FastQCFastQC Report
Tue 23 Aug 2016
000000000-ARG6P_l01n01.331000000677ff.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-ARG6P_l01n01.331000000677ff.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17294270
Sequences flagged as poor quality0
Sequence length251
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA572279833.09071733007522No Hit
TACGGGGGGTGCGAGCGTTAATCGGAATAACTGGGCGTAAAGGGCACGCA8190034.735689913480014No Hit
TACGTAGGTCCCGAGCGTTATCCGGATTTATTGGGCGTAAAGCGAGCGCA8075904.669696957431565No Hit
TACGGAGGGTGCGAGCGTTAATCGGAATAACTGGGCGTAAAGGGCACGCA7555724.368915253433651No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCGCGCA7172314.147217546620933No Hit
TACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGAGCGCA6294033.639373040897361No Hit
TACGTATGTCACAAGCGTTATCCGGATTTATTGGGCGTAAAGCGCGTCTA4129962.388051071250767No Hit
TACGGAAGGTCCAGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA3607462.0859278824720557No Hit
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA3399151.9654775830376188No Hit
TACAGAGGGTGCGAGCGTTAATCGGATTTACTGGGCGTAAAGCGTGCGTA2683581.5517162620914327No Hit
TACAGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGAGCGTA2420641.39967746542641No Hit
TACGGAGGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA2272121.3137993104074355No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA2202121.2733234765040675No Hit
TACGTATGTCGCAAGCGTTATCCGGAATTATTGGGCATAAAGGGCATCTA2159231.2485233548452754No Hit
TACGTATGTCGCGAGCGTTATCCGGAATTATTGGGCATAAAGGGCATCTA1405020.8124193735844301No Hit
TACGGAGGGTGCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTCCGTA1267570.7329421825841738No Hit
TACGTAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA1153110.6667584118901809No Hit
TACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGCA1071830.6197601864663845No Hit
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCA1049480.6068368309272378No Hit
TACGGAAGGTCCGGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA1011010.584592469066344No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA821620.47508220930978873No Hit
TACGGGGGGTGCAAGCGTTAATCGGAATAACTGGGCGTAAAGGGCACGCA753140.4354852792283225No Hit
TACGTAGGGTGCGAGCGTTAATCGGCATTACTGGGCGTAAAGCGTGCGCA732780.42371259382442855No Hit
TACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGGGCGCA678560.392361169335277No Hit
TACGTAGGGTGCGAGCGTTAATCGGACTTACTGGGCGTAAAGCGTGCGCA609840.35262546496614194No Hit
TACGAAGGGGGCTAGCGTTGCTCGGAATCACTGGGCGTAAAGGGCGCGTA560310.323985921348516No Hit
TACGTATGTCGCAAGCGTTATCCGGAATTATTGGGCTTAAAGGGCATCTA528750.30573710252008324No Hit
TACGTAGGTGGCGAGCGTTGTCCGGAATCATTGGGCGTAAAGGGAGCGCA501950.2902406403970795No Hit
TACGGAGGATGCGAGCGTTATCCGGAATCATTGGGTTTAAAGGGTCCGTA454610.2628674121544303No Hit
TACGTAGGTCTCAAGCGTTATCCGGATTTACTGGGCGTAAAGTGTCCGTA445400.2575419488651443No Hit
TACGTAGGTGGCAAGCGTTATCCGGAATTATTGGGCGTAAAGCGCGCGTA406030.23477718342549297No Hit
TACGTAGGGTGCGAGCGTTGTCCGGAATTACTGGGCGTAAAGAGCTCGTA350040.20240229856478476No Hit
TACGGAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA337830.19534215667964014No Hit
TACGGAAGGTCCGGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGTGTA314770.18200826053947347No Hit
TACATATGGGGCGAGCGTTATCCGGAATTATTGGGTGTAAAGGGTGCGTA300530.1737743194711312No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCA293190.16953013917326373No Hit
TACGGAAGGTTCGGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA286540.16568493495244377No Hit
TACGTAGGTGGCGAGCGTTGTCCGGATTTATTGGGCGTAAAGGGAGTGTA283550.16395603861857136No Hit
TACGTATGTCACGAGCGTTATCCGGATTTATTGGGCGTAAAGCGCGTCTA265070.1532704184680822No Hit
TACGGAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGCA260650.15071465867018383No Hit
TACGGAGGATGCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA254170.1469677529031292No Hit
TACATATGTCTCGAGCGTTACCCGGAATCACTGGGTGTAAAGGGTTTGTA251180.14523885656925675No Hit
TACGGAGGGTGCGAGCGTTAATCGGAATAACTGGGCGTAAAGCGCACGCA233680.13511989809341476No Hit
TACAGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGTA232400.13437976855918174No Hit
TACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAACGCGTGCGCA225710.130511435290417No Hit
TACGGAAGGTTCTGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA225020.13011245921336953No Hit
TACGGAGGATGCGAGCGTTATCCGGAATCATTGGGTTTAAAGGGTCTGTA221330.1279788045404634No Hit
TACGAAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGTA220470.1274815300096506No Hit
TACGTAGGTCCCAAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA217260.12562542391208187No Hit
TACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTACAGCGTGCGCA190910.11038916357845692No Hit
TACGTAGGTTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA184300.10656708840558174No Hit
TACGGAAGGTCCAGGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA183400.10604668482682414No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTAT1108950.0244.779051
TAGGTCC1373300.0244.687795
GTGCGAG9751450.0244.61819
GTAGGGT7422700.0244.562654
GTCCCGA1349150.0244.382578
GGTCCCG1351550.0244.102697
TCACAAG505200.0244.078579
TACGGAG1969050.0244.054381
TACGTAG10646400.0244.026581
AGGTCCC1380850.0243.935426
TATGTCG532200.0243.918185
GTCACAA506150.0243.765698
TACAGAG632350.0243.740811
ACGTAGG10627350.0243.528032
GTAGGTC1509450.0243.514864
TAGGGTG7455100.0243.445565
CGAACCG135350.0243.28038245
TGTCACA505100.0243.181057
CGAACAG537400.0243.08522245
GGTCCAG482700.0243.04598
Produced by FastQC (version 0.11.2)