FastQCFastQC Report
Tue 19 Jul 2016
000000000-APTJP_l01n01_w4.35100000065e69.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-APTJP_l01n01_w4.35100000065e69.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1132461
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGCG654.2381775E-420.76923232
ATACCGC706.983384E-419.2857136
AATACTG3150.017.8571435
ACTGTGC3550.017.7464798
TTAACCT2950.017.5423744
CTGTGCG1953.45608E-1117.3076929
GACTGTG1701.8299033E-917.2058817
GACGTTA1053.1123578E-517.14285720
ATTCGCG1752.6902853E-916.71428737
GTATAGT4450.016.6853921
TAGACTG2057.4578566E-1116.4634155
ACCGCGT1104.6374167E-516.3636388
GTCTAAG1652.357956E-816.3636361
GTATTAT6250.016.21
TAAGACT2650.016.1320764
TAGGGCG1552.0659536E-715.9677425
TAGACAG1855.6188583E-915.8108115
CTAGACT2453.6379788E-1215.6122454
CTTACAC2453.6379788E-1215.6122453
ACTGTCC1602.9628427E-715.4687518