FastQCFastQC Report
Tue 19 Jul 2016
000000000-APTJP_l01n01_w9.35100000065e90.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-APTJP_l01n01_w9.35100000065e90.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2581287
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGGGT5750.018.7826084
TATACTG10250.017.7804895
CTATACT12350.017.6720664
AGTACTG7550.016.6887425
TAGGACT5800.015.5172424
TTATACT17300.015.4768794
TAATACT15800.015.2373414
CAGGACC4250.014.8235294
TAAGACT6250.014.7599994
ATACTGT10450.014.42583756
TTAGACT7350.014.3877554
GCGTAGT2203.5761332E-914.3181821
CAGGACT3800.014.2105274
TTAGGAC5550.014.1891893
GTCTTAG4650.014.0322581
TTACACT8050.013.9751564
CCAGGAC4850.013.9175263
TAACCCT7000.013.8214284
TTACCCT5700.013.8157884
CTAGACT5250.013.7142864
Produced by FastQC (version 0.11.2)