FastQCFastQC Report
Tue 20 Dec 2016
000000000-ARK0Y_l01n01.331000000746df.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-ARK0Y_l01n01.331000000746df.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15814339
Sequences flagged as poor quality0
Sequence length251
%GC48

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGGTGAACCTGCGGAGGGATCATTACTGAGTGAGGGAGAAATCCCGAC179089811.324520107985544No Hit
GTAGGTGAACCTGCGGAAGGATCATTAAAGAGAAACAAACGGCCCCTCGC6562304.149588547456837No Hit
GTAGGTGAACCTGCGGAAGGATCATTACCGAGTGCGGGTCCCTCGTGGCC5282813.3405190062006387No Hit
GTAGGTGAACCTGCGGAAGGATCATTAATGATTAACTGTCTGTCGAGCTT4655012.943537507321678No Hit
GTAGGTGAACCTGCGGAGGGATCATTACAAGTGACCCCGGTCTAACCACC3709802.3458457542866635No Hit
GTAGGTGAACCTGCGGAGGGATCATTACAAGTGACCCCGGTTTACCACCG2583041.6333531233901082No Hit
GTAGGTGAACCTGCGGAAGGATCATTACCGAGTGAGGGCCCTCTGGGTCC2348781.4852217345283922No Hit
GTAGGTGAACCTGCGGAAGGATCATTAGCGTATGATGATTGTGTGTGTGT2298961.4537186789786156No Hit
GTAGGTGAACCTGCGGAAGGATCAGTAGAGAATATTGGACTTCGGTCCAT2167721.3707307020546353No Hit
GTAGGTGAACCTGCGGAAGGATCATTAGTGAATATAGGACGTCCAACTTA2100561.3282629138024675No Hit
GTAGGTGAACCTGCGGAAGGATCATTAAAGAGTAAGGGTGCTCAGCGCCC1907201.2059941297578103No Hit
GTAGGTGAACCTGCGGAGGGATCATTACAAGTGACCCTGGCCTCCGGGCC1885111.1920257938064942No Hit
GTAGGTGAACCTGCGGAAGGATCATTACCGAGTGTTTGGGCGCCCCTTCT1547550.9785739384997374No Hit
GTAGGTGAACCTGCGGAGGGATCATTAACGAGTTAGGGTCTCACCGGCCC1437690.90910533788355No Hit
GTAGGTGAACCTGCGGAAGGATCATTAGTGAATCTAGGGTGTCCAATTTA1353560.8559067818136439No Hit
GTAGGTGAACCTGCGGAGGGATCATTACAAGTTGACCCCGGCCCTCGGGC1280570.8097524657843744No Hit
GTAGGTGAACCTGCGGAGGGATCATTACAAGAACGCCCGGGCTTCGGCCT1273000.8049656707118774No Hit
GTTGGTGAACCAGCGGAGGGATCATTACCGAGTTTACAACTCCCAAACCC1255610.7939693211331817No Hit
GTAGGTGAACCTGCGGAGGGATCATTACAAGAACTTCCGGGCTTCGGCCT1249240.7899413310919919No Hit
GTAGGTGAACCTGCGGAAGGATCATTAAAGAAATTTAATAATTTTGAAAA1207100.7632946277425823No Hit
GTAGGTGAACCTGCGGAAGGATCATTAATGAATGAAATTGAGTGGGGTTG1176470.7439261293184621No Hit
GTAGGTGAACCTGCGGAAGGATCATTTCCATAGCCAAAGATGCAAATCTT1171470.7407644416880149No Hit
GTAGGTGAACCTGCGGAAGGATCATTAATGAATACAGATGCCTGTCGAGC1104230.6982460664337599No Hit
GTAGGTGAACCTGCGGAAGGATCATTAATGAATTTAGATTGAACCATAGG1064830.6733319679058354No Hit
GTAGGTGAACCTGCGGAAGGATCATTACCTAGAGTTGTAGGCTTTGCCTG973330.6154730842686501No Hit
GTAGGTGAACCTGCGGAAGGATCATTACAGAATTTTGAGAATTGTGCTTA945580.5979257179196678No Hit
GTAGGTGAACCTGCGGAAGGATCATTAATGAATACACTGGACGCTCTTTT934060.5906411896191172No Hit
GTAGGTGAACCTGCGGAAGGATCATTAACGAATTTCTCAAAGTTGCTTAC921750.582857114672956No Hit
GTAGGTGAACCTGCGGAAGGATCATTACAGTTTAGGCGTGTGCTTAAGTG851570.538479667091998No Hit
GTAGGTGAACCTGCGGAGGGATCATTACACAATATGAAAGCGGGCTGGAA747180.47246995274351966No Hit
GTAGGTGAACCTGCGGTGGGATCATTACTGAGTGAGGGAGAAATCCCGAC729360.46120169802860556No Hit
