FastQCFastQC Report
Fri 10 Feb 2017
000000000-ARD6C_l01n03.3330000007a81a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-ARD6C_l01n03.3330000007a81a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15519830
Sequences flagged as poor quality0
Sequence length251
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTATTGGCCCAGGG250244616.124184349957442No Hit
CCTGTTTGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGACCCAGAG10104916.510966937137843No Hit
CCTGTTTGCTCCCCACGCTTTCGCACATGAGCGTCAGTACATTCCCAAGG9045185.828143736110512No Hit
CCTGTTTGCTCCCCATGCTTTCGTATCTCAGTGTCAGTATCAGGCCAGAA4081762.6300288083052457No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTCGTCCAGGG3982582.566123469135938No Hit
CCTGTTTGCTACCCACGCTTTCGCGCTTCAGCGTCAGTATCTGTCCAGTA3017271.944138563373439No Hit
CCTGTTTGATACCCACGCTTTCGTGCTTCAGCGTCAGTTGTACCTTAGTA2745621.7691044296232625No Hit
CCTGTTTGCTACCCACGCTTTCGAGCATGAACGTCAGTGTTATCCCAGGA2683721.7292199721259833No Hit
CCTGTTCGCTACCCACGCTTTCGAGCCTCAGCGTCAGTTACAAGCCAGAG2661271.7147546074924789No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCATGAGCGTCAGTATTGGCCCAGGG1686261.0865196332691789No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGTGTCAGTTACAGGCCAAAA1621471.0447730419727537No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAGCGTCAGTTGCAGCCCGGAC1442450.9294238403384574No Hit
CCTGTTTGCTACCCACGCTTTCGCGCTTTAGCGTCAGTATCTGTCCAGTA1118440.7206522236390476No Hit
CCTGTTTGCTACCCACGCTTTCGAACCTCAGTGTCAGTATGATGCCAGGA1106600.7130232740951415No Hit
CCTGTTTGCTACCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG916110.5902835275901862No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAAGCCAGAG910670.5867783345564996No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG784240.5053148133710227No Hit
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG555980.3582384600862252No Hit
CCCGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA545510.35149225217028796No Hit
CCTGTTTGCTCCCCAAACTGTCGTCCCTCATCGTCAAGTATTCTATAGTT500130.3222522411650127No Hit
CCTTCTCGCTACCCAAGCCTTCGTCCCTCAACGTCAGTTTTTACATAGAA496490.3199068546498254No Hit
CCTGTTTGATCCCCACGCTTTCGCACATCAGCGTCAGTTACAGACCAGAA475390.3063113449051955No Hit
CCTGTTTGCTCCCCACGCTTTCGCACATGAGCGTCAGTACTTTCCCAAGG472920.3047198326270326No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTATTGGCCCCGGG431080.2777607744414726No Hit
CCCGTTCGCTCCCCTGGCTTTCGAGCCTCAGCGTCAGTTGCAGTCCAGAA413560.2664719909947467No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATCAGCGTCAGTATTGGCCCAGGG396600.25554403624266503No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTGTTATCCCAGGA352370.2270450127353199No Hit
CCTGTTTGCTACCCACGCTTTCGAGCATGAACGTCAGTATTATCCCAGGG350490.2258336592604429No Hit
CCTGTTCGCTCCCCCGGCTTTCGCACTTCAGCGTCAGTTACCGTCCAGTG331340.2134946065775205No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGAG297980.19199952576800133No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTGAGCGTCAGTATTGGCCCAGGG291780.1880046366487262No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTATTGTCCCAGGG289990.18685127349977415No Hit
CCTGTTTGCTACCCACGCTTTCGGGCATGAACGTCAGTGTTATCCCAGGA273370.1761423933122979No Hit
CCTGTTCGATACCCGCACCTTCGAGCTTAAGCGTCAGTTACACTCCCGTA265090.1708072833272014No Hit
CCTGTTCGCTCCCCACGCTTTCGTCCATCAGCGTCAATCAATTGTTAGTA255630.1647118557355332No Hit
CCTGTTTGCTACCCACGCTTTCGCACCTCAGCGTCAGTGTCAGTCCAGAA252720.16283683519729275No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGCGCGTCAGTATTGGCCCAGGG242090.15598753336860002No Hit
CCTGTTTGCTCCCCACGCCTTCGAGCCTCAACGTCAGTTATCGTCCAGTA238170.1534617325060906No Hit
CCTGTTTGCTCCCCACGCTTTCGCACATCAGCGTCAGTACATTCCCAAGG219210.1412451038445653No Hit
CCTGTTCGATACCCGCACCTTCGAGCTTAAGCGTCAGTTGCGCTCCCGCC214660.1383133707005811No Hit
CCTGTTTGCTACCCACGCTTTCGCGCATGAACGTCAGTGTTATCCCAGGA202550.13051045017890014No Hit
CCTGTTTGATCCCCACGCTTTCGCACATCAGCGTCAGTTGCAGACCAGAA200680.1293055400735704No Hit
CCTGTTTGCTCCCCATGCTTTCGCACCTCAGCGTCAGTATTAGGCCAGAA199530.1285645525756403No Hit
CCTGTTTGCTCCCCACGCTCTCGTGCATGAGCGTCAGTATTGGCCCAGGG193550.12471141758640399No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCATGAGCGTCAGTATTGTCCCAGGG185920.11979512662187665No Hit
CCCGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTTACCGTCCAGAA173880.1120373096870262No Hit
CCTGTTCGCTCCCCTAGCTTTCGCACTTCAGCGTCAGTTACCGTCCAGTG166430.10723699937434882No Hit
CCTGTTCGCTACCCACGCTTTCGCGCCTCAGCGTCAGTTACAAGCCAGAG166110.10703081154883785No Hit
CCTGTTTGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGACCCCGAG164450.10596121220399965No Hit
CCTGTTTGCTCCCCATGCTTTCGCATCTCAGTGTCAGTATCAGGCCAGAA158580.1021789542797827No Hit
CCTGTTCGCTCCCCATGCTTTCGCTTCTCAGCGTCAGTTACAGCCCAGAG157330.10137353308638046No Hit
CCTGTTTGCTCCCCTAGCTTTCGCACTTCAGCGTCAGTTACCGTCCAGTG156300.10070986602301701No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTTCG1136200.0285.789762
CTGTTTG10583800.0285.526922
CCTGTTT10605400.0283.950961
CCTGTTC1143250.0283.062321
CCGTTCG185850.0269.776282
ACTGTTT68000.0259.82241
CTATTTG23150.0251.921842
TGTTTGC11501500.0244.584353
TTTGCTC9834850.0244.480075
GTTTGCT11563200.0244.0394
TTGCTCC9920350.0243.44236
TTGATAC731950.0243.019416
TGTTCGA201100.0242.821613
TGCTCCC9991050.0241.969457
TGTTTGA894650.0241.370773
TGTTCGC1148650.0240.894333
TTTGCTA1820550.0240.406485
TTGCTAC1837400.0239.726756
TTTGATC167400.0237.691045
CTCCCCA10617900.0237.435359
Produced by FastQC (version 0.11.2)