FastQCFastQC Report
Tue 20 Dec 2016
000000000-ARK0Y_l01n03.333000000746d9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-ARK0Y_l01n03.333000000746d9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15814339
Sequences flagged as poor quality0
Sequence length251
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTGTTGAAAGTTTTATTAAATTAACTTTTTACTCCGACGTAAGAATGC177954211.252711858522826No Hit
CGTTGTTGAAAGTTTTAATTTATTAATTAAATTTACTCAGACTGCAAAGT6194093.916755546975438No Hit
CGTTGTTGAAAGTTTTGATTGATTTAATAATCGCTCAGACGCAATGATGC5995043.7908887624073317No Hit
CGTTGTTAAAAGTTTTAATTTATTAATTAAGTTTACTCAGACTGCAAAGT5259963.326070093729495No Hit
CGTTGTTGAAAGTTTTATTATGTTATAATAAGACTACATTTGTTACTAAA4507722.8504005131039625No Hit
CGTTGTTGAAAGTTTTAACGATTTGATTTGATACTCAGACGGCAACCTTC1915481.211229884473831No Hit
CGTTGTTGAAAGTTTTATTTTGTTATAAAATTTAATACATTCATAGACTT1628321.0296478404819829No Hit
CATTGTTAAAAGTTGTCACTCATTGGTTATAATGATATTTCATGTCGCCG1577950.9977969992928569No Hit
CGTTGTTGAAAGTTTTGATTTATTCAAAATTTTAACTCAGACGACCGGTT1549570.9798512603024383No Hit
CGTTGTTGAAAGTTTTGTTTTGTTATAATTTTGTATACATTCATAGACTT1309070.827774085277924No Hit
CATTGCCAAAAGTTGTATATAGTTTGGTTTAGCACAGCAAGCTGTACCAA1182290.7476063337203028No Hit
CGTTGTTGAAAGTTTTATTATTGTTATAATAAGATTACATTCATTACATT1085230.6862316534380603No Hit
CGTTGTTGAAAGTTTTATTATGTTATAATAAGACTACATTTGTTACAATA1071890.6777962708400269No Hit
CGTTGTTGAAAGTTTTGTTTTTATTTAACAATAATACTTAGATTCAAGAC1067160.6748053143416237No Hit
CGTTGCTGAAAGTTGTATTATATGCTTTTACACATGATAATGACATTCTA1047010.6620637131909213No Hit
CGTTGTTGAAAGTTTTATTATGTTATAATAAGACTACATTTGTTACATTA1012050.6399571932788338No Hit
CGTTGTTAAAAGTTTTAATTTATTAATTAAATTTACTCAGACTGCAAAGT925760.5853927881525747No Hit
CGTTGCTGAAAGTTTTATTATATATTTATGCTCAATTAAGAGACTGGTAC833500.5270533279955615No Hit
CGTTGTTGAAAGTTTTTAATATTTTAAAATTTCCAGTTACGAAAATTCTT744110.470528676538425No Hit
CGTTGTTGAAAGTTGTAACTATTAAAGTTTTTCAGACGCTGATTGCAACT713900.45142575987526257No Hit
CGTTGTTGAAAGTTATTTTGTTTAATTTTATCAGACATTCTAGACATAAG689660.43609789824285417No Hit
CGTTGTTGAAAGTTTTGACTATTAATAAATATAATCAGTTGACTAGTTTA657700.4158883909090351No Hit
CGTTGTTAAAAGTTGTAATTATTAATTTTTTTTACTGACGCTGATTGCAA653000.41291640453641465No Hit
CGTTGTTGAAAGTTTTGAATATTTGTATAATTCATGACTGACATCAAAAA615590.3892606576854082No Hit
CGTTGTTGAAAGTTTTAAATAATTTATATTTTCACTCAGACTACAATCTT610000.3857258909145681No Hit
CGTTGTTGAAAGTTTTAACGATTTTTACTATCGGTACTCAGACTGCAACC579590.3664965067461877No Hit
CGTTGTTGAAAGTTTTAATTGATTGTGTTTTTACTCAGAAGATACTGAAG517150.3270133516171621No Hit
CGTTGTTGAAAGTTTTGATTTATTTGTTTTTTATACTCAGAAGTTCCACT463070.2928165382062443No Hit
