FastQCFastQC Report
Thu 22 Dec 2016
000000000-ARK2F_l01n01.331000000746f9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-ARK2F_l01n01.331000000746f9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14789562
Sequences flagged as poor quality0
Sequence length251
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGGTGAACCTGCGGAAGGATCATTACCTAGAGTTGTAGGCTTTGCCTG308108620.832841432356144No Hit
GTAGGTGAACCTGCGGAAGGATCATTAACCGTTACAAACAGTGTGTGCTG12793048.650046566625841No Hit
GTAGGTGAACCTGCGGAAGGATCATTACCGAGTGCGGGCCCTCTGGGTCC4213832.8491918827616396No Hit
GTAGGTGAACCTGCGGAAGGATCATTATCGAAAAAGATAAAGAAAAACAT3712382.510135188587735No Hit
GTAGGTGAACCTGCGGAAGGATCATTAAAGAAATTTAATAATTTTGAAAA3286802.222378188076158No Hit
GTAGGTGAACCTGCGGAAGGATCATTACAGAATGAAAAGTGCTTAACTGC2861851.9350471636685387No Hit
GTAGGTGAACCTGCGGAGGGATCATTACAAGTGACCCCGGTCTAACCACC2308771.5610807135464864No Hit
GTAGGTGAACCTGCGGAAGGATCATTAACGAATCAAACAAGTTCATCTTG2248541.5203560457030438No Hit
GTAGGTGAACCTGCGGAAGGATCATTATTGAATAAACTTGATGGGTTGTT2114751.4298935965784516No Hit
GTAGGTGAACCTGCGGAGGGATCATTACAAGTTGACCCCGGCCCTCGGGC1992731.347389462919862No Hit
GTAGGTGAACCTGCGGAAGGATCATTATCGTAGGGGCCTCGCCCCCTTCG1958501.324244761271497No Hit
GTAGGTGAACCTGCGGAAGGATCATTAGTGATTAATTATTGAGTGATAAA1918461.2971716133310778No Hit
GTTGGTGAACCAGCGGAGGGATCATTACCGAGTTCTCGCAACTCCCAAAA1488411.0063922109390393No Hit
GTAGGTGAACCTGCGGAAGGATCATTAATGATTAACTGTCTGTCGAGCTT1477890.9992790861554925No Hit
GTAGGTGAACCTGCGGAAGGATCATTATAGAATTTGATGAGATGCTGGCT1429060.9662625573360456No Hit
GTAGGTGAACCTGCGGAGGGATCATTACAAGTGACCCCGGTCTTACCACC1402820.9485203145299368No Hit
GTAGGTGAACCTGCGGAAGGATCATTAATGCATTGAAACTGCCCTCTTTT1396030.9439292387428376No Hit
GTAGGTGAACCTGCGGAAGGATCATTAGTGATTGCCTTTATAGGCTTATA1288500.8712225554752737No Hit
GTAGGTGAACCTGCGGAAGGATCATTACCGAGTGTTTGGATGCCCCTTCT1199940.8113424860046564No Hit
GTAGGTGAACCTGCGGTAGGATCATTACCTAGAGTTGTAGGCTTTGCCTG1175390.7947429410012278No Hit
GTAGGTGAACCTGCGGAGGGATCATTACAAGTGACCCCGGTTTACCACCG1032980.6984520569304216No Hit
GTAGGTGAACCTGCGGAAGGATCATTATTGAAAAACTTTGTGGGCTGTAG1027440.6947061718257782No Hit
GTAGGTGAACCTGCGGAGGGATCATTAACGAGTTAGGGTCTCACCGGCCC945000.6389641559364638No Hit
GTAGGTGAACCTGCGGAAGGATCATTACCGAGTGCGGGTCCCTCGTGGCC907290.6134664434281422No Hit
GTAGGTGAACCTGCGGAAGGATCATTAGTGATTTGGCCCTCTGTGGCCCT880150.5951156633306652No Hit
GTAGGTGAACCTGCGGAAGGATCATTACTTATATGACGGCAGACTGGGGT776670.5251473978742576No Hit
GTAGGTGAACCTGCGGAAGGATCATTACCGAGTGAGGGCCCTTTGGGTCC735400.4972425823023021No Hit
GTAGGTGAACCTGCGGAAGGATCATTAATGAATTCACTATGGAGTTGTTG722890.4887839139522861No Hit
