FastQCFastQC Report
Tue 19 Jul 2016
000000000-APTJP_l01n02_w5.35200000065e73.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-APTJP_l01n02_w5.35200000065e73.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2210590
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACACT6900.017.6086944
CTATACT12300.016.4634154
TAAGGCT4700.016.2765964
GTATAGG6900.015.978261
GTATTAG6900.015.6521731
GTAAGGC2900.015.5172413
TCTATAC7200.015.31253
TAGACAG3700.015.20270255
TACACTG5750.014.8695655
GTCTTAG3350.014.7761191
TATACTG7800.014.7115385
AGGACTG3700.014.5945945
ATACTGT8050.014.2546596
CCTATAC8100.014.1666663
TTATCCT9150.014.0163944
CTGTCCG2752.910383E-1113.9090919
GCTATCC4700.013.8829788
CCAGAAC5300.013.5849063
GTATTAT10350.013.4782611
AATACTG8100.013.3333335
Produced by FastQC (version 0.11.2)