CGTTGTTGAAAGTTGTATTAACTTGTTATGATTCAAACGTTCCAATAACA457250.28913633380440373No Hit
CGTTGTTGAAAGTTTTATTATATTAACTTTTTACTCCGACGTAAGAATGC453430.286720804454742No Hit
CGTTGTTGAAAGTTTTGAAGATTATTTTTGAATTTAATCAAACAAATTGA443660.280542866824848No Hit
CGTTGTTGAAAGTTTTGATTTATTTATGGTTTTACTCAGAAGTTACATAT394350.24936230341337695No Hit
CGTTGTCGAAAGTTGTGTATAGTTTGTTGGCCAGTGACGGTCCAACCAAG361610.22865957280920815No Hit
CGTTGTTGAAAGTTGTATTTGTGCGAGTTAACGCAAGGTACATTCTGATA327180.2068881917859482No Hit
CGTTGTTGAAAGTTTTGATAAATTTTGTATTTCTGGTGACTCTCTGGTTC312020.19730195489043204No Hit
CGTTGTTGAAAGTCTTGTTTTTATTTAACAATAATACTTAGATTCAAGAC302340.1911809276378861No Hit
CGTTGTTGAAAGTTTTGATATTTTTATTTGTTGACTAAGTAAATTATTAG302060.19100387313058106No Hit
CGTTGTTGAAAGTTTTGACTTATTAAATAAGACGCTCAGATAATCAACTA289970.18335891244015953No Hit
AGTTGTTGAAAGTTTTATTAAATTAACTTTTTACTCCGACGTAAGAATGC261670.16546376045182792No Hit
CGTTGTTGAAAGTTTTATTAAATTATCTTTTTACTCCGACGTAAGAATGC251300.15890642030628027No Hit
CGTTGTTGAAAGTTTTGAAGATTAAAATTCAAATTGACTAGTTTCTAGAG247430.15645927408031407No Hit
CATTGTTGAAAGTTTTGACTGATTTTATATTCAGACTCAGACTGCATCAC229300.14499499473231223No Hit
CGTTGTTGAAAGTTTTATTTAATTAACTTTTTACTCCGACGTAAGAATGC205670.1300528589908184No Hit
CGTTGTCGAAAGTTTTATTAAATTAACTTTTTACTCCGACGTAAGAATGC200260.12663191297467444No Hit
CGTTGTTGAAAGTTTTGTTTGATTTAATAATCGCTCAGACGCAATGATGC195740.1237737473567501No Hit
CGTTGTTGAAAGTTTTAAATAATTTATATTTTCACTCAGACTTCAATCTT192970.12202217240948231No Hit
CGTTGTTGAAAGTTTTAACGATTTTTCGTATATGAAAACTCAGACGGCAA190650.12055514934895477No Hit
CGTTGTTGAAAGTTTTATTTTATTAACTTTTTACTCCGACGTAAGAATGC179920.11377016769401489No Hit
CGTTGTTGAAAGTTTTAATTTATTAATTAAGTTTACTCAGACTGCAAAGT174320.11022907754791396No Hit
CATTGTTGAAAGTTTTAAATTTTGGGGGGTTTCTGAATTTTTTACTGAGA172980.10938174526295408No Hit
CGTTGCTGAAAGTTTTGTTTTGTTTATTGCTCTTTCGAGACTTGTTTTAC166980.10558772010641736No Hit
CGTTGTCGAAAGTTGTGTTATATTTGTTGGCCAGTGACGGTCCAACGATA162550.10278646486584105No Hit
CGTTGTTGAAAGTTTTATTTTGTTATATAATTTAATACATTCATAGACTT158950.10051004977191902No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAAAGT10601800.0244.343547
TTGAAAG10270250.0244.306466
GTTGAAA10278400.0243.842185
GAAAGTT10730350.0243.664158
TGTTGAA10635650.0242.676324
AAAGTTT10958200.0240.094579
GCTGAAA394150.0236.702385
TTGTTGA11230100.0235.62723
GTTAAAA1385650.0233.905625
CGTTGTT12575350.0232.395311
GTTGTTG11738400.0231.655262
CTGAAAG405050.0230.78636
TGCTGAA440300.0218.875764
TGTTAAA1525050.0217.706044
AAAAGTT1712600.0214.864848
AAAGTTG1541900.0214.078929
TAAAAGT1526550.0213.984687
CATTGCC186600.0213.451321
TTAAAAG1524650.0213.078956
TCGAAAG168100.0209.438376
Produced by FastQC (version 0.11.2)