FastQCFastQC Report
Thu 22 Dec 2016
000000000-ARK2F_l01n03.333000000746f3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-ARK2F_l01n03.333000000746f3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14789562
Sequences flagged as poor quality0
Sequence length251
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTGTTGAAAGTTGTAACTATTAAAGTTTTTCAGACGCTGATTGCAACT264120317.85856132859107No Hit
CATTGTTGAAAGTTGTAACGATTGTTATTGTCGGAACAGGTAATGCTAGA5916054.000152269553351No Hit
CGTTGTTAAAAGTTTTAATTTATTAATTAAGTTTACTCAGACTGCAAAGT3750602.535977738894499No Hit
CATTGTTGAAAGTTGTAACGATTGTTGTTGTCGGAACAGGTAATGCTAGA3462072.340887444807358No Hit
CGTCACTGAAAGTTTTAATTGATTTTTATATATCACACATTCATGTTAAC3274322.213939804302521No Hit
CGTTGTTGAAAGTTTTAATTTATTAATTAAATTTACTCAGACTGCAAAGT2949551.9943457419496264No Hit
CGTTGTTGAAAGTTTTGACTATTAAATAATCGGTTGACATTAAATAAAAT2277141.539694008517629No Hit
CGTTGTTGAAAGTTTTTAATATTTTAAAATTTCCAGTTACGAAAATTCTT2254061.524088407756768No Hit
CGTTGCTGAAAGTTGTATAGTGTTTTATAGGCACAGAGGCCTGTGATAAT1737421.1747609564096624No Hit
CGTTGTTGAAAGTTTTAATTATTGTTTAACTAAAAACTCAGACTGCAAAC1698661.1485532837280779No Hit
CGTTGTCGAAAGTTGTATTAACTTTTTAGGGTCTGTCAAGACCATGATTA1661831.123650585460205No Hit
CGTTGTTGAAAGTTTTAACGTATTCTGTTTCGTAGTTCAGAGCTCAGGTG1575711.0654203282017412No Hit
CGTTGTTGAAAGTTGTAAATAGTTTGTTTTGTTTTGTTCAGACGTGTACT1565151.0582801573163558No Hit
CGTTGCTGAAAGTTTTATAAAACTTTTAATATTTCAACAGCTTAACTGCT1304700.8821762267198987No Hit
CGTTGTTAAAAGTTTTATTAATTATAATAAGACTACATTTGTTACAAATT1297560.8773484975417122No Hit
CGTTGCTGAAAGTTTTATTTTTATTTATGCTCAATTAAGAGACTGTTACA1197810.8099022810817522No Hit
CGTTGTTGAAAGTTTTATTATGTTATAATAAGACTACATTTGTTACTAAA1077430.7285070375985442No Hit
CGTTGTTGAAAGTTTTGTTTTTATTTAACAATAATACTTAGATTCAAGAC944780.6388154023763516No Hit
CGTTGCTGAAAGTTGTATTATGTTTTTTAAAAGAGTTGCTACGCTCTTCA854610.5778467273067316No Hit
CGTTGTTGAAAGTTTTATTAAATTAACTTTTTACTCCGACGTAAGAATGC827120.5592592938181672No Hit
CGTTGTTGAAAGTTTTGTTTTGTTTTGTTTTGTATATTGATCATTCATTA790420.5344444953812696No Hit
CGTTGTTGAAAGTTTTATTTTGTTATAATAAAACGACGTTCATTACACAT788240.532970482831067No Hit
CATTGTTGAAAGTCGTAACGATTGTTATTGTCGGAACAGGTAATGCTAGA771530.5216719737879999No Hit
CGTTGTTGAAAGTTGTAACTATTATAGTTTTTCAGACGCTGATTGCAACT734930.4969247906056988No Hit
CGTTGTTGAAAGTTGTAATAATTTTGTTTTTACTGACGCTGACTGCAATT662280.447802308141377No Hit
CGTTGTTGAAAGTTGTATTATGGTTTATAGGCACAAGGCCCATTAAATGA550260.3720596999424324No Hit
CGTTGTTGAAAGTTTTGACTTATTAAATAAGACACTCAGATAATCAACGA500400.3383467340006418No Hit
CGTTGTTGAAAGTTTTAACTAATTTAGCTAGTTTCTCAGACTGCAAACTT452070.30566828145417696No Hit
CGTTGTTGAAAGTTTTAACGATTTTTTATATCGGTACTCAGACTGCAACC439920.2974530280207081No Hit
