FastQCFastQC Report
Wed 15 Jun 2016
000000000-APNWB_l01n03.33300000058c38.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-APNWB_l01n03.33300000058c38.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16822118
Sequences flagged as poor quality0
Sequence length251
%GC49

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTATAGACTTAGTA280719116.687500349242587No Hit
CCTGTTTGCTACCCACGCTTTCGCGCTTTAGCGTCAGTATCTGTCCAGTA5175933.076859881734274No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGATATAGCTTTGTG3163541.88058364588811No Hit
CCTGTTTGATACCCACGCTTTCGTGCTTCAGTGTCAGTTATGGTTTAGTA2810881.6709429811394738No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTCGTCCAGGG2183911.2982372374275344No Hit
CCTGTTTGATTCCCACGCTTTCGTGCTTCAGCGTCAGTGACAGTTTAGTA2062011.2257731160844312No Hit
CCTGTTTGATACCCACACTTTCGTGCCTCAGTGTCAGTTATAGTTTAGTG2026221.2044975549452215No Hit
CCTGTTTGATACCCACACTTTCGTGCATCAGCGTCAGTTATAGTATAGTC1980471.1773012173615713No Hit
CCTGTTTGATCCCCACGCTTTCGTGCTTGAGCGTCAGTTATAGTTTAGTA1630750.9694082516838842No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTTGTCCAGGG1589000.9445897359654712No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG1580000.9392396367686875No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTAAAGCCCAGTA1511650.8986086056464471No Hit
CCTGTTTGATACCCACACTTTCGTGCCTCAGCGTCAGTTATGGTCCAGCC1511030.8982400432573354No Hit
CCTGTTTGATACCCACGCTTTCGCACCTCAGCGTCAGTATTGTGCCAGAT1363260.8103973590008107No Hit
CCTGTTTGATACCCACGCTTTCGCACCTCAGCGTCAGTATAGTGCCAGAT1337650.7951733545086296No Hit
CCTGTTTGATACCCACACTTTCGTGCCTCAGCGTCAGTTATAGGATAGTC1336270.7943530059651227No Hit
CCTGTTTGCTACCCACGCTTTCGCATCTGAGCGTCAGTCATCGTCCAGAA1269050.754393709519812No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAACGTCAGTTACAGTCCAGAA1256230.7467727904417268No Hit
CCTGTTTGTGTTCTGTGCCTTCGTCCCTCAACGTCAGTTATTACATAAAG1248530.7421954833511453No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGTGTCAGTATCAGTCCAGTT1213550.721401431139646No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTATAGACTTAGTT1113730.6620628864926521No Hit
CCTGTTTGATACCCACACTTTCGTGCTTCAGTGTCAGTTGTGGCCTGGTA1077640.6406089887135497No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTATTGTGCCAGAT1024450.6089899024605582No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGGCCAGAG988940.5878807888519151No Hit
CCTGTTTGCTCCCCTAGCTTTCGTGCCTCAGCGTCAGTTGTCACCCAGCA977160.5808781034587915No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGTGTCAGTATCAGTCCAGGT942150.5600662175833031No Hit
CCTGTTCGATCCCCACGCTTTCGTGCCTCAGCGTCAGTAACGGTTTAGTA885660.5264854282914909No Hit
CCTGTTCGCTCCCCACGCTTTCGTGCCTCAGCGTCAGATCCAGCTTAGAA875100.5202079785672649No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTAAAGCCCAGCA873130.5190369012986356No Hit
CCTGTTTGATACCCACACTTTCGTGCATCAGCGTCAGTTATAGTCTGGTC796840.4736858937738993No Hit
CCTGTTTGATCCCCACGCTTTCGCACCTCAGCGTCAGTATTGTGCCAGAT792120.4708800639729195No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGTGTCAGTCTTGGTCCAGAT783680.46586285983726894No Hit
CCTGTTCGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAC749100.44530658981229354No Hit
CCTGTTTGATACCCACACTTTCGTGCCTCAGCGTCAGTTATAGTCTAGAA702590.4176584660742482No Hit
CCTGTTTGATACCCACGCTTTCGTGCCTCAGCGTCAGTTATACTATAGTA698860.4154411471849145No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACAGTCCAGTA676490.40214317840357555No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTAATGTCCAGCA666520.39621645740447187No Hit
