FastQCFastQC Report
Tue 19 Jul 2016
000000000-APTJP_l01n02_w2.35200000065e4d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-APTJP_l01n02_w2.35200000065e4d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1902751
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGACTA952.8205733E-416.5789475
TAGACTG2601.0913936E-1114.7115375
CTATACT8350.014.5508984
GACGTTA2203.5743142E-914.31818220
CGTTAGG2203.5743142E-914.31818222
GTCTTAC3150.014.2857141
GTATTAC4400.013.8068181
TATACTA11950.013.7447695
GGACGTT2306.67751E-913.69565319
CACTTCG4500.013.528
GTATTGG7900.013.3860761
ATTACGC1851.5291498E-613.3783783
AAGACAG3700.013.3783785
CTAAGCT5050.013.3663374
ATTCGCG3400.013.23529537
TAAGGCT3600.013.1254
GTATTAT9100.013.1043951
TATACTG6550.013.0534355
TAGACAG2959.640644E-1112.9661025
GTATATA15500.012.9193551
Produced by FastQC (version 0.11.2)