FastQCFastQC Report
Tue 19 Jul 2016
000000000-APTJP_l01n02_w9.35200000065e9d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-APTJP_l01n02_w9.35200000065e9d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2581287
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGCT2400.017.81254
TAGACTG4850.016.701035
TACACTG8100.016.3888875
CTTACAC4500.016.03
CTAGACT6250.015.8399994
CTATACT10950.015.82191854
AGACTGT4000.015.7500016
TAAGACT6200.015.6048394
ACAGCGT2051.3187673E-915.3658538
CCAGAGC4200.015.03
TAGGCCG2701.8189894E-1214.9999995
TCTAGAC6050.014.8760323
TAGGACT5550.014.5945944
TAATACT16400.014.5426834
CTACACT8200.014.5426834
TATACTG9750.014.5384615
ACTGTGT7750.014.51612958
TTAGACT7300.014.4863014
TTATCCT11400.014.4078934
GCCTAGA3450.014.3478261
Produced by FastQC (version 0.11.2)