FastQCFastQC Report
Tue 19 Jul 2016
000000000-APTJP_l01n02_w4.35200000065e66.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-APTJP_l01n02_w4.35200000065e66.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1132461
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACAG2300.017.6086965
CTATACT4000.016.8754
TATACTG4350.016.5517255
TTATACT6150.016.4634154
TACACTG3450.016.3043485
CCCCTGA1257.6259676E-616.1999991
GTGTTAT2800.016.0714281
CTAGAAC2403.6379788E-1215.9374993
ACTATAC3750.015.63
TATACGG1301.1063617E-515.5769245
CTGTGCG1602.9628427E-715.4687519
GTATTAG3400.015.2205881
ATTATAC5050.015.1485143
ACTGTGC3150.015.08
GGGTTAC1654.1996282E-715.01
GGACCGT1502.573419E-615.06
AAGGCTG2253.0377123E-1015.05
TAGACGT1209.751953E-514.9999994
TACGCTG1209.751953E-514.9999995
AGTACTG4250.014.8235295
Produced by FastQC (version 0.11.2)