FastQCFastQC Report
Fri 10 Jun 2016
000000000-AR0HW_l01n01.33100000058bdf.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AR0HW_l01n01.33100000058bdf.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13526393
Sequences flagged as poor quality0
Sequence length251
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACGGAGGGTGCGAGCGTTAATCGGAATAACTGGGCGTAAAGGGCACGCA247870318.32493703236332No Hit
TACGGAGGGTGCGAGCGTTAATCGGAATGACTGGGCGTAAAGGGCACGCA8997146.651544133014618No Hit
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCA8979236.638303352564131No Hit
TACGTAGGTGGCAAGCGTTATCCGGAATTATTGGGCGTAAAGCGCGCGTA8900406.580024696901828No Hit
TACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA4179473.089862907280603No Hit
TACGTAGGTGGCGAGCGTTATCCGGATTTACTGGGCGTAAAGGGAGCGTA3901652.8844718617890224No Hit
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA3042432.2492544760454614No Hit
TACGTAGGGGGCTAGCGTTATCCGGAATTACTGGGCGTAAAGGGTGCGTA2527511.868576493378538No Hit
TACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGGGCGCA2527441.8685247427011769No Hit
TACGGAGGATGCGAGCGTTATCCGGAATCATTGGGTTTAAAGGGTCCGTA2309211.707188309551556No Hit
TACGGAGGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA1683401.2445298609910271No Hit
TACATAGGTCGCAAGCGTTATCCGGAATTATTGGGCGTAAAGAGTTCGTA1484861.097750154087642No Hit
TACGGAGGGTGCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTCCGTA1439381.0641269997108616No Hit
TACGTAGGTGGCGAGCGTTGTCCGGATTTACTGGGCGTAAAGGGAGCGTA1154090.8532134176494798No Hit
TACGTAGGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGCGTGTA1142590.8447115206544715No Hit
TACGTATGTCGCAAGCGTTATCCGGATTTATTGGGCGTAAAGCGCGTCTA1142010.8442827293277668No Hit
TACGTAGGTGGCGAGCGTTATCCGGATTTATTGGGCGTAAAGGGAGCGTA1065810.7879484205434516No Hit
TACGTAGGGGGCTAGCGTTATCCGGATTTACTGGGCGTAAAGGGTGCGTA1056110.7807772552520099No Hit
TACGTAGGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGTGCGTA1006730.7442708488508355No Hit
TACGTAGGGTGCGAGCGTTGTCCGGAATTACTGGGCGTAAAGAGCTCGTA994950.7355619491463836No Hit
TACGTAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA980300.7247312716701341No Hit
TACAGAGGGTGCGAGCGTTAATCGGATTTACTGGGCGTAAAGCGTGCGTA924660.6835968761221117No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCGCGCA898400.6641829791578582No Hit
TACAGAGGATGCAAGCGTTATCCGGAATGATTGGGCGTAAAGCGTCTGTA895770.6622386322798695No Hit
TACGAAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGTA858250.6345002692144166No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTACTGGGCGTAAAGGGAGCGTA840340.6212594887639299No Hit
TACGTAGGGTGCGAGCGTTGTCCGGAATTACTGGGCGTAAAGGGCTCGTA808900.5980160416749684No Hit
TACGTATGTCACAAGCGTTATCCGGATTTATTGGGCGTAAAGCGCGTCTA723010.5345179605531201No Hit
TACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGAGCGCA686780.5077332885418899No Hit
TACAGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGTA685830.5070309579205632No Hit
TACGTAGGGGGCAAGCGTTATCCGGAATTACTGGGCGTAAAGGGTGCGTA651950.48198363007787814No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA617310.4563744377381317No Hit
TACGGAGGATACGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA607930.4494398469717684No Hit
TACGGAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGGGCACGCA516600.3819199989235859No Hit
TACATAGGGGGCAAGCGTTATCCGGAATGACTGGGTGTAAAGGGTGCGTA507870.37546595016128836No Hit
TACGTAGGTGGCAAGCGTTATCCGGATTTATTGGGCGTAAAGCGAGCGCA466420.3448221562097153No Hit