CGTTGTTGAAAGTTTTAACGATTTGATTTGATACTCAGACGGCAACCTTC423220.28616128050310075No Hit
CGTTGTTGAAAGTTTTATTATGTTATAATAAGACTACATTTGTTACAATA388340.2625770796998586No Hit
CGTTGTTGAAAGTTTTGATTTATTTGTTTGTTTTACTCAGAAGTTCCACT386590.2613938127444207No Hit
CGTTGTTGAAAGTTTTAATTTATTTGCTTGTTTTACTCAGAAGAAACATT385300.2605215759601265No Hit
CGTTGTTGAAAGTTGTAACTATTAAAGCTTTTCAGACGCTGATTGCAACT384270.259825138837783No Hit
CGTTGTTGAAAGTTTTAAATAATTTATATTTTCACTCAGACTTCAATCTT368760.24933801284987345No Hit
CGTTGTTGAAAGTTTTGACTATTAGTTAATCAAGTTGACAATTAAATAAA346720.23443561073681563No Hit
CGTTGTTGAAAGTTTTGAAGATTAAAATTCAAATTGACTAGTTTCTAGAG338300.22874240629979442No Hit
CGTTGTTGAAAGTTTTGAAGATTTTAATTTTTGTTAAAAATTTTCATGAC315600.21339374350639997No Hit
CGTTGTTGAAAGTTTTAATTTATTAGTTAAATTTACTCAGACTGCAAAGT289010.1954148473092036No Hit
CGTTGTTGAAAGTTGTAACTATTAATGTTTTTCAGACGCTGATTGCAACT283070.1913985011861744No Hit
CGTTGTTGAAAGTTTTAACGATTTTTTATATCGGTACTCGGACTGCAACC260660.1762459226311097No Hit
CGTTGTTGAAAGTTGTAACTATTATTGTTTTTCAGACGCTGATTGCAACT231160.1562994225251566No Hit
CGTTGTTGAAAGTTTTATTTTGTTATAATAAAACGACGTTCACTACACAT211560.14304683262425216No Hit
CGTTGTTGAAAGTTGTAGCTATTAAAGTTTTTCAGACGCTGATTGCAACT211390.1429318866914382No Hit
CGTTGTTGAAAGTTTTGTTATGTTATAATAAGACTACATTTGTTACAATA207890.1405653527805624No Hit
CGTTGTTGGAAGTTGTAACTATTAAAGTTTTTCAGACGCTGATTGCAACT196910.13314119782587205No Hit
CGTTGTTGAAAGTTTTATTATGTTATAATAAGACTACATTTGTTACATTA193080.130551533574828No Hit
CGTTGTTGAAAGTTTTGATTGATTATATGTATCACTCAGACGCAAGAGAT180460.12201848844475584No Hit
CGTTGTTGAAAGTTGTAACTATTAAAGTTTTTCAGGCGCTGATTGCAACT174490.1179818577453477No Hit
CGTTGTTGAAAGTTATATTAGTTTATAACGATTTTACATTCAATGACTTG167180.11303918263434712No Hit
CGTTGTTAAAAGTTTTAATTTATTAATTAAGTTTACTCGGACTGCAAAGT166730.11273491398866309No Hit
CGTTGTTGAAAGTTTTGATTCATTTTGTTTTCGGGCTTTCGCCCCTCAGA163320.1104292338069241No Hit
CGTTGTTGAAGGTTGTAACTATTAAAGTTTTTCAGACGCTGATTGCAACT161480.1091851131223494No Hit
CGTTGTTGAAAGTTTTGTTTTAGATTTTTGAACTTAACGGTGGTTGCTTT149460.10105775951985596No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAAAGT11286350.0244.14027
GAAAGTT11479050.0243.499398
TGTTGAA10162000.0243.085564
TTGAAAG10115750.0242.860026
GTTGAAA10098600.0241.73195
TTGTTGA10322500.0241.307543
CGTTGTT9592350.0239.857271
CTGAAAG1285650.0239.845056
GCTGAAA820550.0238.102635
TGCTGAA847850.0232.559834
AAAGTTG7155700.0231.9439
GTTGTTG9452850.0226.493772
TGTCGAA331800.0222.774114
TCGAAAG331750.0222.032246
TTGCTGA906900.0220.67353
CGTTGCT916150.0217.544741
GTCGAAA338300.0216.864325
CACTGAA496100.0215.665044
GTTGCTG944350.0213.439972
TTGTCGA363800.0205.940633
Produced by FastQC (version 0.11.2)