FastQCFastQC Report
Tue 20 Dec 2016
000000000-ARK0Y_l01n02.332000000746dc.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-ARK0Y_l01n02.332000000746dc.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15814339
Sequences flagged as poor quality0
Sequence length12
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAACCTCCGCT195433412.357987267125107No Hit
AGGCTACACGAC12837008.117316822410345No Hit
CCTCGTTCGACT4185182.646446367439069No Hit
GACAGGAGATAG3599292.2759661342785176No Hit
CTAGCGAACATC3413922.1587497270673155No Hit
AATCCGTACAGC3285132.0773109770822544No Hit
GCTGATGAGCTG3283912.0765395253004253No Hit
ATTCCTGTGAGT3272962.0696154293897457No Hit
TCACGGGAGTTG3012981.9052203193570088No Hit
CGATCCGTATTA2938961.8584146956758671No Hit
CGGAGCTATGGT2869091.8142332727279968No Hit
TCCCAGAACAAC2627941.6617450783115248No Hit
TCCGACACAATT2521491.594432748659302No Hit
GAAGTTGGAAGT2488431.5735276700467846No Hit
GACTTGGTATTC2471461.5627969022290467No Hit
GAATCTTCGAGC2358731.4915134929129823No Hit
TATCGTTGACCA2241141.4171569232201233No Hit
ACGCGCAGATAC2240691.4168723713333828No Hit
ACGGGACATGCT2143491.3554091637974879No Hit
CCTGAACTAGTT2078931.3145854531131527No Hit
TTAGGGCTCGTA2075521.3124291821491876No Hit
CACGGTTGTGAG2064261.3053090616054202No Hit
GAGTGGTAGAGA2036811.2879513965142646No Hit
GAGGCTCATCAT1897951.2001450076414828No Hit
AGCTGGAAGTCC1713401.0834471172016737No Hit
TCGAGGACTGCA1704501.0778193132194775No Hit
GACGGAACCCAT1618891.0236848976109592No Hit
GGTGTCTATTGT1597881.0103994861878198No Hit
AATCAGTCTCGT1560470.9867437393368133No Hit
CAGCGGTGACAT1541310.9746281523369392No Hit
GGAAACCACCAC1504530.9513707781273691No Hit
TAGGAACTGGCC1369410.8659293316021619No Hit
GAACCAAAGGAT1346950.8517270307661926No Hit
AGGCATCTTACG1269620.8028283698736951No Hit
CAGGCGTATTGG1193590.7547517477651137No Hit
GCACGACAACAC1126890.712574834774947No Hit
AAGGAGCGCCTT1063080.6722253772351787No Hit
ATGAGACTCCAC970100.6134306340593811No Hit
CAGCTAGAACGC955050.6039139542917349No Hit
TACAGATGGCTC826910.522886223698632No Hit
TACACGATCTAC820160.5186179453975281No Hit
ACACGTAAGCCT796230.5034861083982075No Hit
AGTAGAGGGATG757570.4790399396395891No Hit
CAAGCATGCCTA733170.46361090400300636No Hit
ATGTGCACGACT612670.387414232109227No Hit
ACACCTGGTGAT538020.34021023578664905No Hit
CTATTTGCGACA495350.313228393548412No Hit
GAGGAATAGCAG440710.2786774711228841No Hit
TTTTTTTTTTTT438570.2773242688170527No Hit
GGATCGCAGATC407900.257930476891889No Hit
TCCAAAGTGTTC393310.24870467238624389No Hit
GGAGACAAGGGA174850.11056421643674136No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGGG328250.06.0006331
TGATCCG2350.06.0006331
TTAGGTC1507.4578566E-116.0006331
GACCGGC751.7488468E-46.0006331
AAGCGGT751.7488468E-46.0006331
AGGCCAC2250.06.0006331
TCCCGGA2350.06.0006331
TAGGATC751.7488468E-46.0006331
GGAGACC600.00339258026.00063231
TCCGATG550.0091507616.00063231
CAGCCGT1001.2842029E-66.00063231
TCCCTGG550.0091507616.00063231
TCGGGGC550.0091507616.00063231
TCAGGGA1054.8213406E-76.00063231
GCACCTG550.0091507616.00063231
ACGTGCA1303.6379788E-96.00063231
CAGTGGT953.4237182E-66.00063231
TCCAACG704.6910509E-46.00063231
TCCCGGG2800.06.00063231
TCGAGGA180200.06.00063231
Produced by FastQC (version 0.11.2)