FastQCFastQC Report
Tue 19 Jul 2016
000000000-APTJP_l01n02_w10.35200000065eaa.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-APTJP_l01n02_w10.35200000065eaa.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3087522
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCGT3350.014.1044786
GGACGTT4750.013.73684219
CGTTAGG5300.013.160377522
GACGTTA5000.013.05000120
ACTGTGC7900.012.5316468
GTATTAT13850.012.3465711
GTGTAAG5550.012.1621621
TATACAG9450.012.1428575
ACTGCGC2808.036295E-912.0535728
GTATAGG11050.012.0135761
GTATTAG7500.011.71
GTATTAC7750.011.6129041
CCTATAC10200.011.4705893
ACGTTAG5800.011.25000121
ATTCGCG3652.8376235E-1011.09589137
TTAGACG6300.011.07142838
GTATAGA9200.011.0054341
GCCTTAT5850.010.769231
GTATAAT13450.010.706321
TATACTG10300.010.7038835
Produced by FastQC (version 0.11.2)