FastQCFastQC Report
Thu 22 Dec 2016
000000000-ARK2F_l01n02.332000000746f6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-ARK2F_l01n02.332000000746f6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14789562
Sequences flagged as poor quality0
Sequence length12
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCACCTACTCCA644484943.577010597068394No Hit
CCTCGTTCGACT5149683.481969242902528No Hit
AGCTGGAAGTCC4094392.7684322226716382No Hit
CAAGCATGCCTA3837372.5946474953078393No Hit
CCTGAACTAGTT3361502.272886783259707No Hit
AAGAGATGTCGA2998272.0272878939890173No Hit
TCGAGGACTGCA2793961.889143167323008No Hit
ATGTGCACGACT2487861.682172873003271No Hit
ATGAGACTCCAC2301711.5563070765719769No Hit
TACAGATGGCTC2253451.5236759547037297No Hit
TTGCGCATACTA2228761.506981748343866No Hit
ACATTCAGCGCA2218721.500193176782382No Hit
TGAGTCACTGGT2038811.3785465722379067No Hit
ACACCTGGTGAT2000501.3526431682020061No Hit
ATCGATCTGTGG1749621.1830100174704294No Hit
TAGGAACTGGCC1675501.13289359076354No Hit
AGATGTTCTGCT1667931.1277751159905884No Hit
GAACTAGTCACC1496271.0117067699503204No Hit
CCAGTGTATGCA1415190.9568843215235178No Hit
ACGCGCAGATAC1308370.8846577065635886No Hit
CAGCTAGAACGC858440.5804363915577757No Hit
GGCCACGTAGTA704650.4764508915138934No Hit
ACACGTAAGCCT574970.38876742935321545No Hit
AGGCATCTTACG463490.31338994352909166No Hit
TCACCTACTCCA434460.2937612351197419No Hit
CCACCTACTCCT415140.2806979679317075No Hit
CCCCCTCCTCCC368670.24927715912073664No Hit
GAATCTTCGAGC345050.23330643598505488No Hit
GAATAGAGCCAA338210.22868155257065761No Hit
CCACCTACTCCC314750.21281901384233012No Hit
CCACCTACTCAA313910.21225104570371994No Hit
CTGCTAACGCAA302160.20430625328863697No Hit
GACGGAACCCAT290070.19613156900792603No Hit
GCGATATATCGC279310.18885616761334784No Hit
CCCCCTACTCCA276840.1871860708248155No Hit
AATCCGTACAGC231570.15657664506900204No Hit
CCACCTATCCAA207430.14025432260941872No Hit
CCCCCTACTCCC203170.1373739127636099No Hit
CCACCTACCCAA198340.1341080959666013No Hit
CCACTACTCCAA173920.11759645079414793No Hit
TCGAGTACTGCA173690.11744093570857608No Hit
CCTCGTTCGCCT161570.1092459668514862No Hit
GTCAATTGACCG159710.10798832311599221No Hit
ACACCTACTCCA150390.10168658138760295No Hit
AGCTGTAAGTCC148290.10026666104107748No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTGGA1552.7284841E-116.00094751
ATGGGCA600.00339055436.0009471
CCTCGGT852.4390152E-56.0009471
TCTAGGA704.6878183E-46.0009471
GGCCCCG953.420564E-66.0009471
GGCCACT600.00339055436.0009471
AGACCTA704.6878183E-46.0009471
CCCCCGA1351.369699E-96.0009471
AGCTGCA852.4390152E-56.0009471
TCCCGTA550.00914571256.0009471
CCGCCGA600.00339055436.0009471
CGCCACG1351.369699E-96.0009471
ACGCGCT1101.8097126E-76.0009471
GCCCCTA650.00125956886.00094651
AACCCTC650.00125956886.00094651
CTGCGAC1054.8164657E-76.00094651
GGCCACG73000.06.00094651
TCCAGGA751.7475642E-46.00094651
TCCCGAT1156.805021E-86.00094651
ACCCCTG1451.9463187E-106.00094651
Produced by FastQC (version 0.11.2)