FastQCFastQC Report
Tue 19 Jul 2016
000000000-APTJP_l01n02_w3.35200000065e5a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-APTJP_l01n02_w3.35200000065e5a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1299332
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACGG1757.2759576E-1219.2857135
TAACGCC851.2045946E-418.5294114
CTAATAC2950.017.5423743
ATTACGC901.865913E-417.5000023
TAATACT5400.017.54
TAGGACT2450.017.448984
GTAGGAC1459.6702934E-817.0689663
GTCTTAT2650.016.9811331
ATAGTAC4150.016.8072283
GTCTTAG2650.016.1320761
TAGACTG2550.015.8823535
GTATAGG4550.015.8241771
TTACACT3500.015.4285714
TTATACT7200.015.3125014
CTATACT5650.015.1327444
ATAACGC1502.5741265E-615.0000013
TACACTG3650.014.7945195
CCTATAC4900.014.6938773
TCTGTAC3400.014.5588243
GTAAGGG1402.2304086E-514.4642853
Produced by FastQC (version 0.11.2)