CCTGTTTGATACCCACACTTTCGTGCATCAGCGTCAGTTATAGTCCAGTT664100.39477787517600343No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAATGTCCAGCA608530.3617439849131958No Hit
CCTGTTTGATACCCACGCTTTCGCACCTCAGCGTCAGTTTAGTGCCAGAT578260.34374981794801346No Hit
CCTGTTTGATCCCCACGCTTTCGCATCTCAGCGTCAGTACTGCACCAGGT564060.3353085503264215No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCCAATATAGATTCGTC546130.32464996381549577No Hit
CCTGTTTGATACCCACACTTTCGTGCATCAGCGTCAGTTATAGGATAGTC525900.3126241297320587No Hit
CCTATTTGCTCCCCACGCTTTCGGGACTCAGCGTCAGTTTCTGCCCAGAT525470.31236851388154574No Hit
CCTGTTTGATACCCACACTTTCGTGCATCAGCGTCAGTTACACTCCAGTG460260.2736040729235165No Hit
CCTGTTTGATCCCCACGCTTTCGTGCTTGAGCGTCAGTTATAGCTTAGTA455770.27093496787978777No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGAATAAGCTTTGTG449910.26745145884721533No Hit
CCTGTTTGATACCCACACTTTCGTGCCTCAGCGTCAGTTATAGTATAGTA441290.2623272527276292No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTATAGACTTAGTA428920.2549738386093832No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAATGCCCAGCA424000.2520491177151415No Hit
CCTATTTGCTCCCCACGCTTTCGGGACTGAGCGTCAGTTACATCCCAGAT409940.24369107385883276No Hit
CCTGTTTGATACCCACGCTTTCGTGCCTCAGCGTCAGTCGTACTATAGTA401760.23882842814442273No Hit
CCTGTTTGCTCCCCATACTTTCGAGCCTCAGCGTCAGTTGATGCCCAGTA393540.2339420042113603No Hit
CCTGTTTGCTACCCACGCTTTCGTACCTCAGCGTCAGTTTTGTGCCAGGT392600.23338321607302956No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTATCGTGCCAGGT388660.23104106153577095No Hit
CCTGTTTGATCCCCACGCTTTCGTGCTTGAGCGTCAGTTACAGTTTAGTA387390.23028610309355813No Hit
CCTGTTTGATCCCCACGCTTTCGTGCATCAGCGTCAATAATGAATTAGCC386320.22965003574460718No Hit
CCTGTTTGATACCCACACTTTCGTGCATCAGTGTCAGTTGCGACCTGGTA356530.21194120740325326No Hit
CCTGTTTGATCCCCGCGCTTTCGTGCCTCAGCGTCAGTTGCATCCCAGCA352710.20967038752195177No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCATCGTCCAGTA340560.20244775360629383No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTATCAGTCCAGGT339670.20191868824127854No Hit
CCTGTTTGCTACCCACGCTTTCGCACCTCAGCGTCAGTATTGTGCCAGAT339000.20152040307885133No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCCAATATAGATTCGTG319390.189863131384526No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACAGTCCAGTG303240.1802626756036309No Hit
CCTGTTTGATCCCCACGCTTTCGCATCTCAGCGTCAGTACTGCACCAGAT301090.1789845963510659No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACGGTCCAGAA300690.1787468141645422No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCCAACGTAGATTGGTA297340.17675538835240603No Hit
CCTGTTTGATCCCCACGCTTTCGTGCTTCAGCGTCAGTTATAGTTTAGTA283700.16864701579194724No Hit
CCTGTTTGATCCCCACGCTTTCGTGCCTCAGCGTCAATCTAGTATTAGCC278750.16570446123371624No Hit
CCTGTTTGATCCCCACGCTTTCGTGCATCAGCGTCAATAATGAATTAGTC272780.16215556209984974No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATCAGCGTCCGAAATAGATTTGTC271880.16162055218017138No Hit
CCTGTTTGATCCCCACGCTTTCGTGCATGAGCGTCAGTAGTAAGTTTGTA271820.1615848848521928No Hit
CCTGTTTGATACCCACGCTTTCGTGCTTCAGCGTCAGTTGCAGCCTGGTA269650.16029491649030164No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCCAGTA265480.15781603719579188No Hit
CCTGTTTGATACCCACGCTTTCGTGCTTCAGCGTCAGTTGTAGCTTAGTA264380.15716213618285163No Hit
ACTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTATAGACTTAGTA263760.15679357379373987No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCCAATGTAGATTCGTC255460.1518595934233727No Hit
