FastQCFastQC Report
Tue 19 Jul 2016
000000000-APTJP_l01n01_w10.35100000065ead.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-APTJP_l01n01_w10.35100000065ead.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3087522
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGTTA4850.017.62886620
ACGTTAG5150.016.60194221
GTATAGG10750.014.4418611
CGTTAGG5950.013.99159822
GGACGTT6450.013.604650519
TTAGACG6350.013.11023638
AGGACGT7300.012.94520618
TAGGACG6850.012.8102217
TAGGACT6050.012.6446294
TATAGGG9850.012.5634532
CCTATAC11200.012.2544653
TATACCG4650.012.0967745
TGGACTG7450.012.0805385
GTATAAC7550.011.920531
TATACTG10950.011.9178085
CTATACT14600.011.558224
TACACCG5850.011.5384615
GTATAAT14400.011.406251
ATAATAC12400.011.0685483
GTATTAG8450.010.9171591
Produced by FastQC (version 0.11.2)