FastQCFastQC Report
Wed 5 Jul 2017
000000000-ARRUV_l01n02.33200000093a2c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-ARRUV_l01n02.33200000093a2c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11901668
Sequences flagged as poor quality0
Sequence length12
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTCGTGATC3503372.9435958052266287No Hit
TCGTCGATAATC3135032.6341097735208208No Hit
ACCACATACATC3077782.5860072722579726No Hit
CGACTGTCTTAA2646002.22321778762439No Hit
GGTCACTGACAG2451242.0595768593108126No Hit
AGAGCCTACGTT2351261.9755718274110823No Hit
GTCCGAAACACT2323001.9518272564820325No Hit
GATTGGTTGCAC2226231.8705193255264725No Hit
CGTGACAATGTC2050711.7230441985106624No Hit
ATTGGGCTAGGC1780451.4959667838155122No Hit
GTCTACACACAT1774281.4907826365178394No Hit
ATGTCGAGAGAA1769071.4864050988483295No Hit
TAATCCACAGCG1753131.4730120181473723No Hit
TCTGTTGCTCTC1735111.4578712832520617No Hit
TATCAGGTGTGC1714771.4407812417553572No Hit
TACTAATCTGCG1701411.4295559244300882No Hit
GAAGAAGCGGTA1627641.3675730158159345No Hit
TGTTATCGCACA1578821.3265535553503929No Hit
TCAAGCTCAAGC1533811.2887353268466235No Hit
CCATAGGGTTCA1515631.273460157013286No Hit
CCTAGTACTGAT1467171.2327431751583056No Hit
CAGGAAGGTTAA1389561.1675338280314993No Hit
ATCGCTCGAGGA1345441.1304633938705062No Hit
CTCGAGAGTACG1318131.107517030385993No Hit
TAACGCTTGGGT1310511.1011145664624487No Hit
AGCTTGACAGCT1303221.0949893745985857No Hit
AATGGAGCATGA1234081.0368966770035932No Hit
GTGGGATGTTTC1231231.0345020546699841No Hit
TGGCAAGACTCT1201121.0092030797699953No Hit
TGACCTCCAAGA1185740.9962805213521333No Hit
ATGGACCGAACC1179410.9909619391164332No Hit
ATTAGTTCGCGT1168590.9818707764323454No Hit
CTACCGGATCAA1150610.9667636502715418No Hit
TCAACAGCATCG1130610.949959283018145No Hit
ACTATTGTCACG1121340.9421704587961957No Hit
TGTAATTGTCGC1118470.9397590320953333No Hit
GCAGGATAGATA1109760.9324407301564789No Hit
GTATTACGATCC1073830.9022516843857517No Hit
TGGCACCGATTA1038390.8724743456127325No Hit
GCATGGCTCTAA1031700.8668532847664714No Hit
GCTTCGGTAGAT1018100.8554263150341616No Hit
TCTTCCGCTACT1015820.8535106171672744No Hit
TGCAATGTTGCT1010930.849401949373819No Hit
ACGGCATGGCAT1007830.8467972724495424No Hit
TTGATGCTATGC954420.8019212096993463No Hit
TCGGAGTGTTGT952860.8006104690535815No Hit
CAACACGCACGA949590.7978629550076509No Hit
ACTCACGGTATG924060.7764121802086901No Hit
TAAACCGCGTGT911350.7657330048191565No Hit
AATCTTGCTGCA900600.7567006574204557No Hit
GCTCAGTGCAGA894110.7512476402467284No Hit
AATGTCCGTGAC864420.726301557059061No Hit
GTACCTAATTGC844500.7095644072746778No Hit
GACTCGAATCGT831140.6983390899494087No Hit
CGTCCGAAATAC821280.6900545368934842No Hit
ATGTGGGACCCA796100.6688978385214577No Hit
CCAAGTCTTACA791630.6651420624403235No Hit
ATACTTCGCAGG756910.6359696808884268No Hit
TAACACCACATC754930.6343060485303404No Hit
CGCCAAATAACC751300.6312560558738489No Hit
CTTACACCAAGT741640.6231395464904583No Hit
TGGCATACGGCA667600.5609297789183836No Hit
ATGATGACCCGT587180.49335941819247514No Hit
ACGATGCGACCA578260.4858646703974603No Hit
GACACATTTCTG560090.4705979027477493No Hit
ATGGTTGTTGGC548900.4611958592694738No Hit
CCATACATAGCT483710.4064220242070271No Hit
GAATGATGAGTG483500.40624557835086644No Hit
ATACCTTCGGTA434470.36504967202916433No Hit
TTATGCAGTCGT419130.35216072234580903No Hit
TACTTCGCTCGC402530.33821309752548967No Hit
TATGCACCAGTG385640.32402180937999614No Hit
AGTTCCCGAGTA365910.30744430108452026No Hit
ACGCGCAGATAC342310.28761514772551205No Hit
ACACCTGGTGAT340480.2860775481218263No Hit
AACACAAGGAGT339870.2855650149205977No Hit
AGCCGGCACATA308320.2590561255783643No Hit
GCCTGAATTTAC303780.2552415342118432No Hit
TAAGGTAAGGTG274730.23083319077628447No Hit
AGCAAACACCCG268320.2254473910715708No Hit
GGTCAGCTTAAC265330.222935138167188No Hit
TTACTGTGCGAT246490.20710542421448824No Hit
CGGGTTTGACGA227260.19094802510034728No Hit
ACGGGACATGCT205190.17240440583622396No Hit
GACTTGGTATTC198920.16713623670228409No Hit
TATCGTTGACCA151510.1273014841281071No Hit
ATCCCGAATTTG146280.12290714209134385No Hit
GCACACACGTTA140450.11800866903697868No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGCGTG550.0091602716.00000055
GATACTC4100.06.00000056
GATGTGT550.0091602716.00000055
TTCAGTC550.0091602716.00000054
GGACTCT550.0091602716.00000056
GGCCAAA550.0091602716.00000051
CCGTAGG550.0091602716.00000051
CAAGGAA550.0091602716.00000055
ACGTCGA1101.815024E-76.00000051
CAGTATC550.0091602716.00000055
AGTAGCG1101.815024E-76.00000053
TGATGCC953.429377E-66.00000052
AAAGCTC953.429377E-66.00000052
AGATGGC8850.06.00000054
GTCATCG650.00126188836.02
TCTGATG1451.9645086E-106.01
GAAGCTG852.4447076E-56.04
AGATCGC600.0033963786.04
ACCGGCT2400.06.03
GTCGATC1950.06.03
Produced by FastQC (version 0.11.2)