FastQCFastQC Report
Fri 10 Jun 2016
000000000-AR0HW_l01n02.33200000058bdc.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AR0HW_l01n02.33200000058bdc.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13526393
Sequences flagged as poor quality0
Sequence length12
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGTGTCGATA205444415.188409800010986No Hit
ACGCCACGAATG5374543.973372650047947No Hit
CAGGCGTATTGG4670203.452657334442375No Hit
ACACCTGGTGAT4054392.997391839790549No Hit
AAGAGATGTCGA3670112.713295406986918No Hit
TACAGATGGCTC3417752.5267268221468946No Hit
CTAACCTCCGCT3288592.4312394294620896No Hit
ACGGGACATGCT3054512.2581851643671746No Hit
AGTAGAGGGATG2982112.2046601780681665No Hit
TCCAAAGTGTTC2874442.1250602433331633No Hit
TTGCGCATACTA2759042.0397455552267334No Hit
ACGTGTACCCAA2367901.7505775560417325No Hit
GAGGAATAGCAG2163491.5994581851939391No Hit
AGATGTTCTGCT2038161.506802293856167No Hit
GGTGTCTATTGT2032871.5028914212384632No Hit
GTTGGTCAATCT1981101.4646180988531088No Hit
CGCAGCGGTATA1722801.273658099391316No Hit
TTACTGTGCGAT1670821.2352295249738787No Hit
CAGCTAGAACGC1273780.9416996829827434No Hit
AAGGAGCGCCTT1252150.9257087236782193No Hit
TAGCTCGTAACT1204070.8901634012851763No Hit
GACGGAACCCAT1203760.8899342197140065No Hit
ATCCCGAATTTG1166530.862410252311906No Hit
GGATCGCAGATC962760.7117640305142694No Hit
GAAGTTGGAAGT961280.7106698733357814No Hit
GTCTAATTCCGA903980.6683082474389144No Hit
TCCGAATTCACA892930.6601390333697978No Hit
TAGGAACTGGCC865090.6395570496879692No Hit
GAGGCTCATCAT829840.613496887159792No Hit
CACGCCATAATG775420.5732644319886314No Hit
TTAGGGCTCGTA733490.5422657762494406No Hit
ATCGATCTGTGG705190.5213437166878118No Hit
GAACCAAAGGAT663730.49069252978233No Hit
TTCCTAGGTGAG652790.4826046382062092No Hit
GCTGATGAGCTG636700.4707093753671064No Hit
TCGAGGACTGCA636430.4705097656115714No Hit
CCAGTGTATGCA610190.4511106545551353No Hit
CGGAGCTATGGT593530.4387939933432365No Hit
CTTCGGCAGAAT588710.4352305895592417No Hit
AGGCATCTTACG587130.43406250284166664No Hit
CCTCGTTCGACT584580.43217729959494744No Hit
ATGAGACTCCAC576510.42621118579062434No Hit
TCCTCTGTCGAC566960.4191509148078132No Hit
ACGCGCAGATAC564990.41769450288779875No Hit
ATTCCTGTGAGT563040.4162528768756017No Hit
GACTTTCCCTCG559430.41358402051455995No Hit
GAACTAGTCACC542170.40082378206813896No Hit
GTCAATTGACCG523880.38730206936912154No Hit
GGCCACGTAGTA514200.38014568998549725No Hit
TAGGATTGCTCG505950.37404650301081743No Hit
ACACGTAAGCCT455410.33668251395623355No Hit
CTAGCGAACATC455020.3363941887537942No Hit
GTACATACCGGT451950.3341245519038224No Hit
GGAGACAAGGGA412020.3046044869463722No Hit
TGAGTCACTGGT410130.30320721865762734No Hit
TCCCAGAACAAC409420.30268231893010944No Hit
CAGCGGTGACAT404960.29938506148682803No Hit
GACAGGAGATAG403490.29829829726224866No Hit
GAGTGGTAGAGA398500.294609213261806No Hit
AGGCTACACGAC376270.27817467672275975No Hit
AATCAGTCTCGT356960.26389888272505463No Hit
ATGTGCACGACT351880.2601432621394336No Hit
GACTTGGTATTC342730.2533787093129706No Hit
GAATCTTCGAGC338820.25048806433466775No Hit
CACGGTTGTGAG316560.2340313489338954No Hit
CCTGAACTAGTT298880.22096060642330886No Hit
AGCTGTTGTTTG269300.19909224876136605No Hit
AATCCGTACAGC258080.1907973544758015No Hit
CAAGCATGCCTA216850.1603162055102199No Hit
AGCTGGAAGTCC212310.15695980443566884No Hit
TCTAGCGTAGTG208340.15402480173391384No Hit
GCGGCAATTACG206750.1528493220624301No Hit
GCGATATATCGC206510.15267189116862123No Hit
CCACCTACTCCA203010.15008435730057526No Hit
CTGCTAACGCAA188660.13947546844158676No Hit
GTATGCGCTGTA164960.12195416767796115No Hit
GCACGACAACAC164130.12134055250353883No Hit
GCACACACGTTA140980.10422586420489187No Hit
CTTGTGGCGATA140360.10376750106255231No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGGGC650.0012619296.00000054
CGGGCTG650.0012619296.00000056
TGCAGCG1451.9645086E-106.00000051
TGCGTCG5800.06.00000053
CATGGTG600.0033964716.04
GTGCACC1101.8151877E-76.03
TCTGAGG550.0091604936.06
AGTCCGA550.0091604936.06
CCGGGAC1800.06.01
CAACGTG2750.06.03
CCTAGGC806.5390705E-56.03
CCTAGGA550.0091604936.03
CGTGCAC953.4296118E-66.02
CCAGGAA2350.06.04
GACGTCG2100.06.05
TAGCGGG1900.06.03
CGCGCCG909.151867E-66.02
TTGAGGG704.6972113E-46.03
TGGTTCG909.151867E-66.03
GGCCATG1101.8151877E-76.04
Produced by FastQC (version 0.11.2)