FastQCFastQC Report
Thu 16 Mar 2017
000000000-ATLL5_l01n01.33100000080dad.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-ATLL5_l01n01.33100000080dad.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18001004
Sequences flagged as poor quality0
Sequence length251
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA216011611.99997511249928No Hit
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCA207831811.545567125033692No Hit
TACGGGGGGTGCGAGCGTTAATCGGAATAACTGGGCGTAAAGGGCACGCA11741436.522652847585612No Hit
TACGTAGGTCCCGAGCGTTATCCGGATTTATTGGGCGTAAAGCGAGCGCA10639545.910525879556496No Hit
TACGGAGGGTGCGAGCGTTAATCGGAATAACTGGGCGTAAAGGGCACGCA10262115.700854241241211No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCGCGCA8010504.450029565017596No Hit
TACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGAGCGCA6974493.8745005556356746No Hit
TACGGAGGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA6042103.3565350021587683No Hit
TACGGAAGGTCCAGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA4776592.6535131040468634No Hit
TACGTATGTCACAAGCGTTATCCGGATTTATTGGGCGTAAAGCGCGTCTA4221662.345235854622331No Hit
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA3507371.9484302097816324No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA3001111.6671903411609708No Hit
TACAGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGAGCGTA2523511.4018718067058926No Hit
TACGTATGTCGCGAGCGTTATCCGGAATTATTGGGCATAAAGGGCATCTA1371270.7617741765959277No Hit
TACGGGGGGTGCAAGCGTTAATCGGAATAACTGGGCGTAAAGGGCACGCA1170100.6500192989235489No Hit
TACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGCA1092530.6069272580573839No Hit
TACGTATGTCGCAAGCGTTATCCGGAATTATTGGGCATAAAGGGCATCTA1010200.5611909202397821No Hit
TACGTAGGTCTCAAGCGTTATCCGGATTTACTGGGCGTAAAGTGTCCGTA799650.4442252221042782No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA726960.40384414113790545No Hit
TACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGGGCGCA697640.3875561607563667No Hit
TACGTATGTCGCAAGCGTTATCCGGAATTATTGGGCTTAAAGGGCATCTA627510.3485972226882456No Hit
TACGTAGGGCGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTCGTA507290.2818120589273798No Hit
TACGGAAGGTCCGGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA500040.27778450579756553No Hit
TACGGAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCA478550.26584628279622624No Hit
TACATATGGGGCGAGCGTTATCCGGAATTATTGGGTGTAAAGGGTGCGTA448170.2489694463708802No Hit
TACGGAGGATGCGAGCGTTATCCGGAATCATTGGGTTTAAAGGGTCCGTA413810.2298816221584085No Hit
TACGGAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGCA404580.22475413038072767No Hit
TACGTAGAAGACTAGTGTTAATCATCTTTATTAGGTTTAAAGGGTACCTA402810.22377085189248333No Hit
TACGGAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA391220.2173323221304767No Hit
TACGGAGGATGCGAGCGTTATCCGGAATCATTGGGTTTAAAGGGTCTGTA359570.19974996950170112No Hit
TACGGAGGATGCGAGCGTTATCCGGATTTATTGGGCTTAAAGGGTGCGTA312180.17342366014695623No Hit
TACATATGTCTCGAGCGTTACCCGGAATCACTGGGTGTAAAGGGTTTGTA308870.1715848738214824No Hit
TACGTAGGGTGCGAGCGTTGTCCGGAATTACTGGGCGTAAAGAGCTCGTA292440.1624576051424687No Hit
TACGTAGGTAGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCGCGCA288700.16037994325205418No Hit
TACATATGTCTCAAGCGTTACCCGGAATCACTGGGTGTAAAGGGTTTGTA279120.15505801787500298No Hit
TACGTAGGTGGCAAGCGTTATCCGGAATTATTGGGCGTAAAGCGCGCGTA276580.15364698546814387No Hit
TACGGAGGGTGCGAGCGTTAATCGGAATAACTGGGCGTAAAGCGCACGCA275100.15282480910509214No Hit
TACGTAGGTGGCGAGCGTTGTCCGGAATCATTGGGCGTAAAGGGAGCGCA257860.14324756552467852No Hit
TACGTAGGTGGCGAGCGTTGTCCGGATTTATTGGGCGTAAAGGGAGTGTA257160.1428586983259378No Hit
TACAGGGGGTGCGAGCGTTAATCGGAATGACTGGGCGTAAAGGGTATGTA247680.1375923254058496No Hit
TACGGAGGGTGCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTCCGTA239160.13285925607260574No Hit
TACGGAAGGTTCGGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA236190.13120934810080595No Hit
TACGGAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGAGCGCA234350.13018718289268755No Hit
TACGTAGGTCCCGAGCGTTATCCGGATTTATTGGGCGTAAAGCGCGCGCA225260.12513746455475483No Hit
TACGGAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGTA222910.12383198181612537No Hit
TACGGAAGGTCCAGGCGTTATCCGGATTTATTGGGCTTAAAGGGAGCGTA222910.12383198181612537No Hit
TACGTAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA210360.11686014846727438No Hit
TACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGAGTGCGCA206960.11497136493053388No Hit
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGCA203620.11311591286797115No Hit
TACGTAGGGTGCGAGCGTTAATCGGCATTACTGGGCGTAAAGCGTGCGCA191950.10663294114039418No Hit
TACGTATGTCACGAGCGTTATCCGGATTTATTGGGCGTAAAGCGCGTCTA182690.10148878362562443No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTAT952950.0259.486541
TACGTAG7226150.0259.19441
TACGGAG4911100.0259.11521
TACAGAG330700.0256.390561
TACGGGG1534250.0250.814221
ACGTAGG7387900.0250.644852
ACGGAGG5077050.0249.836582
CGTATGT974150.0247.909423
ACGTATG998100.0247.61832
ACATATG151900.0247.166822
TACGGAA790050.0245.66791
TAGGTCC1719150.0244.616465
GGTCCCG1722700.0244.311397
AGGTCCC1738450.0244.26026
GTCCCGA1725250.0244.014148
TCACAAG550450.0243.612099
GTCACAA551350.0243.303278
GTAGGGT3776600.0243.301094
TATGTCG403200.0243.179415
GTAGGTC1908850.0242.532334
Produced by FastQC (version 0.11.2)