GTAGGTGAACCTGCGGAAGGATCATTACAGTTATTACTTTCTACCAGCGC724090.45786927926611415No Hit
GTAGGTGAACCTGCGGAAGGATCATTACCGAGTGCGGGCCCTCTGGGTCC697930.441327329583614No Hit
GTAGGTGAACCTGCGGAAGGATCATTACTGAGTGCGGGCTGCCTCCGGGC638090.40348825202242095No Hit
GTAGGTGAACCTGCGGGAGGATCATTAATGATTAACTGTCTGTCGAGCTT634910.40147741868945647No Hit
GTAGGTGAACCTGCGGAAGGATCATTAACGAGTTTTTAATTGTTAGATTA590740.3735470701620852No Hit
GTAGGTGATCCTGCGGAGGGATCATTACTGAGTGAGGGAGAAATCCCGAC586210.37068258116889996No Hit
GTAGGTGAACCTGCGGAAGGATCATTAGCGAAGCTCGGAACACATGTTCG432230.27331524890164555No Hit
GTAGGTGAACCTGCGGAAGGATCATTAGAGAACCTGGGCCTGCGCTACTT375810.23763876567967843No Hit
GTAGGTGAACCTGCGGAAGGATCATTACTGAGTGAGGGCCCTCTGGGTCC366330.23164420593235038No Hit
GTTGGTGAACCAGCGGAGGGATCATTACAGAGTTATCCAACTCCCAAACC364280.230347914003867No Hit
GTAGGTGAACCTGCGGAAGGATCATTACAGAGAAACAAACGGCCCCTCGC332800.21044192868257092No Hit
GTAGGTGAACCTGCGGAAGGATCATTAGAGTTTGAGCCGGCGCTTGGGTG328680.20783669807508237No Hit
GTAGGTGAACCTGCGGAGGGATCATTACTGAGTGAGGGAGGAATCCCGAC316690.20025497113726978No Hit
GTAGGTGAACCTGCGGAAGGATCATTACTGAGTGCGGGTCCCTCGTGGCC311960.19726401463886667No Hit
GTAGGTGAACCTGCGGAAGGATCATTAGAATACTGTTTAACACTGTGTCT304310.19242663256428233No Hit
GTAGGTGAACCTGCGGAAGGATCATTAGTGATTAATTATTGAGTGTAAAA303770.192085170300194No Hit
GTAGGTGAACCTGCGGAAGGATCATTAAAGTTTGCTTAATTGCATTACAC297360.1880318867579606No Hit
GTAGGTGAACCTGCGGAAGGATCATTACTGAGTGAGGGCCCCTCGGGGTC278770.1762767321479576No Hit
GTAGGTGAACCTGCGGAGGGATCATTACAAGTGACCCCCGGCTCCGGCCG259010.16378174263242998No Hit
GTAGGTGAACCTGCGGAAGGATCATTATTGTAGGGACTCCGGTCCCCTCA256250.16203649106042306No Hit
GTAGGTGAACCTACGGAAGGATCATTAGTGAATCTAGGGTGTCCAATTTA235980.1492190094065898No Hit
GTAGGTGAACCTGCGGAAGGATCATTACTGATTAACTGTCTGTCGAGCTT227400.14379355343274228No Hit
GTAGGTGAACCTGCGGAAGGATCATTAATGAGAAACAAACGGCCCCTCGC226910.14348370804495844No Hit
GTAGGTGAACCTGCGGAAGGATCATTAATGAAATGTTAGGACGCTCTTTT206690.13069784326742964No Hit
GTAGGTGAACCTGCGGTAGGATCATTAATGATTAACTGTCTGTCGAGCTT193420.12230672429622257No Hit
GTAGGTGATCCTGCGGAAGGATCATTAATGATTAACTGTCTGTCGAGCTT174110.11009628666743516No Hit
GTAGTTGAACCTGCGGAGGGATCATTACTGAGTGAGGGAGAAATCCCGAC158450.10019388100887427No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGTGAA12018700.0244.691933
TAGGTGA12460500.0244.69012
ACCTGCG12604550.0244.544169
GTAGGTG12647700.0244.288851
TGAACCT12494300.0244.155616
GGTGAAC12448700.0243.958654
GAACCTG12513000.0243.193567
AACCTGC12581100.0242.434338
GTGAACC12935800.0237.028065
TGATCCT516700.0234.18986
GTGATCC509500.0233.868745
GGTGATC504650.0232.645144
GATCCTG519550.0232.315697
ATCCTGC527000.0230.541728
CGTCTTT1874750.0217.76541245
GGAGGTG113800.0211.307771
TTGGTGA320950.0210.3062
GGACCTG101150.0208.305157
GTTGGTG334850.0206.807421
AGGTGGA96850.0205.9173
Produced by FastQC (version 0.11.2)