FastQCFastQC Report
Fri 10 Feb 2017
000000000-ARD6C_l01n02.3320000007a81d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-ARD6C_l01n02.3320000007a81d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15519830
Sequences flagged as poor quality0
Sequence length12
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAAGGAGGTGA3391712.185404092699469No Hit
TAGCTCGTAACT1995671.2858839304296503No Hit
AGGCATCTTACG1759201.1335175707465868No Hit
CTAACCTCCGCT1519800.9792633037861884No Hit
TCAACAGCATCG1440160.9279483087121444No Hit
GCCTGAATTTAC1423860.9174456163501791No Hit
ACGTGTACCCAA1360390.8765495498339866No Hit
CACGCCATAATG1360210.8764335691821368No Hit
TAACACCACATC1336790.8613431977025521No Hit
AGTAGAGGGATG1304600.8406019911300575No Hit
ATCCCGAATTTG1287450.8295516123565787No Hit
AGAGCCTACGTT1249430.8050539213380559No Hit
GGTCACTGACAG1195570.7703499329567398No Hit
AACACAAGGAGT1184460.7631913493897807No Hit
CGGACTACAACT1174280.7566319991907128No Hit
CTAGATTTGCCA1167670.7523729319200017No Hit
ACATTCAGCGCA1145070.737810916743289No Hit
CCAAGTCTTACA1133800.7305492392635744No Hit
GACTCGAATCGT1124690.7246793296060589No Hit
AAGATGGATCAG1091930.7035708509693728No Hit
CAGCGGTGACAT1083460.698113316962879No Hit
ACGGGACATGCT1077680.6943890493645871No Hit
CTCTACCTCTAC1054900.6797110535360246No Hit
TAAACCGCGTGT1037160.6682805159592599No Hit
TATCGTTGACCA1031500.6646335687955345No Hit
GTCATATCGTAC1024030.6598203717437626No Hit
TCTGTTGCTCTC1013990.6533512287183558No Hit
CGGGTTTGACGA1013960.6533318986097141No Hit
CTACCGGATCAA1006300.6483962775365452No Hit
GAGGAATAGCAG998890.6436217407020567No Hit
GACACATTTCTG981430.6323716174726141No Hit
ACCACATACATC980320.6316564034528729No Hit
AGTTCCCGAGTA972340.6265145945541929No Hit
AGCCGGCACATA966150.622526148804465No Hit
TCCTCTGTCGAC962040.6198779239205584No Hit
GGATCGCAGATC960470.6188663149016451No Hit
CCTCTCGTGATC951800.6132799135042072No Hit
TACACGATCTAC927220.5974421111571454No Hit
GTCCGAAACACT918510.5918299362815185No Hit
AGCCTAAGCACG917950.5914691075868743No Hit
GACTTTCCCTCG914790.5894330028099535No Hit
AATGGAGCATGA912820.5881636590091516No Hit
GAATCTTCGAGC909080.585753838798492No Hit
TATGCACCAGTG905550.583479329348324No Hit
TCGGAGTGTTGT901280.5807280105516619No Hit
ATACTTCGCAGG896130.5774096752348447No Hit
TCTACGGAGAGC895820.5772099307788809No Hit
CGATCCGTATTA891130.5741879904612357No Hit
TGCAATGTTGCT890850.5740075761139136No Hit
ACGATGCGACCA885940.5708438816662296No Hit
TCGTCGATAATC882770.5688013335197615No Hit
TGGCAAGACTCT881780.568163439934587No Hit
TGGCACCGATTA878230.5658760437453245No Hit
CTTACACCAAGT871700.5616685234309912No Hit
ATGGACCGAACC856600.551939035414692No Hit
GGTCAGCTTAAC837300.5395033321885613No Hit
GCGGCAATTACG835080.5380729041490789No Hit
CAGTGCATATGC829920.5347481254627146No Hit
CTAGCGAACATC828270.5336849694874235No Hit
CAAGCATGCCTA824540.5312815926463113No Hit
TGACCTCCAAGA821110.5290715168916155No Hit
ATGTGGGACCCA818770.5275637684175665No Hit
GCATGGCTCTAA804770.5185430510514613No Hit
CTGCTAACGCAA803290.517589432358473No Hit
ACGCCACGAATG802480.5170675194251484No Hit
GCTTCGGTAGAT792920.5109076581380079No Hit
ATGTCGAGAGAA792160.5104179620524194No Hit
GTCTACACACAT791050.5097027480326782No Hit
CGTGACAATGTC786430.5067259113018635No Hit
CGAGAAGAGAAC767850.4947541306831325No Hit
GGCCACGTAGTA756660.48754400015979554No Hit
GAATGATGAGTG754160.48593315777299106No Hit
TGTTATCGCACA750120.48333003647591505No Hit
GAACTAGTCACC747120.4813970256117496No Hit
CAACACGCACGA735900.47416756497977103No Hit
GATTGGTTGCAC733590.47267914661436367No Hit
TAATCCACAGCG731880.47157733042178945No Hit
ACGGCATGGCAT731790.47151934009586444No Hit
ATACCTTCGGTA719340.46349734500957807No Hit
GTACGTGGGATC715910.4612872692548823No Hit
ATGGTTGTTGGC705990.4548954466640421No Hit
CCATAGGGTTCA686060.4420538111564366No Hit
TAGCGGATCACG685620.44177030289635905No Hit
TCCGAATTCACA682800.43995327268404355No Hit
AATCAGTCTCGT671220.4324918507483652No Hit
