FastQCFastQC Report
Fri 10 Jun 2016
000000000-AR0HW_l01n03.33300000058bd9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AR0HW_l01n03.33300000058bd9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13526393
Sequences flagged as poor quality0
Sequence length251
%GC52

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTTGCTCCCCACGCTTTCGCACATGAGCGTCAGTACATTCCCAAGG183992013.60244375570043No Hit
CCTGTTTGCTCCCCACGCTTTCGCACATGAGCGTCAGTATATTCCCAAGG6889135.093102056106162No Hit
CCTGTTTGATCCCCACGCTTTCGCACATCAGCGTCAGTTACAGACCAGAA6823625.04467081505025No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTCGTCCAGGG4755383.5156305158367056No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGTCCAGAG4096923.0288340727642615No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTATTGGCCCAGGG2573141.9023105420639488No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTTACAGTCCAGAG1899351.4041807006494635No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTTGTCCAGGG1837101.358159562567789No Hit
CCTGTTTGCTACCCACGCTTTCGGGCATGAACGTCAGTATTATCCCAGGG1690221.2495718555567623No Hit
CCTGTTCGCTCCCCACGCTTTCGTCCATCAGCGTCAATAATTTGTTAGTA1569361.1602206146161804No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAAGCCAGAG1390881.0282711732536531No Hit
CCTGTTTGCTACCCACGCTTTCGCGCTTTAGCGTCAGTATCTGTCCAGTA1225610.9060878240045221No Hit
CCTGTTTGCTCCCCACGCTTTCGTTCCTCAGTGTCAATATACAACCAGTT1137460.8409189352993072No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGTGTCAGTATCAGTCCAGGT1120170.8281365179911601No Hit
CCTGTTTGATACCCACGCTTTCGTGCATCAGCGTCAGTTGTAGCCTAGTA1048350.7750403230188565No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTAATGTCCAGCA972210.7187503719579935No Hit
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGTCCAGAG768270.5679784699439089No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTTATGGTCCAGAA762060.5633874455666045No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTACAGTCCAGAA707260.5228740581469132No Hit
CCTGTTCGCTCCCCACGCTTTCGTCCATCAGCGTCAGTTGTAACTTGGTA623840.46120203664051457No Hit
CCCATTCGCTCCCCTAGCTTTCGTCTCTCAGTGTCAGTGTCGGCCCAGCA612430.45276667623068473No Hit
CCTGTTTGCTCCCCACGCTTTCGCACATGAGCGTCAGTACACTCCCAAGG598650.44257918574449223No Hit
CCTGTTCGCTACCCATGCTTTCGCTCCTCAGCGTCAGTAACTGCCCAGTA572800.42346839989049556No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG565050.4177388606112509No Hit
CCTGTTTGCTACCCACGCTTTCGCGCTTCAGCGTCAGTATCTGTCCAGTA563960.4169330286352023No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGTCCAGAA559410.41356923460674255No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACAGTCCAGAG500990.37037959787210084No Hit
CCTGTTCGCTACCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG446770.33029500177911436No Hit
CCTGTTTGATACCCACGCTTTCGTGCTTCAGTGTCAGTTGCGGTTTGGTA417140.308389679347628No Hit
CCTGTTTGCTACCCACGCTTTCGAGCATGAACGTCAGTGTTATCCCAGGA416920.30822703436163656No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTTTCAGTCCAGAA410420.30342161432097975No Hit
CCTGTTTGCTCCCCACGCTTTCGCACATCAGCGTCAGTTACAGACCAGAA393890.291201061509894No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTGTTATCCCAGGG371680.27478131087866514No Hit
CCTGTTTGCTCCCCATGCTTTCGTACCTCAGCGTCAGTATTAGGCCAGAT358420.2649782539957252No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTATCTGTCCAGAA344110.25439893695237153No Hit
