FastQCFastQC Report
Tue 19 Jul 2016
000000000-APTJP_l01n01_w3.35100000065e5d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-APTJP_l01n01_w3.35100000065e5d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1299332
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCCCG901.865913E-417.5000025
ACAGCGC1551.09030225E-817.4193538
TAAGACC1701.8299033E-917.2058814
GTATAGG5500.016.7727281
TAGGGCT2351.8189894E-1216.2765964
ATAATAC5650.015.9292043
ACGTTAG2700.015.83333321
GACGTTA2700.015.83333320
CTATACT5900.015.6355934
TGGACTG2750.015.5454555
GGACGTT2750.015.54545519
TAGAGGT3350.015.4477624
TATACTG4850.015.30927855
TAATACC3900.015.0000014
ATTACGC1351.581867E-514.9999993
CGTTAGG2900.014.7413822
GTATTAA5350.014.7196251
TAGGAGT3250.014.5384624
ATTATAC5150.014.4174763
CTAAGAC2203.5688572E-914.3181823
Produced by FastQC (version 0.11.2)