FastQCFastQC Report
Tue 19 Jul 2016
000000000-APTJP_l01n02_w8.35200000065e80.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-APTJP_l01n02_w8.35200000065e80.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1927024
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACAG4350.017.0689665
TACCGTG3400.016.5441177
TATACCG4250.016.4117665
TAAGGCT4550.016.3186844
GCCTAGA2800.016.0714281
ACTGTCC2251.8189894E-1116.0000028
CTATACC5650.015.5309744
TAGAGGT4800.015.4687494
AGACTGT3250.015.2307696
ATTCGCG3550.015.21126737
CTGTGCG2101.8535502E-915.0000019
TCTAGCC4050.015.03
GGGTGTC2701.8189894E-1214.9999997
GACTGTG2701.8189894E-1214.9999997
GGTTAGA5450.014.8623861
CTGCCCT4250.014.823534
TATACAG7900.014.8101275
CCTGGAC2900.014.7413793
TTACCCT4750.014.684214
GTATTGG8750.014.6571431
Produced by FastQC (version 0.11.2)