CCTGTTTGCTCCCCATACTTTCGAGCCTCAGCGTCAGTAAATGCCCAGTA253910.15093818745059331No Hit
CCTGTTTGATACCCACACTTTCGTGCATCAGTGTCAGTTACACTTTAGTA253830.15089063101328856No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTATCGTGCCAGAT248840.14792429823640518No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACGTCCCAGCA248700.14784107447112188No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTATGGTCCAGAA243100.14451212385978984No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTAGCGACCCAGGG239040.1420986346665741No Hit
CCTGTTTGATACCCACGCTTTCGTGCTTCAGCGTCAGTGACATTCTAGCA235930.14024987816635218No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTATCGACTTAGTA235850.14020232172904742No Hit
CCTGTTTGCTCCCCTAGCTTTCGTGCCTCAGCGTCAGAAGTGACCCAGCA235570.14003587419848085No Hit
CCCGTTCGCTACCCTGGCTTTCGCGCCTGAGCGTCAGACACAGTCCAGAA232650.13830006423685767No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGATATGGCTTTGTG231930.13787205630111501No Hit
CCTGTTTGATACCCACACTTTCGTGCCTCAGCGTCAGTTATAGCCCAGAA220860.13129143428907109No Hit
CCTGTTTGCTCCCCTAGCTTTCGTGCCTCAGCGTCAGAAGTAACCCAGCA220330.13097637289192715No Hit
CCTGTTTGCTCCCCACGCCTTCGCGCCTCAGCGTCAATATTGGTCCAGAA217570.1293356758049135No Hit
CCTATTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTTACAGACCAGCA217200.12911572728237908No Hit
CCTGTTTGATACCCACACTTTCGTGCCTCAGTGTCAGTAATGGTTTAGTG215930.12836076884016626No Hit
CCTGTTTGATACCCACACTTTCGTGCATCAGCGTCAGTTATAACATAGAA214960.12778414703784624No Hit
CCTGTTCGCTACCCATGCTTTCGTGCTTCAGCGTCAGTTATAGCTTGGTA214560.12754636485132254No Hit
CCTGTTTGATACCCACACTTTCGTGCCTCAGTGTCAGTTACAGTTTAGTA213120.12669034897983714No Hit
CCTGTTTGCTACCCACGCTTTCGCGCTTTAGCGTCAGTATCTGTCCAGTT210790.12530526774333647No Hit
CCTGTTTGATACCCACGCTTTCGTGCTTCAGTGTCAGTTATAGTTTAGTA208900.12418174691201192No Hit
CCTGTTTGATCCCCACGCTTTCGTGCATCAGCGTCAGTAACATCCTAGTG207440.12331384193120035No Hit
CCTGTTTGCTACCCACGCCTTCGCGCCTCAGCGTCAATATTGGTCCAGAA206040.12248160427836732No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTCCAGTA204180.12137591711103204No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTATTGACTTAGTA199660.11868897840331402No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACTGTCCAGTA198250.11785079619581793No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACAGTCCAGCA186590.11091944545865151No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTTAAGTTCTAGTA179100.1064669740159949No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTGTCGGTCCAGAA178600.10616974628284025No Hit
CCTGTTTGATACCCACGCTTTCGTGCTTCAGTGTCAGTTGTAGCTTAGTA177740.10565851458181426No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCCGATGTAGATTTGTA172250.10239495407177622No Hit
CCTGTTTGATACCCACGCTTTCGTGCCTCAGCGTCAGTTGCATCCCAGTA170940.10161621741091104No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTTTGC9448550.0244.867753
TGTTTGA4632500.0244.682683
TTGATAC3309600.0244.555856
TTTGCTC8304800.0244.480775
CTGTTTG14265250.0244.19452
CTGTTCG625350.0243.981372
GTTTGCT9487250.0243.969624
TGTTCGA146850.0243.164783
CTCCCCA8493950.0242.183369
CCTGTTT14203600.0241.871871
CCTGTTC623900.0241.446151
TTTGATA3326700.0241.350815
TTGCTCC8496550.0240.445476
TGATACC3363450.0240.330837
TGTTTGT165250.0240.329793
GCTCCCC8830050.0240.140248
CATACGT367850.0239.43861245
GATACCC3402750.0238.854758
TTGCTAC1501250.0237.215486
TGATCCC1134300.0236.75997
Produced by FastQC (version 0.11.2)