FastQCFastQC Report
Tue 23 Aug 2016
000000000-ARG6P_l01n03.333000000677f9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-ARG6P_l01n03.333000000677f9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17294270
Sequences flagged as poor quality0
Sequence length251
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTATTGGCCCAGGG526204930.426545902197667No Hit
CCTGTTTGCTCCCCACGCTTTCGCACATGAGCGTCAGTACATTCCCAAGG16216249.376654811102174No Hit
CCTGTTCGCTACCCACGCTTTCGAGCCTCAGCGTCAGTTACAAGCCAGAG7881854.557492163589443No Hit
CCTGTTTGCTACCCACGCTTTCGAGCATGAACGTCAGTGTTATCCCAGGA5359083.0987604564980193No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGTGTCAGTTACAGGCCAAAA3791102.192113341586549No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAGCGTCAGTTGCAGCCCGGAC3559792.058363839583862No Hit
CCTGTTTGCTACCCACGCTTTCGCGCTTCAGCGTCAGTATCTGTCCAGTA3441731.990098454574839No Hit
CCTGTTTGCTCCCCATGCTTTCGTACCTCAGCGTCAGTATTAGGCCAGAT2507541.4499253220864483No Hit
CCTGTTTGCTACCCACGCTTTCGAACCTCAGTGTCAGTATGATGCCAGGA2248791.3003092931936415No Hit
CCCGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA2217251.282072038889181No Hit
CCTGTTTGATACCCACGCTTTCGTGCTTCAGCGTCAGTTGTACCTTAGTA2041431.1804083086478931No Hit
CCTGTTTGCTACCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG1916941.1084249291817463No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAAGCCAGAG1772721.0250331468168359No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCATGAGCGTCAGTATTGGCCCAGGG1370140.7922508437765803No Hit
CCTGTTTGCTACCCACGCTTTCGCGCTTTAGCGTCAGTATCTGTCCAGTA1169780.6763974426211687No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG968640.5600930250308339No Hit
CCTGTTCGCTCCCCACGCTTTCGTCCCTCAGCGTCAGTTATATCTTGGTA916620.5300136981786453No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTCGTCCAGGG908740.5254572757335233No Hit
CCTGTTTGCTCCCCTAGCTTTCGCACTTCAGCGTCAGTTACCGTCCAGTG865060.5002003553778217No Hit
CCTGTTCGCTCCCCCGGCTTTCGCACTTCAGCGTCAGTTACCGTCCAGTG842550.48718448364689576No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTGCAGGCCCAGGG620800.35896282410301217No Hit
CCTGTTTGCTCCCCACGCCTTCGAGCCTCAACGTCAGTTATCGTCCAGTA615250.3557536687006737No Hit
CCTGTTCGCTCCCCTAGCTTTCGCACTTCAGCGTCAGTTGCCGTCCAGTG578370.334428686495585No Hit
CCTGTTCGCTCCCCTAGCTTTCGCACTTCAGCGTCAGTTACCGTCCAGTG574410.33213891074905155No Hit
CCTGTTTGCTCCCCACGCTTTCGCACATGAGCGTCAGTACTTTCCCAAGG570640.3299589979802559No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTACAGGCCCAGGG558190.3227600818074426No Hit
CCCGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTTACCGTCCAGAA542360.3136067610832952No Hit
CCTGTTTGCTCCCCTAGCTTTCGCACTTCAGCGTCAGTTTCTGTCCAGTG525340.3037653511827906No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTGAGCGTCAGTATTGGCCCAGGG493450.2853257177088134No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTATTGTCCCAGGG480670.2779359868904556No Hit
CCCGTTCGCTCCCCTGGCTTTCGAGCCTCAGCGTCAGTTGCAGTCCAGAA456570.26400073550372466No Hit
CCTGTTTGCTACCCACGCTTTCGGGCATGAACGTCAGTGTTATCCCAGGA416980.24110876030037695No Hit
CCTGTTTGCTCCCCAAACTGTCGTCCCTCATCGTCAAGTATTCTATAGTT389160.22502250745478125No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGTGTCAGTATCAGTCCAGGT375960.21738992163300327No Hit
