCCA10222350.0232.578579
GCTCCCC11289800.0232.388768
TGTTCGC2000000.0232.138053
TGTTCGA140100.0231.940933
Produced by FastQC (version 0.11.2)