GCTCAGTGCAGA668970.43104209260024107No Hit
TGTAATTGTCGC667780.4302753316241222No Hit
TTACTGTGCGAT657550.42368376457731816No Hit
TCTTCCGCTACT653360.42098399273703385No Hit
ATCGCTCGAGGA648320.417736534485236No Hit
AGCTTGACAGCT643010.41431510525566323No Hit
TACAGATGGCTC631820.4071049747323263No Hit
GAAGTTGGAAGT627530.4043407691965698No Hit
CTTCGGCAGAAT624170.4021757970287046No Hit
TACTTCGCTCGC624020.40207914648549625No Hit
ACTCACGGTATG623680.4018600719208909No Hit
CACGGTTGTGAG614160.3957259841119394No Hit
GTCTAATTCCGA611550.3940442646601155No Hit
CCATGCGATAAC608450.39204682010047787No Hit
GCAGGATAGATA598450.3856034505532599No Hit
CGCAGCGGTATA594470.38303898947346715No Hit
AGGCTACACGAC570690.36771665669018283No Hit
CGACTGTCTTAA568650.36640220930255035No Hit
AATGCCTCAACT564180.3635220231149439No Hit
GCTGATGAGCTG552710.3561314782442849No Hit
ATGTGCACGACT547280.3526327285801455No Hit
GTTGGTCAATCT544510.35084791521556613No Hit
TCAAGCTCAAGC541370.34882469717773973No Hit
CGTCCGAAATAC536960.34598317120741656No Hit
CGCCAAATAACC534470.3443787721901593No Hit
GTACATACCGGT533320.3436377846922292No Hit
ACACCTGGTGAT528360.3404418733968091No Hit
GTCAATTGACCG516210.3326131793969393No Hit
ATTGGGCTAGGC507630.32708476832542627No Hit
TTCCTAGGTGAG503030.324120818333706No Hit
TGAGCCGGAATC497160.32033856040948905No Hit
TCCAAAGTGTTC495250.3191078768259704No Hit
CCACCTACTCCA486380.31339260803758806No Hit
TCGAGGACTGCA471250.30364378991264723No Hit
TTATGCAGTCGT470500.3031605371966059No Hit
TTGATGCTATGC470150.30293501926245325No Hit
TAACGCTTGGGT467790.3014143840493098No Hit
TATCAGGTGTGC457110.29453286537288104No Hit
AAGAGATGTCGA456950.2944297714601255No Hit
ACTATTGTCACG454280.2927093917910183No Hit
AATCTTGCTGCA449830.28984209234250635No Hit
TCCCAGAACAAC442880.2853639505071898No Hit
ACTGACAGCCAT433710.27945538063239095No Hit
GTATGCGCTGTA428420.2760468381419126No Hit
GTACCTAATTGC419700.27042821989673854No Hit
AGCTGTTGTTTG411170.26493202567296165No Hit
CCTGAACTAGTT404970.2609371365536865No Hit
GACAGGAGATAG384940.24803106735060884No Hit
AATGTCCGTGAC384210.24756070137366193No Hit
GAAGAAGCGGTA369250.23792142053102386No Hit
AGATGTTCTGCT364800.23505412108251184No Hit
CAGGCGTATTGG364010.23454509488828162No Hit
ACGCGCAGATAC343080.2210591224259544No Hit
TCCGACACAATT341980.22035035177576046No Hit
CTCGAGAGTACG338160.2178889846087232No Hit
CCTCGTTCGACT325610.2098025558269646No Hit
TAGGAACTGGCC312710.2014906091110534No Hit
TTAGGGCTCGTA312200.20116199726414527No Hit
TCTAGCGTAGTG308440.1987392903143913No Hit
GCGATATATCGC297120.19144539598694058No Hit
AGCTGGAAGTCC278040.1791514468908487No Hit
TAGGATTGCTCG272870.17582022483493698No Hit
CTTGTGTCGATA266750.17187688267203957No Hit
CCAGTGTATGCA261180.16828792583423918No Hit
GCACACACGTTA259560.16724409996758985No Hit
AATCCGTACAGC252390.16262420400223457No Hit
GACGGAACCCAT251000.16172857563517126No Hit
GGATGGTGTTGC241300.1554785071743698No Hit
GACTTGGTATTC240310.15484061358919524No Hit
CAGGAAGGTTAA227990.14690238230702268No Hit
ATGAGACTCCAC224400.14458921263957145No Hit
CAGCTAGAACGC223480.1439964226412274No Hit
GAACCAAAGGAT190480.12273330313540805No Hit
GGAGACAAGGGA186950.1204587936852401No Hit
ATCGATCTGTGG168690.10869320089202009No Hit
GAGTGGTAGAGA166530.10730143306982098No Hit
ATTAGTTCGCGT160980.10372536297111501No Hit
CCATACATAGCT160580.10346762818922631No Hit
GAATAGAGCCAA160210.10322922351597924No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAGGTG550.00916076.02
TGAATAG751.751361E-46.03
GGAGGTA650.00126196846.05
ATCTCAG1054.8307993E-75.99999953
GCTGAGT1156.827213E-85.99999955
TGGCAAG92550.05.9967591
GTCTAAT70150.05.99572371
TCTACGG95950.05.9937471
GTATGCG45250.05.99337051
CCATAGG75050.05.99200531
TTACTGT72150.05.9916841
GTAGAGG139600.05.9914042
AGAGCCT134500.05.99107841
GGTCAGC89150.05.9899051
TAAACCG117150.05.98975661
ACGTGTA142650.05.9894851
CATTCAG120900.05.9875932
AATCAGT72200.05.98753451
AATGGAG94600.05.98731471
GGCATCT182750.05.9868682
Produced by FastQC (version 0.11.2)