CCTTTTCGCTCCCCACGCTTTCGTTTTTCAGCGTCAATACAGTTCCAGTA373060.21571306565700663No Hit
CCTGTTCGCTCCCCACGCTTTCGTCCATCAGCGTCAATCAATTGTTAGTA341280.19733703706487754No Hit
CCTGTTTGCTCCCCACGCTCTCGTGCATGAGCGTCAGTATTGGCCCAGGG332840.19245680794852862No Hit
CCTGTTCGATACCCACGCTTTCGAGCATCAGCGTCAGTTGCGCTACAGTA328250.18980274969686492No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTATTGGTCCAGTT324940.18788882097943424No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGCGTCAGTTGTTGTCCAGAA314520.1818637039898186No Hit
ACTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTATTGGCCCAGGG304400.17601205485978882No Hit
CCTGTTCGATACCCGCACCTTCGAGCTTAAGCGTCAGTTACACTCCCGTA303010.17520832044370765No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGCGCGTCAGTATTGGCCCAGGG302620.17498281222624604No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCATGAGCGTCAGTATTGTCCCAGGG301980.17461274745912952No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCATGAGCGTCAGTACTTTCCCAGGG286330.16556350745073367No Hit
CCTGTTCGCTACCCACGCTTTCGCGCCTCAGCGTCAGTTACAAGCCAGAG283320.16382304659288888No Hit
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG282880.16356862706549624No Hit
CCTGTTTGATCCCCACGCTTTCGCACATCAGCGTCAGTTACAGACCAGAA270850.15661256589610317No Hit
CCTGTTCGCTCCCCACGCTTTCGTCCCTCAGCGTCAGTTGTATCTTAGTA266990.15438061276943174No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCATGAGCGTCAGTATTTTCCCAGGG251920.14566674395623522No Hit
CCTGTTCGCTCCCCATGCTTTCGCTCCTCAGCGTCAGTTACTGCCCAGAG243330.14069978091009336No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTATTGGCCCCGGG225680.13049408850445843No Hit
CCTGTTTGCTACCCACGCTTTCGAGCCTCAGCGTCAGTTACAAGCCAGAG220680.1276029575113607No Hit
CCTGTTTGCTACCCACGCTTTCGCGCATGAACGTCAGTGTTATCCCAGGA208380.12049077526834033No Hit
CCTGTTTGCTCCCCCAGCTTTCGCACTTCAGCGTCAGTTACCGTCCAGTG207990.1202652670508787No Hit
CCTGTTTGCTACCCACGCTTTCGTGCATGAGCGTCAGTATTGGCCCAGGG200580.11598061091910788No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTATTGGCCCAGTG199830.11554694127014324No Hit
CCTGTTCGATACCCGCACCTTCGAGCTTAAGCGTCAGTTGCGCTCCCGCC188900.10922692891923162No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCATGAACGTCAGTGTTATCCCAGGA186000.10755007294323496No Hit
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAAGCCAGAG185290.10713953234221507No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGAG183660.10619702363846523No Hit
CCTGTTTGCTCCCCACGCTTTCGCACATGAGCGTCAGTACATTCCCAGGG178600.10327119907345032No Hit
CCTGTTTGCTACCCACGCTTTCGAGCCTCAGCGTCAGTTTTGGTCCAGCA178580.10325963454947795No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTGTTATCCCAGGA174630.10097564106493076No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTTTGC12879050.0244.825933
TTTGCTC10782550.0244.801185
CTGTTCG2078750.0244.628742
GTTTGCT12916350.0244.461294
CTGTTTG13803600.0244.416062
TTGCTCC10832700.0244.071586
TTGATAC816200.0243.964426
TGTTCGA219500.0243.66063
CCTGTTT13732550.0243.457661
TGCTCCC10886900.0242.864367
CCTGTTC2077250.0242.812131
TGTTCGC1879300.0242.347023
TTTGCTA2171500.0242.106035
GCTCCCC12236650.0241.534238
TGTTTGA925000.0241.437563
TTGCTAC2185300.0241.204996
CTCCCCA11238250.0240.098139
GTTCGCT2334100.0239.352844
GTTTGAT934500.0239.179784
TGATACC837100.0237.697717
Produced by FastQC (version 0.11.2)