FastQCFastQC Report
Tue 23 Aug 2016
000000000-ARG6P_l01n02.332000000677fc.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-ARG6P_l01n02.332000000677fc.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17294270
Sequences flagged as poor quality0
Sequence length12
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACGGTTGTGAG2547111.4728057327658237No Hit
AGATGTTCTGCT2537871.467462922690579No Hit
GCACACACGTTA2440901.411392328210442No Hit
CAAGCATGCCTA2413831.395739745013811No Hit
CTTCGGCAGAAT2409241.3930856867621473No Hit
CTATTTGCGACA2402831.3893792568289958No Hit
ACGGGACATGCT2340781.3535003212046532No Hit
CCAGTGTATGCA2302821.3315508547050554No Hit
CGATCCGTATTA2297061.328220271801007No Hit
CTAACCTCCGCT2270661.3129551001574509No Hit
TTGCGCATACTA2267991.3114112362071368No Hit
GAGTGGTAGAGA2259581.3065483538767464No Hit
CGCAGCGGTATA2237461.2937579903632823No Hit
CAGGCGTATTGG2194961.2691833769219516No Hit
ACACCTGGTGAT2193791.2685068522695666No Hit
CACGCCATAATG2193291.268217739170257No Hit
ATCCCGAATTTG2193261.2682003923842984No Hit
ATCGATCTGTGG2185251.2635688005333559No Hit
GACTTTCCCTCG2175801.2581045629564012No Hit
GAAGTTGGAAGT2169311.2543518749273603No Hit
GACAGGAGATAG2149861.2431053753642103No Hit
ATTCCTGTGAGT2147981.2420183101108055No Hit
ACATTCAGCGCA2139321.2370108712307604No Hit
GAACTAGTCACC2131371.232413972951735No Hit
TCCAAAGTGTTC2117411.2243419352190061No Hit
TTCCTAGGTGAG2111921.221167473388585No Hit
ACGTGTACCCAA2093761.210666885621654No Hit
TAGCTCGTAACT2074871.1997441927297308No Hit
TCCTCTGTCGAC2059071.1906082187915419No Hit
GTCAATTGACCG2035151.1767770481205624No Hit
TAGGATTGCTCG2029431.1734695942644586No Hit
ATGTGCACGACT2008981.161644868502689No Hit
AAGGAGCGCCTT1994061.1530177336192855No Hit
CTAGCGAACATC1983681.1470157456776147No Hit
GGCCACGTAGTA1978711.1441419614704755No Hit
GTATGCGCTGTA1971651.1400596845082216No Hit
AATCCGTACAGC1970041.139128740328444No Hit
TACACGATCTAC1965361.1364226417189045No Hit
GCTGATGAGCTG1944161.1241642463081702No Hit
AATCAGTCTCGT1942171.1230135761729174No Hit
CCTGAACTAGTT1938591.1209435263818595No Hit
CCTCGTTCGACT1922531.1116572136320295No Hit
TTACTGTGCGAT1918461.109303833003648No Hit
GAACCAAAGGAT1900251.0987743339267861No Hit
TCACGGGAGTTG1889501.0925584022916262No Hit
ACACGTAAGCCT1884181.08948223891497No Hit
GGAGACAAGGGA1880311.0872445035263125No Hit
GCGATATATCGC1869031.080722112005884No Hit
GACTTGGTATTC1855021.072621162963224No Hit
GTTGGTCAATCT1844391.0664746184718985No Hit
ACGCGCAGATAC1835181.0611491551826124No Hit
GTCATATCGTAC1828731.0574195962015165No Hit
GAGGCTCATCAT1800991.0413796014518104No Hit
GAATCTTCGAGC1793761.037199026035791No Hit
TAGGAACTGGCC1779051.0286933186540974No Hit
CCACCTACTCCA1769561.023205952029198No Hit
GGATCGCAGATC1718450.9936528110177532No Hit
GCACGACAACAC1700830.9834644653980769No Hit
AGGCTACACGAC1696560.9809954395299714No Hit
AGCTGGAAGTCC1668880.9649901383521826No Hit
CAGCGGTGACAT1648630.9532810578301367No Hit
GGAAACCACCAC1598070.9240459412279326No Hit
GACGGAACCCAT1565890.9054386221563558No Hit
GTCTAATTCCGA1562120.9032587093875601No Hit
CTTGTGTCGATA1445730.8359589621302316No Hit
AAGAGATGTCGA1421400.821890718717818No Hit
TATCGTTGACCA1396000.8072037732728817No Hit
CAGTGCATATGC1384460.8005310429408121No Hit
TACAGATGGCTC1365040.7893018901636206No Hit
ATCGGCGTTACA1336520.7728108789789914No Hit
AGTAGAGGGATG1292310.7472474987380213No Hit
ATGAGACTCCAC1286770.7440441255976691No Hit
TGAGTCACTGGT1281950.7412570753203228No Hit
CGGAGCTATGGT914000.5284987455382621No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCACA550.0091608386.00000053
CGGGTCA909.1527545E-66.00000052
GGCGTGT550.0091608386.00000053
GGATCCG550.0091608386.00000051
CGTCTTG1950.06.00000055
AGGGTTA550.0091608386.00000052
CGGGGCA1101.8154606E-76.00000052
CCCAAGG953.429981E-66.00000054
ACAGCGG550.0091608386.00000052
AAGCTTC550.0091608386.00000052
GACAGGT1701.8189894E-126.01
AACCGGC650.00126199546.05
GGCAGAC1850.06.05
GCAAGTC600.00339662356.05
TGCCCGA852.445055E-56.04
GAATGCG751.7514097E-46.01
CGGCCGA1552.7284841E-116.04
CCGGCAG751.7514097E-46.03
GACGTTG5050.06.01
AGCCAAG650.00126199546.03
Produced by FastQC (version 0.11.2)