FastQCFastQC Report
Tue 19 Jul 2016
000000000-APTJP_l01n01_w5.35100000065e76.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-APTJP_l01n01_w5.35100000065e76.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2210590
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTGCG2800.016.8750029
TATACTG7650.016.7647065
ACTGTGC4450.015.6741588
TATACCG4200.015.05
ATAAGAC6300.014.6428583
CTATACT11300.014.53539854
TAGGACT4200.014.4642864
ACCTGTG3600.014.3757
CCAGGAC4600.014.1847833
TAAGACT5150.013.9805824
TTATACT13050.013.9655184
TTAGTAC6450.013.9534873
TTACGAC1951.8314313E-713.8461533
TAGACTG4600.013.6956535
TTACACT7650.013.5294114
TACTGTG5350.013.4579447
CACTGCG1851.52947E-613.3783787
GTATTAC6650.013.1954891
TACACTG6700.013.0970155
TAAAGGT5000.013.0500014