FastQCFastQC Report
Thu 16 Mar 2017
000000000-ATLL5_l01n02.33200000080daa.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-ATLL5_l01n02.33200000080daa.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18001004
Sequences flagged as poor quality0
Sequence length12
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGCTCGTAACT12752117.084110419618817No Hit
GTCTACACACAT7670234.261001219709745No Hit
AAGAGATGTCGA3925262.1805783721841294No Hit
ACGGGACATGCT3207091.7816172920132676No Hit
CACGCCATAATG2896321.608976921509489No Hit
TCCAAAGTGTTC2859881.588733606192188No Hit
GCTCAGTGCAGA2352441.3068382185793637No Hit
ATTCCTGTGAGT2309201.2828173361885815No Hit
AATCAGTCTCGT1956631.0869560386742871No Hit
AATGGAGCATGA1877681.0430973739020335No Hit
AGCTTGACAGCT1817671.0097603444785634No Hit
ACGTGTACCCAA1767720.9820118922255671No Hit
GACGGAACCCAT1766760.9814785886387225No Hit
CTAACCTCCGCT1706590.9480526752841119No Hit
GTTGGTCAATCT1706090.9477749129992972No Hit
CCTGAACTAGTT1675300.930670311500403No Hit
GAATCTTCGAGC1625650.9030885166182954No Hit
AATCCGTACAGC1619670.8997664796919105No Hit
CAGCGGTGACAT1598960.8882615658548823No Hit
ACACGTAAGCCT1574930.8749123104466839No Hit
GACTTTCCCTCG1540870.8559911436051011No Hit
GTCAATTGACCG1527350.8484804514237094No Hit
TGTAATTGTCGC1521030.8449695361436506No Hit
GCGATATATCGC1501800.8342867986696743No Hit
ACGCGCAGATAC1501800.8342867986696743No Hit
TCGTCGATAATC1472480.8179988182881355No Hit
CACGGTTGTGAG1462260.8123213571865214No Hit
AGGCATCTTACG1456950.8093715217217884No Hit
CGGAGCTATGGT1455170.8083826879878477No Hit
ATGTGCACGACT1454040.8077549452241664No Hit
GAGGCTCATCAT1450170.8056050651396999No Hit
GTCATATCGTAC1441570.8008275538408858No Hit
GACAGGAGATAG1423760.7909336612557832No Hit
TCCCAGAACAAC1408620.7825230192715917No Hit
TTACTGTGCGAT1401400.7785121318788663No Hit
TGGCAAGACTCT1392160.7733790848554892No Hit
AATCTTGCTGCA1381640.7675349663829862No Hit
ATGAGACTCCAC1377480.7652239841733273No Hit
GAGGAATAGCAG1376820.7648573379573717No Hit
TCCTCTGTCGAC1364980.7582799270529578No Hit
TATCAGGTGTGC1341660.7453250940891964No Hit
GGAGACAAGGGA1339670.7442196001956335No Hit
ACACCTGGTGAT1331530.7396976301988489No Hit
CAAGCATGCCTA1331380.7396143015134045No Hit
CAGGCGTATTGG1329970.7388310118702268No Hit
CAGCTAGAACGC1319950.7332646556825386No Hit
TACTAATCTGCG1317200.7317369631160573No Hit
GAGTGGTAGAGA1297440.7207597976201772No Hit
TACACGATCTAC1282560.7124935920240892No Hit
TGTTATCGCACA1241550.689711529423581No Hit
CCTCGTTCGACT1236000.686628368062137No Hit
GAACTAGTCACC1226170.6811675615426784No Hit
AGCTGGAAGTCC1215080.6750067940654866No Hit
TCTAGCGTAGTG1205190.6695126560718502No Hit
TCGAGGACTGCA1200750.6670461269826949No Hit
GGCCACGTAGTA1190730.6614797707950067No Hit
TGAGTCACTGGT1188650.6603242796901773No Hit
AGGCTACACGAC1171070.6505581577560896No Hit
GAACCAAAGGAT1163180.6461750689017123No Hit
ATCCCGAATTTG1162930.6460361877593049No Hit
GACTTGGTATTC1136130.6311481292932327No Hit
TATCGTTGACCA1130530.6280371917033072No Hit
ACTCACGGTATG1116960.620498723293434No Hit
CTAGCGAACATC1095160.6083882876755096No Hit
TAGGATTGCTCG1087030.6038718729244213No Hit
GAAGTTGGAAGT1085750.6031608014752955No Hit
CCAGTGTATGCA1074540.5969333710497482No Hit
TACAGATGGCTC1064780.5915114512501636No Hit
CCATGCGATAAC1031280.5729013781675734No Hit
GCACACACGTTA1027110.570584840712218No Hit
CTTCGGCAGAAT979760.5442807523402583No Hit
CAACACGCACGA943800.5243040888163794No Hit
TAATCCACAGCG939950.5221653192233056No Hit
TAGGAACTGGCC887240.4928836191581314No Hit
TCAAGCTCAAGC855420.4752068273525188No Hit
CGACTGTCTTAA844780.46929604593166024No Hit
ATCGCTCGAGGA822100.45669674869246185No Hit
TTCCTAGGTGAG805640.4475528142763593No Hit
CCTCTCGTGATC746860.41489908007353365No Hit
GTATGCGCTGTA724940.4027219815072537No Hit
GCATGGCTCTAA683140.3795010544967381No Hit
TCTTCCGCTACT630310.3501526914832084No Hit
CCACCTACTCCA565270.31402137347450176No Hit
GTACCTAATTGC521400.289650510604853No Hit
GATTGGTTGCAC501150.27840113806985434No Hit
GAAGAAGCGGTA485240.269562742167048No Hit
GTCTAATTCCGA467110.25949108171966406No Hit
TCCGACACAATT419820.23322032482188215No Hit
CGATCCGTATTA412340.22906500104105304No Hit
AGTAGAGGGATG335380.18631183016236205No Hit
ATTGGGCTAGGC335020.1861118413172954No Hit
TCACGGGAGTTG310690.1725959285382082No Hit
TGCAATGTTGCT242600.1347702605921314No Hit
CGCAGCGGTATA230180.12787064543733226No Hit
CAGTGCATATGC223290.1240430811525846No Hit
TAGCTCGTACCT188980.10498303316859438No Hit
TAGCTCGTAGCT185550.10307758389476498No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGACCA1101.815497E-76.00000054
CCTGTGG5500.06.00000054
GCGTTAC806.539645E-56.00000055
TAGCCGT550.00916088656.00000051
GGCCCCG550.00916088656.00000051
CGGGCCA1750.06.00000052
ACCCGAT806.539645E-56.00000052
CACCGTG550.00916088656.00000053
CTCAGTA806.539645E-56.00000052
TATCCGG1750.06.00000051
GAGTGGC600.00339664546.01
TGCCACG1900.06.01
ACCGATG650.00126200516.02
CCAGATG650.00126200516.02
TCAGGGC1900.06.03
CGAGGAT650.00126200516.02
ATCCCGG600.00339664546.01
GGTGCCA852.445087E-56.05
GGGCCAT1800.06.03
CCGGTTG852.445087E-56.02
Produced by FastQC (version 0.11.2)