CCTGTTTGCTCCCCATGCTTTCGTACCTCAGCGTCAGTGTTAGGCCAGAT314010.23214614568717618No Hit
CCTGTTCGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTTACAGTCCAGAA301290.22274230831530623No Hit
CCTGTTCGCTCCCCATGCTTTCGCTCCTCAGCGTCAGTTACTGCCCAGAG284980.210684400490212No Hit
CCTGTTTGCTACCCACGCTTTCGAACCTCAGTGTCAGTATGATGCCAGGA272600.20153192355123795No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTGTTATCCCAGGA270010.19961714848888393No Hit
CCTGTTCGCTCCCCACGCTTTCGTGACTCAGCGTCAGTTTCAGTCCAGTA264270.19537359294528853No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTACAGGTCCAGGG251870.18620633009849707No Hit
CCTGTTTGCTCCCCACGCTCTCGCACATGAGCGTCAGTACATTCCCAAGG249200.18423241140487343No Hit
CCCATTTGCTCCCCTAGCTTTCGTCTCTCAGTGTCAGTGTCGGCCCAGCA244300.18060986398960904No Hit
CCTGTTCGCTCCCCACGCTTTCGTCCCTCAGCGTCAGTTATAACTTGGTA239390.176979923620436No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGAG227270.16801966348308822No Hit
CCTGTTCGCTACCCATGCTTTCGCTCCTCAGCGTCAGTTACTACCCAGAG215800.15953994534980612No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTGATCGTCCAGTA215150.15905940334574042No Hit
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG212690.15724073668419955No Hit
CCCGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTTGTCGTCCAGAA203900.15074233019844982No Hit
CCTGTTTGCTCCCCACGCTTTCGCACATCAGCGTCAGTACATTCCCAAGG203800.15066840065936277No Hit
CCTGTTCGCTCCCCATGCTTTCGCTTCTCAGCGTCAGTTACAGCCCAGAG197640.14611434105160187No Hit
CCTGTTTGCTACCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG189240.13990425976829152No Hit
CCTGTTCGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGCAGACCAGAC188620.13944589662595194No Hit
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAATGTTAGACCAGAA181750.13436693729067314No Hit
CCTGTTCGCTCCCCATGCTTTCGCTCCTCAGCGTCAGTAACAGCCCAGAG171670.12691483975070073No Hit
CCTGTTCGCTACCCATGCTTTCGCTCCTCAGCGTCAGTTACTGCCCAGAG166810.12332186415107116No Hit
CCTGTTCGCTCCCCTAGCTTTCGCACTTCAGCGTCAGTTATCGTCCAGTG164170.12137012431917363No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGTGTCAGTTACAGGCCAAAA161230.11919659587001502No Hit
CCTGTTTGCTCCCCACGCTTTCGCACATGAGCGTCAGTATCTTCCCAAGG160400.11858298069559267No Hit
CCTGTTTGCTACCCACGCTTTCGCGCATGAACGTCAGTATTATCCCAGGG145580.10762662300289516No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCATGAGCGTCAGTATTGGCCCAGGG140360.10376750106255231No Hit
CCTGTTTGCTCCCCACGCTTTCGCACATGAGCGTCAGTACCTTCCCAAGG138820.10262898616061207No Hit
CCTATTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTTACAGACCAGGT136100.10061810269744492No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTTTGC7442700.0284.838653
TTTGCTC6815300.0284.709325
TGTTTGA1070000.0284.246733
TTTGATC844000.0283.78455
GTTTGCT7478400.0283.694344
TTGATAC236450.0282.31886
CTCCCCA7860850.0280.618449
GCTCCCC8022600.0280.46468
GATCCCC870350.0279.828868
TGATCCC864350.0279.231057
TGTTCGC1159700.0278.501653
TTGATCC870500.0277.307436
GTTTGAT1104300.0275.314644
TTGCTCC7124500.0274.766546
TGTTCGA24700.0273.60823
TGCTCCC7159200.0273.558267
ATTCGCT97450.0266.86444
TTTGCTA788650.0266.351145
ATCCCCA917600.0265.528389
GTTCGCT1273050.0265.463934
Produced by FastQC (version 0.11.2)