FastQCFastQC Report
Tue 19 Jul 2016
000000000-APTJP_l01n02_w1.35200000065e30.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-APTJP_l01n02_w1.35200000065e30.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1071253
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACCGT1608.1672624E-1018.281256
TTAGACT2105.456968E-1217.1428574
ACTGTCC1501.4224497E-716.58
TAGACAG1956.475602E-1016.1538475
TAATACA4200.015.5357144
GTATTGG5450.015.2752291
ATAATAC4450.015.1685393
ACTGTGC2405.0931703E-1115.0000018
TTAACAC2405.0931703E-1115.0000013
TTACGGT1056.036386E-415.04
GTGTAGA3150.014.9999991
TATACTG4100.014.8170735
GCTTTAT2751.8189894E-1214.7272731
GTGTAAC1859.609539E-814.5945961
TAGACTG1402.2296856E-514.4642855
ATTAGAC2651.6370905E-1114.4339633
ACAGTCC1251.380313E-414.4000018
TAGCCTG2509.640644E-1114.4000015
TTATACT5100.014.1176474
ATTCGCG2408.039933E-1014.06250137
Produced by FastQC (version 0.11.2)