FastQCFastQC Report
Wed 5 Jul 2017
000000000-ARRUV_l01n01.33100000093a2f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-ARRUV_l01n01.33100000093a2f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11901668
Sequences flagged as poor quality0
Sequence length251
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACGTAGGGTGCGAGCGTTGTCCGGAATTACTGGGCGTAAAGGGCTCGTA162769713.676209082626064No Hit
TACGTAGGTGGCAAGCGTTATCCGGAATTATTGGGCGTAAAGCGCGCGTA153232812.874901232331467No Hit
TACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA120354910.112439701729203No Hit
TACGTAGGTGACAAGCGTTGTCCGGATTTATTGGGCGTAAAGGGAGCGCA5833134.901102937840309No Hit
TACGTAGGGTGCGAGCGTTGTCCGGAATTACTGGGCGTAAAGAGCTCGTA5831184.899464512033103No Hit
TACAGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGTA4896734.114322463036273No Hit
TACGTAGGGTGCAAGCGTTGTCCGGAATTACTGGGCGTAAAGAGCTCGTA3987853.350664797572912No Hit
TACGTAAGGACCGAGCGTTGTCCGGAATCATTGGGCGTAAAGGGTACGTA3321822.79105416148392No Hit
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA2019391.6967285593918433No Hit
TACGTAGGGGGCTAGCGTTGTCCGGAATCACTGGGCGTAAAGGGTTCGCA1919881.6131184301225676No Hit
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCA1682151.4133733187650672No Hit
TACGTAGGGTGCAAGCGTTAATCGGAATTATTGGGCGTAAAGCGAGTGCA977600.8213974713460331No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCGCGCA892290.7497184428266693No Hit
TACGTATGGAGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGGGTACGCA879000.7385519407867871No Hit
TACGTAGGGTGCGAGCGTTAATCGGAATTATTGGGCGTAAAGCGAGTGTA869590.730645485994064No Hit
TTCCAGCTCCAATAGCGTATATTAAAGTTGTTGCAGTTAAAAAGCTCGTA621030.5218008097688492No Hit
TACGGAGGGTGCGAGCGTTAATCGGAATAACTGGGCGTAAAGGGCACGCA490520.4121439112568087No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA475080.3991709397371864No Hit
TACGTAGGTGGCAAGCGTTATCCGGCATTATTGGGCGTAAAGCGCGCGTA465870.3914325286169972No Hit
TACGAAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGTA426530.3583783382295658No Hit
TACAGAGGATGCAAGCGTTATCCGGAATGATTGGGCGTAAAGCGTCTGTA398200.3345749520151293No Hit
TACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGCA384450.323021949528419No Hit
TACGGAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGTA372240.31276288332022034No Hit
TACGTAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA362370.30446992808066903No Hit
TACGTAGGTGGCAAGCGTTATCCGGAATTATTGGGCGTAAAGCGCGCGCA343420.28854779010807563No Hit
TACGTAGGGTGCGAGCGTTGTCCGGACTTACTGGGCGTAAAGGGCTCGTA332470.27934739903684086No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGGGCTCGCA325250.27328102245836466No Hit
TACGTAGGGTGCGAGCGTTGTCCGGAATTACTGGGCGTAAAGGGCTCGCA314200.26399660955086296No Hit
TACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGAGCGCA303570.2550650883556826No Hit
TACGTAGGTGGCAAGCGTTATCCGGACTTATTGGGCGTAAAGCGCGCGTA285440.23983192944047843No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGCGCGCGCA279120.23452174938840506No Hit
TACGGAAGGTCCGGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA277230.23293373668295905No Hit
TACGTAGGGCGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTTGTA272100.22862341648246276No Hit
TACGTAGGGTGCGAGCGTTAATCGGACTTACTGGGCGTAAAGCGTGCGCA271050.22774118720165948No Hit
TACGTAGGGTGCGAGCGTTGTCCGGCATTACTGGGCGTAAAGGGCTCGTA251580.21138213568047773No Hit
TACGTAGGGTGCGAGCGTTAATCGGCATTACTGGGCGTAAAGCGTGCGCA251520.21133172257871755No Hit
TACGGAAGGTTCGGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA247850.2082481211877192No Hit
TACGTAGGTGACAAGCGTTGTCCGGATTTATTGGGCGTAAAGGGCGCGCA244360.2053157591020015No Hit
GACAGAGGATGCAAGCGTTATCCGGAATGATTGGGCGTAAAGCGTCTGTA226250.19009940455405075No Hit
TACGTAGGTGGCAAGCGTTATCCGGAATTACTGGGCGTAAAGCGCGCGTA221840.18639404157467676No Hit
TACGTAGGTGGCAAGCGTTATCCGGATTTATTGGGCGTAAAGAGAGTGCA221390.18601594331147533No Hit
TACGTAGGTGTCAAGCGTTGTCCGGATTTATTGGGCGTAAAGGGAGCGCA216030.181512372887565No Hit
TACGTAGGGTGCGAGCGTTGTCCGGATTTATTGGGCGTAAAGGGCTCGTA206860.1738075705018826No Hit
TACAGAGGGTGCGAGCGTTAATCGGATTTACTGGGCGTAAAGCGTGCGTA203970.17137933943376676No Hit
TACGGGGGGTGCAAGCGTTACTCGGAATCACTGGGCGTAAAGGACGCGTA166830.140173629444209No Hit
TACGTAAGGACCGAGCGTTGTCCGGAATCATTGGGCGTAAAGGGCACGTA165110.13872845386041688No Hit
TACGTAGGGTGCGAGCGTTGTCCGGAATTACTGGGCGTAAAGCGCTCGTA148260.1245707744494301No Hit
TACGTAGGTCCCGAGCGTTATCCGGATTTATTGGGCGTAAAGCGAGCGCA147970.12432711112425586No Hit
TACGTAGGGTGCGAGCGTTGTCCGGAATTACTGGGCGTACAGGGCTCGTA145830.12252904382814242No Hit
TACGTAGGGTGCGAGCGTTGTCCGGAATTACTGGGCGTAAAGCGTGCGCA142480.11971431231319846No Hit
TACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGGGCGCA138540.11640385196427928No Hit
TACGGAAGGTCCAGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA137550.11557203578523616No Hit
TACGTAGTGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA130580.10971571379742738No Hit
TACGTAGGTGGCAAGCGTTATCCGGAATTATTGGGCGTACAGCGCGCGTA126750.1064976774684019No Hit
TACGTAGGTTGCGAGCGTTGTCCGGAATTACTGGGCGTAAAGGGCTCGTA125340.10531296957703744No Hit
TACGTAGGGTGCTAGCGTTGTCCGGAATCACTGGGCGTAAAGGGTTCGCA121970.10248143369484009No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGTG5091200.0244.369585
GTAGGGT5101000.0243.948144
GTGCGAG4655350.0243.54229
GTGACAA781100.0243.447398
TACGTAT188650.0243.311681
GTAAGGA441300.0243.029114
AGGGTGC6210600.0242.571936
TAAGGAC424200.0242.343255
GTCCCGA276050.0241.893698
TAGGTCC275100.0241.704825
GACCGAG431750.0241.6529
CGTAGGG5515950.0241.557713
GGTCCCG275850.0241.003277
TACGTAA475150.0240.926561
AGGTGAC798600.0240.674296
GTGGCAA2223500.0239.799188
CCAATAG86000.0239.587199
ACGTATG189750.0239.512512
GTAGGTC333100.0239.446854
TCCCGAG280400.0239.320629
Produced by FastQC (version 0.11.2)