FastQCFastQC Report
Wed 3 Aug 2016
000000000-ARM7R_l01n02.332000000663e6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-ARM7R_l01n02.332000000663e6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15168580
Sequences flagged as poor quality0
Sequence length12
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATGTTCTGCT300986319.842747310559066No Hit
AGTAGAGGGATG9673266.377169121961318No Hit
ATCGATCTGTGG2721861.7944065957393507No Hit
GAAGTTGGAAGT2597881.7126718519465896No Hit
CAAGCATGCCTA2485431.6385383470305066No Hit
CACGGTTGTGAG2320491.5298004163870316No Hit
CGCAGCGGTATA2306601.5206433298304785No Hit
GACGGAACCCAT2228261.4689970979485225No Hit
GTCATATCGTAC2093211.379964373725161No Hit
TCCAAAGTGTTC2065261.3615381268385043No Hit
TACACGATCTAC2004081.3212047535102165No Hit
CCTGAACTAGTT1981751.3064835337256355No Hit
TCACGGGAGTTG1799381.1862547450057948No Hit
GGAAACCACCAC1756941.1582758570677019No Hit
GAACTAGTCACC1718711.1330724431687078No Hit
AGGCTACACGAC1694661.1172173004987942No Hit
ACACCTGGTGAT1681341.1084359907123804No Hit
ACGTGTACCCAA1598301.0536912486205037No Hit
GGCCACGTAGTA1591731.0493599269015295No Hit
GTCAATTGACCG1567701.0335179693814451No Hit
GTTGGTCAATCT1547821.0204119304509718No Hit
GAACCAAAGGAT1546491.019535117987313No Hit
TTGCGCATACTA1476870.9736376114309975No Hit
ACGGGACATGCT1470190.9692337713879612No Hit
TTACTGTGCGAT1431320.9436084326944248No Hit
GAGTGGTAGAGA1403480.9252547041318304No Hit
CCAGTGTATGCA1398610.9220441201483593No Hit
GCGATATATCGC1375330.9066966057468795No Hit
GTATGCGCTGTA1318910.8695012980780008No Hit
CTTCGGCAGAAT1313960.8662379734952118No Hit
GGATCGCAGATC1312510.8652820501325768No Hit
GTCTAATTCCGA1303120.8590916222876499No Hit
GCACGACAACAC1276900.8418058908612408No Hit
TATCGTTGACCA1275650.8409818189969002No Hit
ACACGTAAGCCT1266570.8349957609743299No Hit
GACTTTCCCTCG1216340.8018812571776659No Hit
CTTGTGTCGATA1191320.7853866347410239No Hit
GCACACACGTTA1174920.7745748118808747No Hit
ATTCCTGTGAGT1167100.7694194182975598No Hit
TCCTCTGTCGAC1149340.7577110052490081No Hit
GACAGGAGATAG1120650.738796907818662No Hit
CGATCCGTATTA1119860.7382760944003988No Hit
CAGTGCATATGC1054430.6951408767333528No Hit
GAATCTTCGAGC1052550.6939014726493844No Hit
CTAGCGAACATC996400.6568841645032033No Hit
GAGGCTCATCAT959060.6322674897716201No Hit
ACATTCAGCGCA950430.6265780976202123No Hit
TAGGATTGCTCG947050.6243498072990352No Hit
ATGTGCACGACT940230.6198536712071928No Hit
GACTTGGTATTC922120.6079145180366257No Hit
TAGCTCGTAACT907410.5982168403370651No Hit
ACGCGCAGATAC870380.5738045354278384No Hit
CAGCGGTGACAT850890.5609556069190392No Hit
GCTGATGAGCTG850560.5607380519468532No Hit
AATCCGTACAGC821940.5418701025409103No Hit
AATCAGTCTCGT741070.4885559492055288No Hit
CTAACCTCCGCT738940.48715173074869234No Hit
ATCCCGAATTTG738590.48692099062667704No Hit
TGAGTCACTGGT717600.47308317588066917No Hit
AAGGAGCGCCTT712000.4693913339284231No Hit
TTCCTAGGTGAG680940.4489147962432871No Hit
CACGCCATAATG664930.4383600838048123No Hit
CAGGCGTATTGG655650.43224217428394746No Hit
CTATTTGCGACA628810.41454770321282547No Hit
GGAGACAAGGGA601760.39671478806849425No Hit
CCTCGTTCGACT542080.3573703009774152No Hit
ATCGGCGTTACA457800.3018080795961125No Hit
ATGAGACTCCAC399710.2635118119164747No Hit
CGGAGCTATGGT394590.2601364135601355No Hit
AGCTGTTCTGCT189780.12511388673165186No Hit
TAGGAACTGGCC151830.10009506493027034No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACAGTA806.539317E-56.00000051
GACACGG806.539317E-56.00000054
CGGTTGC806.539317E-56.00000053
TGGGTCG806.539317E-56.00000053
GTGCTTA1054.830745E-76.00000053
CATTTCG1054.830745E-76.00000053
TACGTGG806.539317E-56.00000052
TCTGCAG751.7513495E-46.03
GCCCCGT650.00126196276.02
TGCCCGA600.0033965476.04
GCGACTC600.0033965476.03
GTCGAGG5300.06.02
AAGAGTT953.4297955E-66.05
ACGTTTG1303.6470738E-96.02
TGGACGT953.4297955E-66.06
GCAACGG550.0091606676.02
GTGGGCT1001.286593E-66.05
GTGGGAC650.00126196276.05
AGTGGGC751.7513495E-46.04
GCCATGC650.00126196276.05
Produced by FastQC (version 0.11.2)