GTAGGTGAACCTGCGGAAGGATCATTAATGAATTTAGATTGAACCATAGG643890.43536786282108964No Hit
GTAGGTGAACCTGCGGAAGGATTATTACTGAGTGAGGGCCCCTCGGGGTC586130.3963132917661794No Hit
GTAGGTGAACCTGCGGAGGGATCATTACTGAGTGAGGGAGAAATCCCGAC509270.344344207083347No Hit
GTAGGTGAACCTGCGGTAGGATCATTAACCGTTACAAACAGTGTGTGCTG490710.33179481583024567No Hit
GTTGGTGAACCAGCGGAGGGATCATTACAGAGTTATCCAACTCCCAAACC465860.3149924250630276No Hit
GTAGGTGAACCTGCGGAAGGATCATTAGAGAACCTGGGCCTGCGCTACTT457820.3095561585934729No Hit
GTAGGTGAACCTGCGGAAGGATCATTACAGAAATAGGAGAAGGTTGCTTA429750.29057655662824905No Hit
GTAGGTGAACCTGCGGAAGGATCATTATAGTATTCTATTGCCAGCGCTTA415140.2806979679317075No Hit
GTTGGTGAACCAGCGGAGGGATCATTACCGAGTTTACAACTCCCAAACCC394540.26676922548483856No Hit
GTAGGTGAACCTGCGGAGGGATCATTACTGAGTGAGGGAGCAATCCCGAC374530.2532394130400887No Hit
GTTGGTGAACCAGCGGAGGGATCATTACCGAGTTATTCAACTCATCAACC370450.2504807106525535No Hit
GTAGGTGAACCTGCGGAAGGATCATTACCTAGTGTTGTAGGCTTTGCCTG359980.24340139349630502No Hit
GTAGGTGAACCTGCGGAAGGATCATTACTGAGTGAGGGCCCCTCGGGGTC310070.20965461992721623No Hit
GTAGGTGAACCTGCGGAAGGATCGTTACCTAGAGTTGTAGGCTTTGCCTG230400.15578554659022356No Hit
GTAGGTGAACCTGCGGAAGGATCATTACCTAGAGTTGTAGGCCTTGCCTG216770.14656958738872725No Hit
GTAGGTGAACCTGCGGAGGGATCATTACCGAGTGTGGCGCTCCGGCGCCT214530.14505500568576676No Hit
GTAGGTGAACCTGCGGAAGGATCACTAGAGATTTCGCCCTCCGGGGCTCT203540.13762408920561678No Hit
GTAGGTGAACCTGCGGAAGGATCATTAGTGAATATAGGACGTCCAACTTA185440.1253857281236591No Hit
GTAGGTGAACCTGCGGAAGGATCATTACCTAGAGTCGTAGGCTTTGCCTG179790.12156546623895961No Hit
GTAGGTGAACCTGCGGAAGGATCATTAAAATACTTTTCAAAACTTTGTTT173230.11712990553743241No Hit
GTTGGTGAACCAGCGGAGGGATCATTACCAGAGTGCCCTAGGCTCTCCAA160310.1083940146435709No Hit
GTAGGTGAGCCTGCGGAAGGATCATTACCTAGAGTTGTAGGCTTTGCCTG154740.10462784496254858No Hit
GTAGGTGAACCTGCGGAAGGATCATTAATGAAATTTATGACTGGAGTTGT151960.10274814088476725No Hit
GTAGGTGAACCTGCGGAAGGATCATTACCTAGAGTTGTAGGCTTCGCCTG148460.10038160697389145No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGTGAA12472000.0244.784883
TAGGTGA12510150.0244.699392
GTGAACC13027950.0244.509175
GGTGAAC13011000.0244.354134
TGAACCT12643400.0244.278156
GTAGGTG12613900.0244.260941
ACCTGCG12729900.0244.091589
GAACCTG12706850.0243.60797
AACCTGC12724150.0243.171758
GTGGAGT324350.0235.9357245
ACCAGCG441300.0230.9549
GTTGGTG465950.0230.408841
TTGGTGA458900.0228.342792
TGGTGAA472350.0221.114643
GAACCAG453050.0219.934897
GTCGGTG79400.0219.080581
AGGTGGA58200.0216.16413
AGTGACG434350.0210.39482245
GGCGAAC47150.0204.469764
AACCAGC495200.0201.140448
Produced by FastQC (version 0.11.2)