FastQCFastQC Report
Wed 15 Jun 2016
000000000-APNWB_l01n02.33200000058c3b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-APNWB_l01n02.33200000058c3b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16822118
Sequences flagged as poor quality0
Sequence length12
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCCGTTCCAA3584042.130552169471169No Hit
CAGTACGATCTT3549122.1097937845876484No Hit
CGCACCAAGTGA3512762.0881793838326423No Hit
GGAAGCTTGTCT3445872.0484162576912133No Hit
TGATAGTGAGGA3373472.0053776819304203No Hit
ACAATTTCCGAC3350751.9918716537358734No Hit
CTACTACAGGTG3349831.9913247547068686No Hit
CTGTGGATCGAT3335231.982645704898753No Hit
TGTTGACACTAC3310371.9678675420063039No Hit
GTTAAGCCCGAT3300341.9619051536792216No Hit
AAGGGAGGAGAC3226271.917873837289692No Hit
CGCCGTACATAA3207291.9065910725391415No Hit
ATGCCAACCAAC3201161.9029470605306655No Hit
TATAGCCCTGGA3197261.9006286842120592No Hit
GATCCACGGACA3143691.8687837048818705No Hit
TCTAGGAGTTTC3139241.866138378056794No Hit
GCATGCTGGGAT3127671.8592605283115955No Hit
GAACTGTATCTC3110971.8493331220242302No Hit
CCTCCTCTTTAT3092361.8382703057962142No Hit
GTCTCTCTACGC3074821.827843556917149No Hit
ATTGTGCGGTGT3072281.8263336400327235No Hit
CTGATGGGCGTA3056781.8171195803049294No Hit
ATTGCCCAGATG3047041.8113295840630768No Hit
GCACTCGTTAGA3018531.7943816587185988No Hit
GAGTAGCTCGTG3017371.7936920903776803No Hit
TTGTCTGGAAGC3013411.7913380467310953No Hit
GTGAACTTGGGC2996971.7815651988649706No Hit
CCACGTGTGGAA2994901.7803346760497103No Hit
GCGCCGTATAAT2966681.763559142790462No Hit
ACGTGGTTACGT2963641.7617519981728818No Hit
GATGGGATTTCC2950501.7539408533455776No Hit
CGGCGCAAGATT2943341.7496845522068027No Hit
TGGGATGCATGC2938321.7467003857659302No Hit
TCAGGACTGTGT2914061.732278896153267No Hit
TCGGACCTAGGA2905671.7272914147909317No Hit
AGTTGGCCGAGT2845811.6917073105776572No Hit
CGCTTATCGAGA2841101.6889074253313405No Hit
GCTACTTCTTCC2825371.6795566408462952No Hit
AGTACTGCAGGC2818421.6754251753554457No Hit
GTATTGACGGTC2746881.6328978312956786No Hit
ACTGGTTGAGTC2741121.629473767809737No Hit
CATGAGTGCTAC2714891.6138812009284444No Hit
TCAGGTTGCGGA2701941.606183002639739No Hit
AAGATTCGGCGC2682381.5945554537187292No Hit
GTGGACGTCCAA2625551.560772549568372No Hit
TGGAAGATGAGT2569041.5271798711672335No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCTGT1054.8309994E-76.00000054
CGACGGT650.00126198886.00000055
ACGGCAG650.00126198886.00000054
TTCGGCA650.00126198886.00000055
CAGTAGC650.00126198886.00000051
TCCGCGG550.0091608086.03
GATCCCC1750.06.01
GCTGCGG3300.06.05
AAGCGCG600.00339660936.04
TCGATAG550.0091608086.05
TGTGCCG550.0091608086.03
TCTAGCC1653.6379788E-126.01
TGCATCG550.0091608086.04
CAGATTC600.00339660936.01
CGCTACG600.00339660936.05
CAAGGAC600.00339660936.05
CAGGCTT600.00339660936.02
CAGGCCT3800.06.02
AGCGCGA550.0091608086.05
CCGGTTG953.4299446E-66.02
Produced by FastQC (version 0.11.2)