FastQCFastQC Report
Wed 5 Jul 2017
000000000-ARRUV_l01n03.33300000093a29.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-ARRUV_l01n03.33300000093a29.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11901668
Sequences flagged as poor quality0
Sequence length251
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTCGCTACCCATGCTTTCGCTCCTCAGCGTCAGTAACTGCCCAGTA6410425.38615259642598No Hit
CCTGTTTGATCCCCACGCTTTCGCACATCAGCGTCAGTTACAGACCAGAA5728464.813157281819658No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTATTGGCCCAGGG4463163.750028987533512No Hit
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAATGTTAGACCAGAA2044431.717767627193096No Hit
CCTGTTTGCTACCCATGCTTTCGCTCCTCAGCGTCAGTAACTGCCCAGTA1668171.4016270660549428No Hit
CCTGTTTGCTACCCACGCTTTCGCACCTCAGTGTCAGTATGATGCCAGGG1653921.3896539543868978No Hit
CCTGTTCGCTACCCATGCTTTCGCTCCTCAGCGTCAGTTACTACCCAGAG1647211.3840160891733833No Hit
CCTGTTTGCTACCCACGCTTTCGTTCCTCAGCGTCAGAATGAGCCCAGTA883180.7420640535427472No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTGTTATCCCAGGA769790.6467916933996143No Hit
CCTGTTTGCTACCCACGCTTTCGTACCTCAGCGTCAGATAATGGCCAGAA646410.5431255518134097No Hit
CCTGTTTGCTACCCACGCTTTCGTACCTCAGCGTCAGTTAATGGCCAGAA643880.5409997993558551No Hit
CCTGTTCGCTACCCATGCTCTCGCTCCTCAGCGTCAGTAACTGCCCAGTA598120.5025514070800832No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAAGCCAGAG564630.47441249411427033No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTTGTCCAGGG421800.35440410537413747No Hit
CCTGTTTGCTCCCCACGCTCTCGTGCATGAGCGTCAGTATTGGCCCAGGG408870.34354008194481644No Hit
CCTGTTTGCTCCCCACGCTTTCGTACCTCAGCGTCAGTTTGTGTCCAGAA396460.3331129720640838No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGTGTCAGTATCAGTCCAGGT391030.32855058635478657No Hit
CCTGTTTGATCCCCACGCTCTCGCACATCAGCGTCAGTTACAGACCAGAA366670.3080828670401493No Hit
CCTGTTTGCTCCCCACGCTTTCGCACATCAGCGTCAGTTACAGACCAGAA354400.2977733877301904No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTCGTCCAGGG279040.23445453191939147No Hit
CCTGTTCGCTACCCATGCTTTCGCTCCTCAGCGTCAGTTACTGCCCAGAG262650.22068335295523284No Hit
CCTGTTCGCTCCCCACGCTTTCGCGCCTCAGCGTCAATGTTAGACCAGAA259240.2178182083385287No Hit
CCTGTTCGCTACCCACGCTTTCGCTCCTCAGCGTCAGTAACTGCCCAGTA259050.2176585668496214No Hit
CCCGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA243340.20445873637207826No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG236100.19837555542634866No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCATGAGCGTCAGTATTGGCCCAGGG216660.182041710456047No Hit
CCTGTTTGCTCCCCACGCTTTCGCACATGAGCGTCAGTACATTCCCAAGG205500.17266487352865162No Hit
CCTGTTTGCTACCCACGCTCTCGCACCTCAGTGTCAGTATGATGCCAGGG175330.1473154855269026No Hit
CCTGTTCGCTCCCCACGCTCTCGAGCCTCAGCGTCAATGTTAGACCAGAA171310.14393780770896986No Hit
CCCATTCGCTCCCCTAGCTTTCGTCTCTCAGTGTCAGTGTCGGCCCAGCA158920.1335275021954906No Hit
CCTGTTTGCTACCCACGCTTTCGCACCTCAGCGTCAGTGTCAGTCCAGAA155910.13099844492385437No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTGAGCGTCAGTATTGGCCCAGGG155240.13043549862086556No Hit
CGTTTTCGATACCCTAACCTTCGTTCTTGATTAATGAAAACATCCTTGGC143710.12074778089928234No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACAGACCAGAG143090.12022684551442706No Hit
CCTGTTTGCTACCCATGCTCTCGCTCCTCAGCGTCAGTAACTGCCCAGTA142050.11935301841725042No Hit
CCTGTTCGCTCCCCATGCTTTCGCTCCTCAGCGTCAGTAACTGCCCAGTA139490.11720205940881565No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTTACAGACCAGAG138840.11665591747308025No Hit
CCTGTTCGCTACCCATGCTCTCGCTCCTCAGCGTCAGTTACTACCCAGAG135750.11405964273243044No Hit
CCTGTTTGATCCCCACGCTTTCGCACCTCAGCGTCAGTTACAGACCAGAA120680.101397552006996No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTTTGC2497950.0444.48093
GTTTGCT2520450.0443.521154
TTTGCTC1596150.0442.363925
TTTGATC835050.0442.355265
TGTTTGA872650.0440.77473
TGTTCGC1943250.0439.875153
TTTGCTA960000.0437.43215
GTTCGCT2026200.0433.942964
TTGCTAC993650.0433.80516
GTTTGAT890750.0432.54634
TGATCCC871650.0431.889257
GATCCCC883350.0431.131388
TTGATCC877350.0429.72066
TTGCTCC1689400.0427.094646
ATCCCCA882000.0423.67689
TGCTCCC1732250.0416.65897
CTCCCCA2448800.0402.785589
TTGATAC43250.0400.25216
TTCGCTA1475500.0393.52995
TCGCTAC1515550.0392.811286
Produced by FastQC (version 0.11.2)