TACGTAGGTGGCGAGCGTTATCCGGAATTATTGGGCGTAAAGGGAGCGTA447500.33083468741444966No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA423050.3127589151076714No Hit
TACAGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGAGCGTA392240.2899812241149581No Hit
TACGTAGGGGGCAAGCGTTATCCGGAATTACTGGGTGTAAAGGGTGCGTA390430.28864309945748284No Hit
TACGTATGTCACGAGCGTTATCCGGATTTATTGGGCGTAAAGCGCGTCTA382840.2830318474407774No Hit
TACGTAGGGCGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTCGTA376550.27838167943220343No Hit
TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGTGAGTA374850.2771248772677239No Hit
GACAGAGGATGCAAGCGTTATCCGGAATGATTGGGCGTAAAGCGTCTGTA323870.23943559824115712No Hit
TACGGAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCA318710.2356208340242665No Hit
TACGAAGGGTGCAAGCGTTACTCGGAATTACTGGGCGTAAAGCGTGCGTA317280.23456364161532198No Hit
TACGTAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGAGTGCGCA314260.23233096953489374No Hit
TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA290280.21460266606182446No Hit
TACGTAGGGCGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTTGTA269010.19887785309801362No Hit
TACGTAGGTGACAAGCGTTGTCCGGATTTATTGGGCGTAAAGGGAGCGCA257140.1901024168083834No Hit
TACGTATGTCGCGAGCGTTATCCGGAATTATTGGGCATAAAGGGCATCTA235400.17403013501086356No Hit
TACGTAGGGTGCGAGCGTTGTCCGGATTTATTGGGCGTAAAGGGCTCGTA223710.16538777189159No Hit
TACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGCA213180.15760299142572598No Hit
TACGTAGGTGGCAAGCGTTATCCGGAATTATTGGGCGTAAAGGGTGCGCA201080.1486575171961956No Hit
CACGTAGGTGGCAAGCGTTATCCGGAATTATTGGGCGTAAAGCGCGCGTA200100.14793300771314274No Hit
TACGGAGGGTGCGAGCGTTATTCGGAATTACTGGGCGTAAAGCGCACGCA181990.13454436818448198No Hit
TACGGAGGATGCGAGCGTTATCCGGAATCATTGGGTTTAAAGGGTCTGTA181840.13443347387585147No Hit
TACATAGGATGCAAGCGTTATCCGGATTTACTGGGCGTAAAACGAGCGCA179340.13258523539867575No Hit
TACGTAGGGCGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTCGTA178840.1322155877032406No Hit
TACGGAGGGTGCGAGCGTTAATCGGCATAACTGGGCGTAAAGGGCACGCA175670.12987202131418185No Hit
TACGTAGGTGGCAAGCGTTATCCGGAATTATTGGGCGTAAAGCGCGCGCA162770.12033511077195526No Hit
TACGGAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGCATGCA162640.1202390023711421No Hit
TACGTATGGAGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGGGTACGCA158510.11718571240684784No Hit
TACGAAGGGGGCTAGCGTTGCTCGGAATCACTGGGCGTAAAGGGCGCGTA154270.11405109994955787No Hit
TACGTAGGTGGCAAGCGTTATCCGGCATTATTGGGCGTAAAGCGCGCGTA151500.1120032517168472No Hit
TACGGAGGGTGCGAGCGTTAATCGGAATAACTGGGCGTAAAGCGCACGCA149800.11074644955236773No Hit
TACAGAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGAGTGTA148440.10974100782078415No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGGGAGCGCA147460.10901649833773128No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGTGCA142490.105342200245106No Hit
GACGGGGGGGGCAAGTGTTCTTCGGAATGACTGGGCGTAAAGGGCACGTA136780.1011208235632367No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCGAG5091850.0244.824379
TACGTAT417700.0244.824021
TACGGAG5731700.0244.745681
ACGGAGG5706550.0244.538472
TAGGTCC332000.0244.483445
ACGTATG418100.0244.414022
GGTCCCG332800.0244.411067
AGGATGC688950.0244.039866
ACATAGG262550.0244.020192
AGGTCCC333750.0243.752066
GTAGGGT1335800.0243.422684
TATGTCG162100.0243.110725
TAGGGTG1336950.0243.0855
ATGCGAG528800.0242.938269
ATGTCGC163250.0242.898936
CATAGGT170900.0242.634583
TACGTAG5026200.0242.258121
CGGAGGG5116500.0242.136523
ATGGTGC96900.0241.333856
TCACAAG93950.0241.218739
Produced by FastQC (version 0.11.2)