FastQCFastQC Report
Tue 19 Jul 2016
000000000-APTJP_l01n01_w1.35100000065e33.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-APTJP_l01n01_w1.35100000065e33.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1071253
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAATAC3700.020.0675683
TAATACT5350.017.6635514
GTATTAT3750.017.41
ACTGTGT2850.017.3684228
GTAGACG1305.781676E-717.3076924
TATACCG2159.094947E-1216.7441865
GTATAAG1752.6884663E-916.7142851
CTGTGTA2750.016.3636369
AGTACCG1257.625191E-616.25
CCTGTAC1956.475602E-1016.1538473
TATCCTG2800.016.0714285
TAGCGCT1301.1062508E-515.5769224
CGTAGAC1451.8084447E-615.5172413
TATACAG3350.015.4477625
ATTATAC4850.015.30927853
CTAATAC3100.015.2419353
GGCGTTC1502.573106E-615.08
GTAGCGC1553.6165966E-614.5161293
GAGTCCT1108.587673E-414.3181829
GGGCGTT1108.587673E-414.3181827
Produced by FastQC (version 0.11.2)